Glyceraldehyde-3-phosphate dehydrogenase catalyzes the phosphorylation of glyceraldehyde-3-phosphate, but unlike other glycolytic reactions, it does not require...
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the phosphorylation of glyceraldehyde-3-phosphate, but unlike other glycolytic reactions, it does not require energy investment from ATP. This reaction occurs in two stages: oxidation/reduction and phosphorylation. Why is the oxidation/reduction reaction necessary to make phosphorylation favorable? Choose one:
A. The oxidation/reduction reaction is necessary to oxidize phosphate, allowing addition to the substrate.
B. The oxidation/reduction reaction is necessary to form an intermediate with a large free energy of hydrolysis.
C. The oxidation/reduction reaction is necessary to produce NADH, which is required for ATP synthesis.
D. The oxidation/reduction reaction is for the substrate to bind to the enzyme.
Homework Answers
This oxidation/reduction reaction is necessary because it forms an intermediate with a large free energy of hydrolysis which drives the phosphorylation reaction because the phosphorylation need energy but the dehydrogenase does not use ATP for that.So it needs the energy and by making an intermediate , it gets the energy for further reaction.
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Glyceraldehyde-3-phosphate dehydrogenase catalyzes the
phosphorylation of glyceraldehyde-3-phosphate, but unlike other
glycolytic reactions, it does not require...
Question 3 (4 points) The enzyme glyceraldehyde 3-phosphate dehydrogenase catalyzes the reaction glyceraldehyde 3- phosphate →...
Question 3 (4 points) The enzyme glyceraldehyde 3-phosphate dehydrogenase catalyzes the reaction glyceraldehyde 3- phosphate → 1,3 diphosphoglycerate. The region of the enzyme where glyceraldehyde 3-phosphate binds is called the transition state. O groove. catalyst. active site.
GLYCOLYSIS Glucose-8 phosphate + Glucose 2 Fructose-6-phosphate 3 Fructose-1,6-biphosphate Dihydroxy-acetone-phosphate 5 Glyceraldehyde-3-phosphate VT6 2 (1,3-biphosphoglycerate) 7...
GLYCOLYSIS Glucose-8 phosphate + Glucose 2 Fructose-6-phosphate 3 Fructose-1,6-biphosphate Dihydroxy-acetone-phosphate 5 Glyceraldehyde-3-phosphate VT6 2 (1,3-biphosphoglycerate) 7 3-phosphoglycerate 2-phosphoglycerate 9 phosphoenolpyruvate 10 Lactate Pyruvate Which two reactions of glycolysis requires an investment of ATP energy, and which enzyme catalyzes each reaction? Reaction Number Enzyme Reaction Number Enzyme Which two reactions of glycolysis generate energy in the form of ATP, and which enzyme catalyzes each reaction? Reaction Number Enzyme Reaction Number Enzyme Which glycolytic reaction generates energy in the form of NADH,...
The mechanism of glyceraldehyde 3 phosphate dehydrogenase. In step 1 (Top Left) the sulfur from the cysteine loses its...
The mechanism of glyceraldehyde 3 phosphate dehydrogenase. In
step 1 (Top Left) the sulfur from the cysteine loses its hydrogen
to the carbonyl carbon of the substrate. I think is wrong and the
hydrogen is stolen by the histidine (nitrogen) using a base
catalyst reaction.
lser Glyceraldehyde 3-phosphate NAD CONH2 H -N + HH Hemithioacetal Oxidation NAD NADH CONH CONH2 Но RN + H -N / H Η Η Hн s NADH NAD Thioester intermediate Thioester intermediate Phosphorylation CONH2 O...
In the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, NADH is made and phosphate is added. What drives...
In the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, NADH is made and phosphate is added. What drives this reaction? O The hydrolysis of ATP to phosphorylate carbon 1 of glyceraldehyde-3-phosphate. The reduction of glyceraldehyde-3-phosphate. The reduction of NAD+. O The oxidation of glyceraldehyde-3-phosphate.
14. Which step of reaction requires inorganic phosphate? What is the enzyme? Which enzyme category does...
14. Which step of reaction requires inorganic phosphate? What is the enzyme? Which enzyme category does it belong to? 15. Which step of reaction produces H20 as a byproduct? P-0-CH CH - Glyceraldehyde 3-phosphate Dihydroxyacetone phosphate triose phosphate -O-CH2 -CHCH isomerase 2NAD Ilyceraldehyde oxidation and (b) Payoff phase Oxidative conversion of (2) Glyceraldehyde (21 -0-CH -CH glyceraldehyde 3-phosphate 3-phosphate to pyruvate and the coupled 2P IN 6 formation of ATP and NADH 2NAD I 3 phosphate Jl dehydrogenase phosphorylation 2...
