Question

Suppose it was possible to change the anticodon on a tRNA from 3-CCG-5'to 3-UGG- 5' after the amino acid was already loaded. Predict the result in a translated polypeptide. What if you changed the sequence prior to the amino acid being loaded onto the tRNA? O In the first case glycine would be found in the polypeptide where threonine was supposed to be. In the second case the changed tRNA would behave like a tRNA for threonine. O In the first case there would be no change since the amino acid was already loaded. In the second case threonine would be found in the polypeptide where glycine was supposed to go. O In the first case threonine would be found in the polypeptide where glycine was supposed to be. In the second case the changed tRNA would behave like a tRNA for glycine. O In the first case tryptophan would be found in the polypeptide where proline was supposed to be. In the second case the changed tRNA would behave like a tRNA for tryptophan.

Answer 1

Since the amino acid is already loaded , there will be no change , it will code for glycine. In the second case when the anticodon sequence is changed before amino acid is being loaded on to tRNA, threonine would be found in the poly peptide where glycine was supposed to be. So the answer is option B.