An E. coli culture is grown in a variety of very rich media. With a number...
An E. coli culture is grown in a variety of very rich media. With a number of the media you are able to reduce the generation time to 20 mins. No further additions to the media reduce the doubling time below 20 mins. The supply of nutrients and building blocks are not limited. What factors could be limiting the growth rate of the cell?
Homework Answers
Though E.coli organisms are grown in enough media content, the metabolic byproduct of these microbes are inhibitors of their growth. So their doubling time could be limited due to this condition.
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An E. coli culture is grown in a variety of very rich
media. With a number...
The adaptation phase for the E. coli culture lasted A culture of E. coli was grown...
The adaptation phase for the E. coli culture lasted
A culture of E. coli was grown on suitable culture media; aliquots of the culture were serially diluted and spread plated aseptically on agar plate media at the times indicated in the table below. Upon overnight incubation at 37°C, colonies appeared and viable counts were colleted and displayed below. Hours 0 Viable Counts (CFU/mL) 2 .00 x 107 2.10 x 107 2.30 x 107 3.25 x 107 6.60 x 107 1.40...
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass...
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass of E. coli (in pounds) that will accumulate when a single cell of E. coli grows exponentially for 50 hours in these conditions, assuming that nutrients are not limiting. Show your work. (An E. Coli cell has a mass of 10-12gram/cell).
7. (10 points) E. Coli bacteria were grown in a 20 L batch cell culture reactor,...
7. (10 points) E. Coli bacteria were grown in a 20 L batch cell culture reactor, with the following data collected: Inoculation Time ---> Time 11:27 am 11:43 am 12:16 pm 1:19 pm 1:27 pm 1:42 pm 1:54 pm 2:05 pm 2:17 pm 2:41 pm Cell Count (per mL) 10,277,000 10,313,000 29,750,000 224,750,000 229,216,000 229,115,000 227,260,000 204,935,000 183,745,000 146,103,000 A. Draw a log10 X vs. time graph and label the phases for batch cell growth. B. Calculate the specific growth...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
Below is a table of results of E. coli grown in in nutrient broth (NB) and...
Below is a table of results of E. coli grown in in nutrient broth (NB) and minimal medium (MM) over 220 min. Time (min) A600 of NB MM 0 0.044 0.038 20 0.177 0.074 40 0.321 0.116 60 0.429 0.167 80 0.523 0.235 100 0.638 0.285 120 0.849 0.300 140 0.925 0.367 160 1.101 0.428 180 1.159 0.468 200 1.198 0.471 220 1.209 0.488 Using the above data, plot absorbance (log scale) versus time (linear), drawing both conditions on one...
"Three organisms were innoculated on a culture media. Results showed Escherichia coli as red colonies, no...
"Three organisms were innoculated on a culture media. Results showed Escherichia coli as red colonies, no growth of Staphylococcus epidermidis, and colorless colonies of Salmonella enterica. The medium is:" a selective and differential b differential c selective d minimal e heterotrophic "A bacterium was incubated with aeration in a nutrient medium containing two carbon sources. After logarithmic growth, a lag phase was observed for 25 minutes. Then logarithmic growth at a slower rate was observed. Which substate was preferred by...
Is a Culture of Entrepreneurial Problem Solving Key for India? India is a complex and chaotic place with hundreds of mil...
Is a Culture of Entrepreneurial Problem Solving Key for India? India is a complex and chaotic place with hundreds of millions of poor citizens and a ramshackle infrastructure—particularly when compared to the gleaming new ports, highways, and airports that China has built in recent decades. Moreover, India has long been plagued by a notoriously inefficient, albeit democratic, government. But these challenges and barriers have arguably produced something remarkable in India—a problem-solving mentality that helps many Indians to quickly, cheaply, and...
Title: Development of an Adhesion Assay and Characterization of an Adhesion-Deficient Mutant of Pseudomonas fluorescens RESULTS...
Title: Development of an Adhesion Assay and Characterization of an Adhesion-Deficient Mutant of Pseudomonas fluorescens RESULTS Bacterial adhesion to sand columns. In the initial column assays with soil-and-sand mixtures, P. fluorescens PfO-1 and E. coli SLH25 had the highest and lowest adhesion values, respectively. Therefore, the sand column assay was optimized to yield the greatest difference in percent adhesion for these two strains. PfO-1 bacteria grown in minimal medium to the logarithmic phase were washed and suspended in either minimal...
DISSONANCE BETWEEN EMPLOYEES Matt created his T-shirt business, T’d up, 30 years ago, building the company...
DISSONANCE BETWEEN EMPLOYEES Matt created his T-shirt business, T’d up, 30 years ago, building the company up from a small, no-name brand that he ran out of his parents’ garage while he was in school into a well-known local company that supplies custom T-shirts and other clothing and accessories for a wide variety of customers, both local and national. T’d up’s big break came 20 years ago when a local band became famous and sourced all of their concert clothing...
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DISSONANCE BETWEEN EMPLOYEES Matt created his T-shirt business, T'd up, 30 years ago, building the company up from a small, no-name brand that he ran out of his parents' garage while he was in school into a well-known local company that supplies custom T-shirts and other clothing and accessories for a wide variety of customers, both local and national. T'd up's big break came 20 years ago when a local band became famous and sourced all of their concert clothing...
The adaptation phase for the E. coli culture lasted
A culture of E. coli was grown on suitable culture media; aliquots of the culture were serially diluted and spread plated aseptically on agar plate media at the times indicated in the table below. Upon overnight incubation at 37°C, colonies appeared and viable counts were colleted and displayed below. Hours 0 Viable Counts (CFU/mL) 2 .00 x 107 2.10 x 107 2.30 x 107 3.25 x 107 6.60 x 107 1.40...
7. (10 points) E. Coli bacteria were grown in a 20 L batch cell culture reactor, with the following data collected: Inoculation Time ---> Time 11:27 am 11:43 am 12:16 pm 1:19 pm 1:27 pm 1:42 pm 1:54 pm 2:05 pm 2:17 pm 2:41 pm Cell Count (per mL) 10,277,000 10,313,000 29,750,000 224,750,000 229,216,000 229,115,000 227,260,000 204,935,000 183,745,000 146,103,000 A. Draw a log10 X vs. time graph and label the phases for batch cell growth. B. Calculate the specific growth...
6. Transposon mutagenesis was performed to identify genes that are involved in the biosynthesis of theA amino acid alanine. A wildtype culture of E. coli was infected with a defective phage carrying Tn10 A and the culture was plated on LB-tet media to obtain tet-resistant colony forming units. (LB is a rich growth media for E. coli and contains all amino acids required for bacterial growth) Individual CFU were then transferred to a multiwell plate containing liquid LB-tet media and...
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