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Utilization of Nutrients Chart Test Test for? Medium +/- Reagents 1. Citrate test +Test/Why? Lab 18-Utilization...
B. Citrate Test. • This is a test for citrate utilization. Identifies bacteria capable of using...
B. Citrate Test. • This is a test for citrate utilization. Identifies bacteria capable of using citrate as a sole carbon source. • These bacteria produce the enzyme Citrate Permease that works in conjunction with Citrate Lyase to produce the end products • Utilization of citrate involves the breakdown of citrate to oxaloacetate and acetate. Oxaloacetate is in turn broken down to Pyruvate and CO, Utilization of ammonium salts in the medium produces ammonium hydroxide that renders the medium alkaline...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
B. Reaction mechanisms (media, reagents, enzymes, products) 1. Media. Match the name of medium with the...
B. Reaction mechanisms (media, reagents, enzymes, products) 1. Media. Match the name of medium with the physiological test. For some tests, multiple media can be used. a. Kligler's iron agar b. MR-VP broth c. Bromocresol Purple (BCP) lactose d. SIM medium e. Simmon's citrate agar f. Skim milk agar 8. Spirit Blue agar h. Tryptone broth _2,3 butanediol fermentation carbohydrate fermentation _casein hydrolysis citrate utilization hydrogen sulfide production mixed acid fermentation triglyceride hydrolysis _tryptophan degradation
1. Medium and culturing of microorganisms - nutrients - growth factors  
1. Medium and culturing of microorganisms - nutrients - growth factors - rich versus minimal medium - agar: What is it? Why do we use it? - sterilization (autoclave) - aseptic technique: why do we do it?
Test or Medium Purpose API System Enterotube Citrate Glucose H2S Indole Lysine decarboxylase Omithine decarboxylase Urease...
Test or Medium Purpose API System Enterotube Citrate Glucose H2S Indole Lysine decarboxylase Omithine decarboxylase Urease T T T Arial 3 (12pt) T-. E < 1 * Words 0 List 4 carbohydrates routinely used to determine the fermentative ability of bacterial species. a. b. C. d. TT T Arial 3 (12pt)
PRE-LAB ASSIGNMENT 1) Use the General Solubility Guidelines provided to complete the chart. Set # Reagents Used Obs...
PRE-LAB ASSIGNMENT 1) Use the General Solubility Guidelines provided to complete the chart. Set # Reagents Used Observation (ppt. or no ppt.) 2 NaCl and Fe(NO3)2 Pb(NO3)2 and MgCl2 AgNO, and KCI NHOH and LICI 2) A student mixes aqueous solutions of NaCrOs and Pb(NO3)2. Using your Solubility Guidelines determine if a precipitate will form. If a precipitate forms, write its correct formula and name. Write the names and symbols of any spectator ions that may form.
Micro Lab: Enterbacteriacease Lab 7; Enterobacteriacease Question TSI/SIM Yersinia pestis ! Name the sugars in a...
Micro Lab: Enterbacteriacease
Lab
7; Enterobacteriacease Question TSI/SIM
Yersinia pestis ! Name the sugars in a TSI slant. 20. 21. TSI slants are incubated with the caps loose/tight. 22. Most bacteria will grow best at 35 degrees Centigrade. There are two exceptions. Yersinia will grow at while Campylobacter is incubated at __degrees centigrade. 23. Name an organism that gives each TSI result. A/A gas-- K/A-- K/A H2S+-- K/N- what three tests can be determined from SIM media? List an organism...
catalase test EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart....
catalase test
EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart. Bacteria Foaming or No Foaming Catalase Present Absent M. luteus S. aureus S. lactis 1. Review Questions: 1. Explain why the enzyme catalase is essential for the survival of many aerobic and facultative anaerobic organisms? 2. Explain how catalase negative bacteria are capable of growing in the presence of oxygen Experiment 30 Catalase Test 273 3. Can bacterial cultures grown on blood agar be...
18. You have carried out the ferric chloride test as part of one of your lab...
18. You have carried out the ferric chloride test as part of one of your lab experiment. chromate + 3 cht igre (a) What functional group(s) does it test for the presence of? Solsonarollo absmedarmo (b) What is the reaction that occurs if that functional group is present? bio immoned OSHO lo summo imalo seed broquinon 21 barem nolo 90 World of bobben envoltorio) Sushiba Holland worto (c) What are strong choices for positive and negative controls? Lobbs Sbrilog og...
