The ethidium bromide stock solution concentration is 10g/L based on working concentration 10mg/ml
Based on gel electrophoresis we have to had working concentration will be 150ml * 0.5 ul=75ul we have to add and make up to final volume 150ml.
150*0.5 ul=75 ul
You have to add Ethidium Bromide to an agarose gel at 1µg/mL. The Ethidium Bromide stock...
To detect the DNA band in your gel, you need to add ethidium bromide (EtBr) from a concentrated stock solution (40 ug/ml) to get a final concentration of 5 ug/ml in your agarose solution. Unfortunately, you made your agarose solution (0.8% agarose and 50 ml volume) before adding the EtBr. Thus, when you add he appropriate volume of concentrated EtBr, you will increase the fila volume of agarose solution. a. What volume of the concentrated EtBr must you add to...
You currently have 20,000X ethidium bromide. You want to make 250 mL of 1X ethidium bromide dissolved in 1X TBE. You currently also have a stock of 10X TBE solution. How much 10X TBE do you need and how much 20,000X ethidium bromide do you need?
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
Can anyone show me step-by-step on how to do the agarose calculations? This week we will run the PCR reactions on an agarose gel and analyze the results. Safety notes: Ethidium bromide is a potent mutagen. Wear gloves when handling containers containing ethidium bromide, when handling gels that have been stained in ethidium bromide, and when working with the computer attached to the gel-doc system. Protocol I. Pouring agarose gels I. Using 10x TAE, prepare 50 ml of 3% (w/v)...
please hlep me answer those three questions asap. Please ignore question 7. Question 7 Working with buffers You need 100 ml of 120 mM phosphate buffer and you have two stock solutions from which you can make the buffer; 0.6 M NaH2PO4 (the acid form) and 0.6 M Na2HP04 (the base form). You need to add 3 times more of the base form than the acid form to achieve the desired pH. How will you make this solution? Question 8...
How much agarose would you need to prepare a 30 mL, 1.5% (w/v) agarose gel?
6. When performing agarose gel electrophoresis, how much 6X loading dye should you add to a 8 uL DNA sample before loading it onto the gel? (1 pt)
1A)The compound magnesium bromide is a strong electrolyte. Write the reaction when solid magnesium bromide is put into water: 1B)The compound zinc sulfate is a strong electrolyte. Write the reaction when solid zinc sulfate is put into water 1C)The compound cobalt(II) chloride is a strong electrolyte. Write the reaction when solid cobalt(II) chlorideis put into water: 1D)You need to make an aqueous solution of 0.171 M potassium iodide for an experiment in lab, using a 300 mL volumetric flask. How...
9,10,12 petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
In the laboratory you dissolve 19.8 g of calcium bromide in a volumetric flask and add water to a total volume of 500 ml. M. What is the molarity of the solution? What is the concentration of the calcium cation? м. What is the concentration of the bromide anion? In the laboratory you dissolve 19.8 g of calcium bromide in a volumetric flask and add water to a total volume of 500 ml. M. What is the molarity of the...