What are the two possible primary electron donors when E. coli is grown aerobically on glucose?
What are the two possible primary electron donors when E. coli is grown aerobically on glucose?
Homework Answers
When e coli is grown aerobically on glucose.it is converted into co2 and water using oxygen., it is completely oxidized by glycolysis into pyruvate then . Krebs cycle and electron transport chain operates and ATP is formed
Primary electron donors are NADH.
Glucose may be the electron donor.
Add Answer to:
What are the two possible primary electron donors when E. coli
is grown aerobically on glucose?
When E. coli is growing aerobically and is fed glucose that contains 13C (a stable isotope...
When E. coli is growing
aerobically and is fed glucose that contains 13C (a stable isotope
of the normal 12C) at the C3 and C4 positions, half the pyruvate
generated has the 13C in the carboxylic acid portion of the
molecule with the other half having the 13C in the methyl
group. When the bacterium is switched to growing
anaerobically and is fed the same 13C-labeled glucose, all of the
pyruvate has the 13C as the carboxylic acid portion of the...
E. coli is grown in nutrient broth in a shaker and is harvested by centrifugation in...
E. coli is grown in nutrient broth in a shaker and is harvested by centrifugation in mid- exponential growth phase. After washing, half of the cells are placed back into fresh nutrient broth and the other half are placed into fresh glucose-salts minimal medium. All other conditions are kept the same Sketch growth curves for the two cultures that clearly diagram the probable differences one would observe.
You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity...
You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity of a culture grown in glycerol minimal medium is the same as a culture grown in lactose minimal medium. Is this expected? True or false: Glycerol is another possible carbon source for E. coli and it is not either glucose or lactose.
3. Yeast can grow on glucose either anaerobically or aerobically. This choice is not available when...
3. Yeast can grow on glucose either anaerobically or aerobically. This choice is not available when yeast grow on glycerol: yeast can grow only aerobically on this carbon source. Glycerol is metabolized to form dihydroxyacetone phosphate (DHAP) by the following two reactions: glycerol + ATP →glycerol 3-phosphate + ADP glycerol 3-phosphate + NAD → DHAP + NADH + H+ This DHAP then enters the glycolytic pathway. Based on this information, please explain why yeast cannot grow on glycerol under anaerobic...
In E coli the lac operon will express enzymes for the digestion of (Glucose/Lactose) when it...
In E coli the lac operon will express enzymes for the digestion of (Glucose/Lactose) when it is present, but if both the sugar mentioned before and (Glucose/ Lactose) are present the lac operon is repressed, resulting in the (Glucose/ Lactose) being used first. If there are high levels of cAMP, this means (Glucose/Lactose) levels are low, this results in a (Represser/Promoter) being activated and (Decreasing/Increasing) transcription of the lac operon.
An E. coli culture is grown in a variety of very rich media. With a number...
An E. coli culture is grown in a variety of very rich media. With a number of the media you are able to reduce the generation time to 20 mins. No further additions to the media reduce the doubling time below 20 mins. The supply of nutrients and building blocks are not limited. What factors could be limiting the growth rate of the cell?
A batch culture of E. coli was initiated with a medium containing 10 g/L of glucose,...
A batch culture of E. coli was initiated with a medium
containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of
cells
A batch culture of E. coli was initiated with a medium containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of cells. At the end of culture, 0.1 g/L of glucose remained in the medium. A total of 5.1 g/L of E. coli were recovered. The elemental composition of the cells was...
At what levels of glucose and lactose should E. coli produce galactoside permease and beta-galactosidase? (2...
At what levels of glucose and lactose should E. coli produce galactoside permease and beta-galactosidase? (2 marks)
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli...
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli which is cys+ ton trp- to infect a recipient strain of E. coli which is cys- tons trp+. These bacteria are then plated on complete media with phage T1 (tons sensitive to phage T1). You obtain 50 ton? transductants which you go on to test on minimal media for the other markers. cyst trp+ cyst trp- cys- trpt cys- trp- 10 24 13 a....
5 points. When lambda infects an E. coli host what are the first two proteins that...
5 points. When lambda infects an E. coli host what are the first two proteins that are produced? 5 points. In a lambda lysogen, what proteins are produced?
When E. coli is growing
aerobically and is fed glucose that contains 13C (a stable isotope
of the normal 12C) at the C3 and C4 positions, half the pyruvate
generated has the 13C in the carboxylic acid portion of the
molecule with the other half having the 13C in the methyl
group. When the bacterium is switched to growing
anaerobically and is fed the same 13C-labeled glucose, all of the
pyruvate has the 13C as the carboxylic acid portion of the...
E. coli is grown in nutrient broth in a shaker and is harvested by centrifugation in mid- exponential growth phase. After washing, half of the cells are placed back into fresh nutrient broth and the other half are placed into fresh glucose-salts minimal medium. All other conditions are kept the same Sketch growth curves for the two cultures that clearly diagram the probable differences one would observe.
3. Yeast can grow on glucose either anaerobically or aerobically. This choice is not available when yeast grow on glycerol: yeast can grow only aerobically on this carbon source. Glycerol is metabolized to form dihydroxyacetone phosphate (DHAP) by the following two reactions: glycerol + ATP →glycerol 3-phosphate + ADP glycerol 3-phosphate + NAD → DHAP + NADH + H+ This DHAP then enters the glycolytic pathway. Based on this information, please explain why yeast cannot grow on glycerol under anaerobic...
A batch culture of E. coli was initiated with a medium
containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of
cells
A batch culture of E. coli was initiated with a medium containing 10 g/L of glucose, 2 g/L of ammonia, and 0.1 g/L of cells. At the end of culture, 0.1 g/L of glucose remained in the medium. A total of 5.1 g/L of E. coli were recovered. The elemental composition of the cells was...
3. (4) Suppose that you use a phage P1 grown on a strain of E. coli which is cys+ ton trp- to infect a recipient strain of E. coli which is cys- tons trp+. These bacteria are then plated on complete media with phage T1 (tons sensitive to phage T1). You obtain 50 ton? transductants which you go on to test on minimal media for the other markers. cyst trp+ cyst trp- cys- trpt cys- trp- 10 24 13 a....
5 points. When lambda infects an E. coli host what are the first two proteins that are produced? 5 points. In a lambda lysogen, what proteins are produced?
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