1. 2. 3. 4. Select all that apply. Identify the reaction(s) of glycolysis inhibited by ATP □ glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate phosphoenolpyruvate +pyruvate 3-phosphoglycerate...
1.
2.
3.
4.
Select all that apply. Identify the reaction(s) of glycolysis inhibited by ATP □ glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate phosphoenolpyruvate +pyruvate 3-phosphoglycerate ~2-phosphoglycerate 1) fructose-6-phosphate→ fructose-1,6-bisphosphate . glucose glucose-6-phosphate Select all that apply. Alcohol fermentation: consumes ATP as it produces ethanol. O produces carbon dioxide begins as pyruvate is decarboxylated to acetaldehyde in a reaction catalyzed by alcohol dehydrogenase m regenerates NAD requires the enzyme alcohol dehydrogenase, In the figure below, what is the reaction occurring at the location...
4. What is the commited step of glycolysis and how is this enzyme regulated? 5. Glyceraldehyde-3-phophate...
4. What is the commited step of glycolysis and how is this enzyme regulated? 5. Glyceraldehyde-3-phophate dehydrogenase (GAPDH) is the most important enzyme in glycolysis. a. What two reactions are coupled by GAPDH? b. Draw the structure of the thiohemiacetal intermediate and show the mechanism for generating the acyl thioester intermediate. A+Bred Ared +B For the reaction: AG = Given: ngAE RT In n[Aj{8,al Nernst Equation: AE=A Use the reduction potentials in Table 14-4 to calculate the electromotive force, AE,...
NAD+ -dependent Glyceraldehyde-3-phosphate + Dehydrogenase from Thermoproteus tenax. 1. Name the three enzymes that catalyze irreversible,...
NAD+ -dependent Glyceraldehyde-3-phosphate + Dehydrogenase from Thermoproteus tenax. 1. Name the three enzymes that catalyze irreversible, regulated reactions in glycolysis as studied in class. 2. What is the significance of the GAPDH reaction in E. coli to glycolysis? 3. How does the reaction catalyzed by GAPDH from T. tenax presented here differ from the reaction carried out in E. coli? 4. The activity of the GAPDH enzyme was assayed in the presence of a constant amount of glyceraldehyde-3-phosphate and an...
First question: Which type of enzyme fron table 11-2 catalyzes the following reactions (a) 600 c=0...
First question: Which type of enzyme fron table 11-2 catalyzes the
following reactions
(a) 600 c=0 CH + NADH + H - 500- + NAD HO-- CH, COO H-CH(CH HỘI NH ADP + P 5. On the free energy diagram shown, label the intermediate(s) and transition state(s). Is the reaction thermodynamically favorable? 6. Urcase, the first enzyme to be crystallized, is inhibited in the presence of Hg, Cd, or Co ions What does this information suggest about the catalytic mechanism...
1220 Which is not a result of one pass through the citric acid cycle? A. 2...
1220 Which is not a result of one pass through the citric acid cycle? A. 2 molecules of Co, are produced. B. 3 molecules of NADH are produced from NAD C. 1 substrate-level phosphorylation occurs to produce GTP or ATP. D. 2 QH, are produced by the succinate dehydrogenase complex. E. The oxidation of L-malate regenerates oxaloacetate. 2012 Which enzyme catalyzes a substrate-level phosphorylation reaction? A. Isocitrate dehydrogenase B. Succinyl-CoA synthetase C. Fumarase D. Aconitase E. Citrate synthase 14.30 What...
Question 3 (4 points) The enzyme glyceraldehyde 3-phosphate dehydrogenase catalyzes the reaction glyceraldehyde 3- phosphate → 1,3 diphosphoglycerate. The region of the enzyme where glyceraldehyde 3-phosphate binds is called the transition state. O groove. catalyst. active site.
