Question

can someone help explain part b

4. Transcription. The DNA below contains a promoter sequence recognized by E. coli RNA polymerase (KNAP): 5'-AACGTAACTGAATTCCGCAATGGCATGGCATTGCTCATTATACTTAGTCTAATATGTCAA-3 3'-TTGCATTGACTTAAGGCGTTACCGTACCGTAACGAGTAATATGAATCAGATTATACAGTT-5'. A) B) Draw boxes around the two promoter elements, centered at -10 and -35. relative to the start site of transcription Transcription starts at the A-T base pair, which is indicated by the bold letters in the DNA shown above. Based on the asymmetric promoter sequence, RNAP selects one strand as the template for RNA synthesis. Draw the structures of (i) the first two substrates used by RNAP, and (ii) the dinucleotide product and pyrophosphate, byproduct. [You can use stick representations for carbons.)

Answer 1

5'-AACGTAACTGAATTCCGCAATGGCATGGCATTGCTCATTATACTTAGTCTAATATGTCAA-3' 3'-TTGCATTGACTTAAGGCGTTACCGTACCGTAACGAGTAATATGAATCAGATTATACAGTT-5' -10 promoter -35 promoter RNA polymerase recognition promoter sequences of -10 (TATAAT) and -35 (TTGACA) in the 5' to 3' direction by the sigma subunit of RNA polymerase

Nth RNA O--0-20 RNA-osa -o-pogacth Nih L2-0 Ma osa- -p-Cho N OR CH OH OH to on Growing RNA + Nucleotide monophaishare Growing RNA Nucleotide tri-phosphate or o o Pyrophoplate-PP: