Homework Answers
Answer:
coding region:. 5' -ATGGTGCAC- 3'
complimentary sequence: 3'-TACCACGTG-5', on reversing the complementary sequence we get the complimentary strand
complimentary strand: 5'-GTGCACCAT-3'
Because of the nature of complementary base pairing, if you know the sequence of one strand of DNA, you can predict the sequence of the strand that will pair with, and it is known as the complementary strand. A pairs with T and G pairs with C.
Remember to give the correct 5' to 3' orientation
when writing complementary DNA sequences, you need to write the sequence in the 5' to 3' direction. This usually involves reversing the sequence after writing it complementary to the one you are given.
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Sample Question: The start of the coding region for the human B-globulin gene reads 5'-ATGGTGCAC-3'. What...
7. Open reading frames A protein coding gene includes the following sequence on one of its...
7. Open reading frames A protein coding gene includes the following sequence on one of its two DNA strands. Note that this segment (within an exon) does NOT include either the start codon or the stop codon for this gene. However, because five of the six potential reading frames (3 on each DNA strand) in this region include stops, only the true reading frame remains "open" through this segment. Therefore, it is possible to unambiguously decode (translate) this segment of...
Gene Expression Exercise Located below is a gene sequence from the coding strand of DNA 5’ATGCCCGGGTGTCGTAGTTGA3’...
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QUESTION 36 Consider the region of DNA below a segment of a bacterial gene. Several features...
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Below is the partial coding strand of DNA for a gene containing an ORF, shown 5'...
Below is the partial coding strand of DNA for a gene containing
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One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’
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7. Open reading frames A protein coding gene includes the following sequence on one of its two DNA strands. Note that this segment (within an exon) does NOT include either the start codon or the stop codon for this gene. However, because five of the six potential reading frames (3 on each DNA strand) in this region include stops, only the true reading frame remains "open" through this segment. Therefore, it is possible to unambiguously decode (translate) this segment of...
QUESTION 36 Consider the region of DNA below a segment of a bacterial gene. Several features of a "gene have been left out of this segment (eg promoter, terminator). Assume that the direction of movement of the RNA polymerase is from right to left (draw this on a piece of paper so that you can see it S ATAQOCATTCCATACCCAAS AGOTATGGGTT-T True or False The TOP strand serves as the template strand that you can see it) QUESTION / Consider the...
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Below is the partial coding strand of DNA for a gene containing
an ORF, shown 5' to 3'. Identify the regions, shown 1 to 9,
associated with the following genetic elements. Some elements may
have more than one associated region.
(a) The promoter region
(b) The ribosome binding site
(c) The ribosome binding site consensus
sequence
(d) The start codon
(f) The expected region for the +1
transcription (where the transcript
begins to be made)
(g) Is this a prokaryotic...
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