GLYCOLYSIS Glucose-8 phosphate + Glucose 2 Fructose-6-phosphate 3 Fructose-1,6-biphosphate Dihydroxy-acetone-phosphate 5 Glyceraldehyde-3-phosphate VT6 2 (1,3-biphosphoglycerate) 7 3-phosphoglycerate 2-phosphoglycerate 9 phosphoenolpyruvate 10 Lactate Pyruvate Which two reactions of glycolysis requires an investment of ATP energy, and which enzyme catalyzes each reaction? Reaction Number Enzyme Reaction Number Enzyme Which two reactions of glycolysis generate energy in the form of ATP, and which enzyme catalyzes each reaction? Reaction Number Enzyme Reaction Number Enzyme Which glycolytic reaction generates energy in the form of NADH,...
The mechanism of glyceraldehyde 3 phosphate dehydrogenase. In
step 1 (Top Left) the sulfur from the cysteine loses its hydrogen
to the carbonyl carbon of the substrate. I think is wrong and the
hydrogen is stolen by the histidine (nitrogen) using a base
catalyst reaction.
lser Glyceraldehyde 3-phosphate NAD CONH2 H -N + HH Hemithioacetal Oxidation NAD NADH CONH CONH2 Но RN + H -N / H Η Η Hн s NADH NAD Thioester intermediate Thioester intermediate Phosphorylation CONH2 O...
In the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, NADH is made and phosphate is added. What drives this reaction? O The hydrolysis of ATP to phosphorylate carbon 1 of glyceraldehyde-3-phosphate. The reduction of glyceraldehyde-3-phosphate. The reduction of NAD+. O The oxidation of glyceraldehyde-3-phosphate.
14. Which step of reaction requires inorganic phosphate? What is the enzyme? Which enzyme category does it belong to? 15. Which step of reaction produces H20 as a byproduct? P-0-CH CH - Glyceraldehyde 3-phosphate Dihydroxyacetone phosphate triose phosphate -O-CH2 -CHCH isomerase 2NAD Ilyceraldehyde oxidation and (b) Payoff phase Oxidative conversion of (2) Glyceraldehyde (21 -0-CH -CH glyceraldehyde 3-phosphate 3-phosphate to pyruvate and the coupled 2P IN 6 formation of ATP and NADH 2NAD I 3 phosphate Jl dehydrogenase phosphorylation 2...
1.
2.
3.
4.
Select all that apply. Identify the reaction(s) of glycolysis inhibited by ATP □ glyceraldehyde-3-phosphate → 1,3-bisphosphoglycerate phosphoenolpyruvate +pyruvate 3-phosphoglycerate ~2-phosphoglycerate 1) fructose-6-phosphate→ fructose-1,6-bisphosphate . glucose glucose-6-phosphate Select all that apply. Alcohol fermentation: consumes ATP as it produces ethanol. O produces carbon dioxide begins as pyruvate is decarboxylated to acetaldehyde in a reaction catalyzed by alcohol dehydrogenase m regenerates NAD requires the enzyme alcohol dehydrogenase, In the figure below, what is the reaction occurring at the location...
4. What is the commited step of glycolysis and how is this enzyme regulated? 5. Glyceraldehyde-3-phophate dehydrogenase (GAPDH) is the most important enzyme in glycolysis. a. What two reactions are coupled by GAPDH? b. Draw the structure of the thiohemiacetal intermediate and show the mechanism for generating the acyl thioester intermediate. A+Bred Ared +B For the reaction: AG = Given: ngAE RT In n[Aj{8,al Nernst Equation: AE=A Use the reduction potentials in Table 14-4 to calculate the electromotive force, AE,...
First question: Which type of enzyme fron table 11-2 catalyzes the
following reactions
(a) 600 c=0 CH + NADH + H - 500- + NAD HO-- CH, COO H-CH(CH HỘI NH ADP + P 5. On the free energy diagram shown, label the intermediate(s) and transition state(s). Is the reaction thermodynamically favorable? 6. Urcase, the first enzyme to be crystallized, is inhibited in the presence of Hg, Cd, or Co ions What does this information suggest about the catalytic mechanism...
1220 Which is not a result of one pass through the citric acid cycle? A. 2 molecules of Co, are produced. B. 3 molecules of NADH are produced from NAD C. 1 substrate-level phosphorylation occurs to produce GTP or ATP. D. 2 QH, are produced by the succinate dehydrogenase complex. E. The oxidation of L-malate regenerates oxaloacetate. 2012 Which enzyme catalyzes a substrate-level phosphorylation reaction? A. Isocitrate dehydrogenase B. Succinyl-CoA synthetase C. Fumarase D. Aconitase E. Citrate synthase 14.30 What...
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