Question

1. The following nucleotide sequence is found on a strand of mRNA. Give the altered amino acid sequence of the protein that will be found in each of the following mutations. Please also list an anticipated phenotypical change(s) (nonsense, missense, frameshift, addition/subtraction of amino acid etc.) (1 pts each) Second position Nucleotide #: 1 4 7 10 13 U UUU 16 19 22 UCU UAU UGU UUC UAC UAA UUA UUD RNA: 5-AUG ACC GGC AAU CAA CUA UAU UGA-3' A) Translate the partial mRNA sequence to a Polypeptide: UCO CCU GUU CUC CCC OCA B) Mutant 1: A one nucleotide deletion at nucleotide 7 First position CUA CUO CCG ACU AUC ile ACC AUA AUG met ACG GUU GCU GUC GUA QCA GUG GCG GAU GGU GCC GACAP GGC GAN GGA GAG GGG C) Mutant 2: Single base substitution at nucleotide 11 from A to G Initiation Termination D) Mutant 3: Single base substitution at nucleotide 13 from C to U E) Mutant 4: Single base substitution at nucleotide 9 from Cto A F) Mutant 5: Insertion of ACC after nucleotide 6

Answer 1

A)N-Met the gly ass ger his tyr stop -c New RNA, AUG ACC GCA AUC AAC UAU AVU GA N- met thee ala ile ash tyr ile-c Frameshift - addition c) New RNA, AUG ACC GGC AGU CAA CUA UAU UGA? N-met the gly ser gin his tyr-c Point mutan, musseuse 0 AUG ACC 99C AAU VAA CUA VAU OGA met the gly asa stop Nonsense E) AUG ACC GGA AAU CAA CUA VAUUGA? met thee gly am gen his tyo stop - silent F) AUG ACC ACC 99A AAU CAA CUA VAV UGAR met the the gly ass glon his tyr stop Insertion framesligt

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