Unit 2, Lab 1: Flame Test Name: &
Unit 2, Lab 1: Flame Test Name: Date: Lab Results Record the results of your flame tests. Compounds Flame Color lithium strontium calcium magnesium zinc copper barium manganese lead bismuth arsenic potassium Conclusions Why do different elements produce distinct colors in the flame test? Make sure to include a discussion of electrons in your answer (3 sentences required). Explain a reason why a flame test would not match the expected colors listed in the background
B. Citrate Test. • This is a test for citrate utilization. Identifies bacteria capable of using citrate as a sole carbon source. • These bacteria produce the enzyme Citrate Permease that works in conjunction with Citrate Lyase to produce the end products • Utilization of citrate involves the breakdown of citrate to oxaloacetate and acetate. Oxaloacetate is in turn broken down to Pyruvate and CO, Utilization of ammonium salts in the medium produces ammonium hydroxide that renders the medium alkaline...
Citrate Utilization Test OBSERVATIONS AND INTERPRETATIONS 1 Using Table 5-10, page 340, as a guide, enter your results in the table below. Organism Color Result (+ or -) Interpretation Control QUESTIONS 1 Consider the uninoculated tube. a. Is it a positive or a negative control? b. What information is provided by the uninoculated control? Rus. ay Manry bacteria that are able to metabolize citrate (as seen in the citric acid cycle) produce negative e this test. Why? Be specific. ative...
B. Reaction mechanisms (media, reagents, enzymes, products) 1. Media. Match the name of medium with the physiological test. For some tests, multiple media can be used. a. Kligler's iron agar b. MR-VP broth c. Bromocresol Purple (BCP) lactose d. SIM medium e. Simmon's citrate agar f. Skim milk agar 8. Spirit Blue agar h. Tryptone broth _2,3 butanediol fermentation carbohydrate fermentation _casein hydrolysis citrate utilization hydrogen sulfide production mixed acid fermentation triglyceride hydrolysis _tryptophan degradation
Test or Medium Purpose API System Enterotube Citrate Glucose H2S Indole Lysine decarboxylase Omithine decarboxylase Urease T T T Arial 3 (12pt) T-. E < 1 * Words 0 List 4 carbohydrates routinely used to determine the fermentative ability of bacterial species. a. b. C. d. TT T Arial 3 (12pt)
PRE-LAB ASSIGNMENT 1) Use the General Solubility Guidelines provided to complete the chart. Set # Reagents Used Observation (ppt. or no ppt.) 2 NaCl and Fe(NO3)2 Pb(NO3)2 and MgCl2 AgNO, and KCI NHOH and LICI 2) A student mixes aqueous solutions of NaCrOs and Pb(NO3)2. Using your Solubility Guidelines determine if a precipitate will form. If a precipitate forms, write its correct formula and name. Write the names and symbols of any spectator ions that may form.
Micro Lab: Enterbacteriacease
Lab
7; Enterobacteriacease Question TSI/SIM
Yersinia pestis ! Name the sugars in a TSI slant. 20. 21. TSI slants are incubated with the caps loose/tight. 22. Most bacteria will grow best at 35 degrees Centigrade. There are two exceptions. Yersinia will grow at while Campylobacter is incubated at __degrees centigrade. 23. Name an organism that gives each TSI result. A/A gas-- K/A-- K/A H2S+-- K/N- what three tests can be determined from SIM media? List an organism...
catalase test
EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart. Bacteria Foaming or No Foaming Catalase Present Absent M. luteus S. aureus S. lactis 1. Review Questions: 1. Explain why the enzyme catalase is essential for the survival of many aerobic and facultative anaerobic organisms? 2. Explain how catalase negative bacteria are capable of growing in the presence of oxygen Experiment 30 Catalase Test 273 3. Can bacterial cultures grown on blood agar be...
18. You have carried out the ferric chloride test as part of one of your lab experiment. chromate + 3 cht igre (a) What functional group(s) does it test for the presence of? Solsonarollo absmedarmo (b) What is the reaction that occurs if that functional group is present? bio immoned OSHO lo summo imalo seed broquinon 21 barem nolo 90 World of bobben envoltorio) Sushiba Holland worto (c) What are strong choices for positive and negative controls? Lobbs Sbrilog og...
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