Why might it be useful for a protein expression cell line such as the BL21 series,...
Why might it be useful for a protein expression cell line such as the BL21 series, to have genes for several proteases knocked out?
Homework Answers
Protein expression cell line such as BL21 lacks some proteases. So it will not digest recombinant proteins. So T7RNA Polymerase is often employed in BL21 strain from a genomic copy upon induction by IPTG.
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Why might it be useful for a protein expression cell line such
as the BL21 series,...
The BL21(DE3) expression cells carry a λDE3 integration containing the T7-RNA polymerase gene. A) Why is...
The BL21(DE3) expression cells carry a λDE3 integration containing the T7-RNA polymerase gene. A) Why is the T7-RNA polymerase protein so specific with regard to the target genes it transcribes? B) Is this enzyme predicted to transcribe any E. coli genes? Why or why not?
Why might a DNA binding protein cause a protein to be toxic to the cell?
Why might a DNA binding protein cause a protein to be toxic to the cell?
1. why might a protein have multiple domains. 2.why a protein might be comprised of more...
1. why might a protein have multiple domains. 2.why a protein might be comprised of more than one chain. 3.why a protein structure might show gaps when the real protein has none. 4.why a protein might bind nucleic acids. 5.why a protein might have disulfide bonds. 6. are ligands important for function or just artifacts of the structure process? 7. Why would the surface of a membrane-bound protein have a different polarity from a soluble one?
What is the relationship between protein translation and gene expression? Would you expect to always see...
What is the relationship between protein translation and gene expression? Would you expect to always see correlation between these two? Discuss a circumstance within the context of cancer where mRNA and protein expression may not correlate. Why is it important to look at both protein and RNA levels of a series of genes/proteins in a molecular pathway? Discuss this in the context of cancer associated pathways. Give a specific example of a pathway that is independent of gene expression in...
What would happen to the expression of this gene if this cell did not have any...
What would happen to the expression of this gene if this cell
did not have any GAL4 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL80 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL3 protein and why?
Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down...
1. Why are the onion root tip and whitefish blastula useful tissues for studying cell division?...
1. Why are the onion root tip and whitefish blastula useful tissues for studying cell division? 2. What other structures in a plant or animals might be useful for studying mitosis? 3. If a cell has 32 chromosomes when it first enters interphase, how many chromosomes will it have when it starts prophase? 4. What is the purpose of mitosis? In other words, how is it useful for life? 5. How is mitosis different from cytokinesis? 6. How do plant...
4. How does a cell regulate protein activity to impact mechanisms/cell functions? Why is this important?...
4. How does a cell regulate protein activity to impact mechanisms/cell functions? Why is this important? Have five specific examples and do not include any mechanisms that completely degrade the protein of interest.
Cancer and Gene Regulation Why is a cell cycle control system needed for cell division? What...
Cancer and Gene Regulation Why is a cell cycle control system needed for cell division? What happens when cells do NOT respond to the cell cycle control system and divide excessively? Tumor Proto-oncogeno (for protein that stimulates coll division) 6 Y DNA Benign Tumor= Mutation withln a control region of DNA Malignant Tumor Mutated promoter Metastasis Normal growth-stimulating protein in excess Oncogene Tumor-Suppressor Genes Proto-oncogene utled tara gese Samor-auppresr gane Many proto-oncogenes code for growth factors /Deletive nonimenig Normel grewt...
IN CONTEXT OF GENE EXPRESSION AND PROTEIN SYNTHESIS OUTCOME 5. a), Why are Proto-Oncogenes important for...
IN CONTEXT OF GENE EXPRESSION AND PROTEIN SYNTHESIS
OUTCOME
5. a), Why are Proto-Oncogenes important for the cell cycle control and what is their implication in a progression of tumors and cancers? Provide examples of TWO proto-oncogenes and a type of a cancer each is associated with. (6 pts) b) Now consider the diagram below. Under each of three scenarios, explain a likely cellular consequence relative to gene expression and protein synthesis outcome, assuming each could lead to a development...
Suppose you have a cell line that expresses a protein whose structure you know but not...
Suppose you have a cell line that expresses a protein whose structure you know but not the function. Describe an experiment that would help you determine the protein’s function.
What would happen to the expression of this gene if this cell
did not have any GAL4 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL80 protein and why?
What would happen to the expression of this gene if this cell
did not have any GAL3 protein and why?
Understanding regulation of transcription in eukaryotes. The images below depict how the expression of a gene required to break down...
1. Why are the onion root tip and whitefish blastula useful tissues for studying cell division? 2. What other structures in a plant or animals might be useful for studying mitosis? 3. If a cell has 32 chromosomes when it first enters interphase, how many chromosomes will it have when it starts prophase? 4. What is the purpose of mitosis? In other words, how is it useful for life? 5. How is mitosis different from cytokinesis? 6. How do plant...
Cancer and Gene Regulation Why is a cell cycle control system needed for cell division? What happens when cells do NOT respond to the cell cycle control system and divide excessively? Tumor Proto-oncogeno (for protein that stimulates coll division) 6 Y DNA Benign Tumor= Mutation withln a control region of DNA Malignant Tumor Mutated promoter Metastasis Normal growth-stimulating protein in excess Oncogene Tumor-Suppressor Genes Proto-oncogene utled tara gese Samor-auppresr gane Many proto-oncogenes code for growth factors /Deletive nonimenig Normel grewt...
IN CONTEXT OF GENE EXPRESSION AND PROTEIN SYNTHESIS
OUTCOME
5. a), Why are Proto-Oncogenes important for the cell cycle control and what is their implication in a progression of tumors and cancers? Provide examples of TWO proto-oncogenes and a type of a cancer each is associated with. (6 pts) b) Now consider the diagram below. Under each of three scenarios, explain a likely cellular consequence relative to gene expression and protein synthesis outcome, assuming each could lead to a development...
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