Question

genetics.

I have part B. I am just confused on what it is asking in part A

DU V AAA Pane 3. (2pts) Interestingly, after identifying the region your mutation resides in, you discover that you have a nonsense mutation early on in a putative gene that has never been studied before! A quick structure prediction and protein homology domain search suggests this protein may be a transcription factor. You therefore name it ATF1 for Aging-related Transcription Factor. To identify what genes ATF1 regulates, vou convince your adviser to spend the money to perform RNAseg on your WT and ATF1 mutant cells. The data obtained are shown in the graph below. Name: BIOL302 S20 Smith Problem Set #3 a. Which RNA(s) if any can be used as a control to make sure your RNAseg, experiment worked? Why? AGAT ARPA ASCI ATF1 Plot Area b. Which RNA(s) If any have been affected by mutating ATF1? For any identified list if they have been upregulated (more RNA) or downregulated (less RNA) in mutant cells. MME4 MNMI NEM

Answer 1

RNA levels of ATF1 could be used as the negative control here. As you are comparing differential RNA expression between Control and ATF1 mutant cell here, RNA levels of ATF1 are nil (which means that ATF1 null cells do not have ATF1 mRNA), hence you could use it as a negative control reference.