1.) You have isolated mitochondria and are testing various substrates by measuring the oxygen consumption with...
1.) You have isolated mitochondria and are testing various substrates by measuring the oxygen consumption with a special electrode. You add the inhibitor rotenone. What must be the substrate you add? Why?
Homework Answers
The substrate will be iron sulphur centre of complex 1. Rotenone is a chemical which blocks the transfer of electrons from iron sulphur centre of complex 1 to ubiquinone/coenzyme Q.
So, if we want to study the effect of rotenone, than the substrate will be iron sulphur centre of complex 1.
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1.) You have isolated mitochondria and are testing various
substrates by measuring the oxygen consumption with...
Question 1 (25 points). The mitochondria from a patient suffering from muscle pain during physical exercises...
Question 1 (25 points).
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
Part 1
The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a...
Question 1 The mitochondria from a patient suffering from muscle pain during physical exercises were analyzed...
Question 1
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
1) The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a healthy donor (control)....
1. You are studying an in vitro suspension of mitochondria that does not contain any NADH....
1. You are studying an in vitro suspension of mitochondria that does not contain any NADH. You run the experiment in a closed system with a set amount of oxygen; no new oxygen is introduced. (15pts) On the graph below, show the effect of adding fixed amounts of the following compounds at various times: • Time 1: ADP + Pi • Time 2: succinate (or any oxidizable substrate) • Time 3: oligomycin, an inhibitor of ATP synthase • Time 4:...
You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocate...
You have isolated a temperature-sensitive mutant that, at the restrictive temperature, has no ability to translocate proteins into mitochondria. So far you have eliminated any of the proteins forming the various transporters (TOM complex, SAM complex, TIM22 complex, TIM23 complex and OXA complex) and the porin proteins as candidates for the mutation. In other words, all the transporter proteins are wild type. What is your hypothesis for a protein that may be responsible for this mutant phenotype.
- I believed the inhibited protein was ATP synthase, however the data on Figure 2 does...
- I believed the inhibited protein was ATP synthase, however the
data on Figure 2 does not support this. Please help!
The mitochondria from a patient suffering from muscle pain during physical exercises were analyzed to detect any defect in one of the components of the respiratory chain. Isolated mitochondria were incubated in an isotonic buffer, and changes in the oxygen consumption were measured as various components were sequentially added to the reaction. 1) The following graph (Fig. 1) shows...
Table 1. Calculate the metabolic rates Respirometer Contents Oxygen consumption (ml O2/20 min) Oxygen consumption (ml...
Table 1. Calculate the metabolic rates Respirometer Contents Oxygen consumption (ml O2/20 min) Oxygen consumption (ml Oz/hr) A Germinating beans B Dry beans 1. What is the independent variable for the cellular respiration experiment? 2. What is the dependent variable for the cellular respiration experiment? 3. Explain why water moved into the respirometer 4. If you increase the temperature of the water bath that the respirometers were in, what would you predict would happen to the rate of cellular respiration...
PART A- Observe Normal Catalase Reaction To determine the volume of oxygen released in ml) during...
PART A- Observe Normal Catalase Reaction To determine the volume of oxygen released in ml) during the degradation of H202 by catalase, you will first do trials using the set-up in Fig. 1. 1. Place 10 ml of the 3% hydrogen peroxide solution into a clean bottle 2. Add quickly catalase containing solution. 3. What gas is being released? 4. Throughout this investigation you will estimate the rate of the reaction (by measuring the volume of water displaced by O2...
You have isolated the Na/K-ATPase in a semi-purified membrane system. Given higher concentrations of Na' out...
You have isolated the Na/K-ATPase in a semi-purified membrane system. Given higher concentrations of Na' out and higher K inside, addition of ATP to the inside leads to increases of Nat olutside and Kt inside as expected. If instead, you add the ATP to the outside of the membrane, you don't see any change in the ion levels. Why? • ATP acts as an irreversible inhibitor of the Na/K-ATPase if it binds on the exterior side. The active site for...
