At what levels of glucose and lactose should E. coli produce galactoside permease and beta-galactosidase? (2...
At what levels of glucose and lactose should E. coli produce galactoside permease and beta-galactosidase? (2 marks)
Homework Answers
Bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA transcript. In this case, when lactose is required as a sugar source for the bacterium, the three genes of the lac operon can be expressed and their subsequent proteins translated: lacZ, lacY, and lacA. The gene product of lacZ is β-galactosidase which cleaves lactose, a disaccharide, into glucose and galactose. lacY encodes Beta-galactoside permease, a protein which becomes embedded in the cytoplasmic membrane to enable transport of lactose into the cell. Finally, lacA encodes β-galactoside transacetylase.
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At what levels of glucose and lactose should E. coli produce
galactoside permease and beta-galactosidase? (2...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
E. coli/brings lactose into its cell by using an enzyme called Lac Permease. Once the lactose...
E. coli/brings lactose into its cell by using an enzyme called Lac Permease. Once the lactose is inside, another enzyme called B-galactosidase converts the lactose into galactose and glucose. 1. How is E. coll's way of dealing with lactose similar to how your celis do it? 2. How is it different?
on strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of...
on strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. Tones where proteins bind promoter DNA genes and regulatory sequences 100 mm n galactosidase proteins B-galacto de branscetylave perman Lac A promoter Lacz Lac! Operator Lacy
A number of mutations affect the expression of the lactose operon in E. coli. Complete the...
A number of mutations affect the expression of the lactose operon in E. coli. Complete the table given below. Use a + to indicate that the enzyme is synthesized at greater than basal levels, and a 0 to indicate that the enzyme is not synthesized. Assume glucose is NOT present. Lactose Present B-galactosidase Permease Lactose Absent B-galactosidase Permease (a) I+O+Z+Y+ (b) 1 O+Z+Y+ (c) 1-O+Z+Y+ (d) I+0 Z+Y+ (e) I OʻZ+Y- (f) I+0 Z+Y-/ F'1-O+Z- Y+ (g) I+P+O+Z-Y+/ F' I+P-OZ+Y-...
You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity...
You are conducting an experiment using wild-type E. coli and you find that the beta-galactosidase activity of a culture grown in glycerol minimal medium is the same as a culture grown in lactose minimal medium. Is this expected? True or false: Glycerol is another possible carbon source for E. coli and it is not either glucose or lactose.
the answer I gave was wrong A mutant E. coli strain is found that synthesizes B-galactosidase...
the answer I gave was wrong
A mutant E. coli strain is found that synthesizes B-galactosidase whether or not lactose (or allolactose) is present. What mutations can independently lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences lac 1 operator promoter -35 lac z lac Y lac A JOSSA expression Lac repressor B-galactosidase B-galactoside- transacetylase proteins permease Lac Z Lac A promoter operator laci Lac Y
Lac Promoter i promoter galactoside transacetylase gene E coll chromosome lac operon loc terminator regulatory gene...
Lac Promoter i promoter galactoside transacetylase gene E coll chromosome lac operon loc terminator regulatory gene Operator site (laco) CAP site Lactose permease gene Beta-galactosidase gene
What happens to the expression of the Lac gene of E. coli if the following conditions...
What happens to the expression of the Lac gene of E. coli if the following conditions occur? For each condition below, mark an X in the column labelled Activated or Repressed, then explain why you chose that answer. Please assume the glucose levels are low in the cell. The first row is done for you. Activated a Repressed b Explain your answer Repressor, Lactose, and RNA polymerase are all present in abundance X Repressor binds to lactose so it can...
In E coli the lac operon will express enzymes for the digestion of (Glucose/Lactose) when it...
In E coli the lac operon will express enzymes for the digestion of (Glucose/Lactose) when it is present, but if both the sugar mentioned before and (Glucose/ Lactose) are present the lac operon is repressed, resulting in the (Glucose/ Lactose) being used first. If there are high levels of cAMP, this means (Glucose/Lactose) levels are low, this results in a (Represser/Promoter) being activated and (Decreasing/Increasing) transcription of the lac operon.
Background: Several genetic regions are needed for lactose metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene), lacY(permease gene), lacA(transacetylase...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
E. coli/brings lactose into its cell by using an enzyme called Lac Permease. Once the lactose is inside, another enzyme called B-galactosidase converts the lactose into galactose and glucose. 1. How is E. coll's way of dealing with lactose similar to how your celis do it? 2. How is it different?
on strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. Tones where proteins bind promoter DNA genes and regulatory sequences 100 mm n galactosidase proteins B-galacto de branscetylave perman Lac A promoter Lacz Lac! Operator Lacy
A number of mutations affect the expression of the lactose operon in E. coli. Complete the table given below. Use a + to indicate that the enzyme is synthesized at greater than basal levels, and a 0 to indicate that the enzyme is not synthesized. Assume glucose is NOT present. Lactose Present B-galactosidase Permease Lactose Absent B-galactosidase Permease (a) I+O+Z+Y+ (b) 1 O+Z+Y+ (c) 1-O+Z+Y+ (d) I+0 Z+Y+ (e) I OʻZ+Y- (f) I+0 Z+Y-/ F'1-O+Z- Y+ (g) I+P+O+Z-Y+/ F' I+P-OZ+Y-...
the answer I gave was wrong
A mutant E. coli strain is found that synthesizes B-galactosidase whether or not lactose (or allolactose) is present. What mutations can independently lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences lac 1 operator promoter -35 lac z lac Y lac A JOSSA expression Lac repressor B-galactosidase B-galactoside- transacetylase proteins permease Lac Z Lac A promoter operator laci Lac Y
Lac Promoter i promoter galactoside transacetylase gene E coll chromosome lac operon loc terminator regulatory gene Operator site (laco) CAP site Lactose permease gene Beta-galactosidase gene
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
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