how to get ride of contaminated CHO cells from bioreactor
Applying direct electric current (approx 0.6 A) CHO contaminants in the bioreactor can be inhibited, cell death rate is proportional to the current. In addition well-designed and validated system, skilled operating staff, cleaning of the equipments should be monitored.
A CHO mammalian cell culture is set up by a chemostat stirred tank bioreactor to produce HBsAg. Glucose is the growth rate limited substrate and cell growth follows the Monod Model. The feeding flow rate is 20L/hr with the glucose concentration 0.25M, and the glucose concentration in the outlet is 5g/L. It has been found out the maximal cell growth rate is 0.2 hr-1 and the saturation constant, or half-velocity constant of substrate is 2.0 g/L. The cell yield coefficient...
1. You are working on tissue engineering cartilage in a bioreactor by seeding mesenchymal stem cells (MSCs) on a scaffold. a) What growth factor would you use to promote the MSC differentiation into chondrocytes? Fibroblast b) What are the advantages and disadvantages of directly injecting growth factors into the cell-seeded scaffold? We were unable to transcribe this image
1. You prepared a dilution 1 in 2 of trypsinized CHO-K1 cells. Then, you took an aliquot of the cell suspension and counted the cells using a hemocytometer. There were 75 cells in the 16 little squares. Please explain how to get each! a. Calculate the concentration of the cells prior to the dilution. Show your calculations. Use scientific notation to express the result. b. Now, you want to seed a 25cm2 flask with 750,000 cells. Calculate how many ml...
A precipitate of Fe(OH)3 is contaminated with Mg(OH)2. Explain how to can get rid of the contamination.
why weaning CHO cells off DMEN medium onto CD CHO medium important
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML...
1. You are working on tissue engineering cartilage in a bioreactor by seeding mesenchymal stem cells (MSCs) on a scaffold. a) What growth factor would you use to promote the MSC differentiation into chondrocytes? Fibroblast b) What are the advantages and disadvantages of directly injecting growth factors into the cell-seeded scaffold? c) Can you propose another approach to provide growth factors to the tissue engineering construct? What are the advantages of your proposal? adatantages; grouth Factros can also scaffolds through...
can someone outline in detail the factours affecting growth in a bioreactor and how to control them e.g pH,temperature,dissolved oxygen
1. You are working on tissue engineering cartilage in a bioreactor by seeding mesenchymal stem cells (MSCs) on a scaffold. a) What growth factor would you use to promote the MSC differentiation into chondrocytes? b) What are the advantages and disadvantages of directly injecting growth factors into the cell-seeded scaffold? c) Can you propose another approach to provide growth factors to the tissue engineering construct? What are the advantages of your proposal? d) Besides growth factors, what other regulators would...
In what sense do firms with nontradable assets get a free-ride from firms whose securities are internationally tradable? explain in detail also give example