Draw the strcture of all substrates you will be using in this reaction and classify them...
Draw the strcture of all substrates you will be using in this
reaction and classify them as( primary, Secondary, and tertiary)
aryl bezylic or some combination of these (such as primary
bezylic). Identify the nucleophile, substrate, and leaving group in
general equations for reactions 1 and 2
ORRAS sminilah you dimensions (R) or (S), and show their stereochemistry clearly. If your instructor reguests, obtain another set of atoms and connectors and use it to prepare isomers as well. R eal...
1. A researcher suspects that consumption of some fruit berries found in the amazon basin may...
1. A researcher suspects that consumption of some fruit berries found in the amazon basin may have positive or negative effects on hemoglobin function. Can you predict what the effect of eating a significant amount of the berries would be on adult hemoglobin binding and distribution of oxygen if one of the phytochemicals in the berries has been found to be a competitive inhibitor of 2,3-Bisphosphoglycerate (2,3 BPG) (3 points). What do you think would be the effect of the...
Question 1 (25 points).
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
Part 1
The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a...
Question 1
The mitochondria from a patient suffering from muscle pain
during physical exercises were analyzed to detect any defect in one
of the components of the respiratory chain. Isolated mitochondria
were incubated in an isotonic buffer, and changes in the oxygen
consumption were measured as various components were sequentially
added to the reaction.
1) The following graph (Fig. 1) shows the changes of oxygen
concentration in a solution containing the patient’s mitochondria
or mitochondria from a healthy donor (control)....
1. You are studying an in vitro suspension of mitochondria that does not contain any NADH. You run the experiment in a closed system with a set amount of oxygen; no new oxygen is introduced. (15pts) On the graph below, show the effect of adding fixed amounts of the following compounds at various times: • Time 1: ADP + Pi • Time 2: succinate (or any oxidizable substrate) • Time 3: oligomycin, an inhibitor of ATP synthase • Time 4:...
- I believed the inhibited protein was ATP synthase, however the
data on Figure 2 does not support this. Please help!
The mitochondria from a patient suffering from muscle pain during physical exercises were analyzed to detect any defect in one of the components of the respiratory chain. Isolated mitochondria were incubated in an isotonic buffer, and changes in the oxygen consumption were measured as various components were sequentially added to the reaction. 1) The following graph (Fig. 1) shows...
Table 1. Calculate the metabolic rates Respirometer Contents Oxygen consumption (ml O2/20 min) Oxygen consumption (ml Oz/hr) A Germinating beans B Dry beans 1. What is the independent variable for the cellular respiration experiment? 2. What is the dependent variable for the cellular respiration experiment? 3. Explain why water moved into the respirometer 4. If you increase the temperature of the water bath that the respirometers were in, what would you predict would happen to the rate of cellular respiration...
PART A- Observe Normal Catalase Reaction To determine the volume of oxygen released in ml) during the degradation of H202 by catalase, you will first do trials using the set-up in Fig. 1. 1. Place 10 ml of the 3% hydrogen peroxide solution into a clean bottle 2. Add quickly catalase containing solution. 3. What gas is being released? 4. Throughout this investigation you will estimate the rate of the reaction (by measuring the volume of water displaced by O2...
You have isolated the Na/K-ATPase in a semi-purified membrane system. Given higher concentrations of Na' out and higher K inside, addition of ATP to the inside leads to increases of Nat olutside and Kt inside as expected. If instead, you add the ATP to the outside of the membrane, you don't see any change in the ion levels. Why? • ATP acts as an irreversible inhibitor of the Na/K-ATPase if it binds on the exterior side. The active site for...
Draw the strcture of all substrates you will be using in this
reaction and classify them as( primary, Secondary, and tertiary)
aryl bezylic or some combination of these (such as primary
bezylic). Identify the nucleophile, substrate, and leaving group in
general equations for reactions 1 and 2
ORRAS sminilah you dimensions (R) or (S), and show their stereochemistry clearly. If your instructor reguests, obtain another set of atoms and connectors and use it to prepare isomers as well. R eal...
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