4) If smears are too thick, what might happen? a) The cells might wash off of...
4) If smears are too thick, what might happen?
a) The cells might wash off of the slide during decolorization.
b) The shape of individual cells will be distorted.
c) The smear might stain incorrectly.
d) A and B
Homework Answers
Ans:-) option c. The smear might stain incorrectly because due to thick smear the stain is unable to penetrate.
As the cell gets concentrated in thick smear, light would be unable to due to which it become difficult to see it under microscope. Due to all this, it becomes very difficult to visualise individual cell and to determine cellular morphology.
For proper smear preparation, (i) bacteria should be attached with slide properly so that they are not washed away during decolorisation.
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