In the trp operon could Tryptophan get synthesized if the bacterial DNA was mutated at the site of the Promoter sequences? In other words, if the Promoter couldn’t function normally would Tryptophan be synthesized? Would you get a different answer depending on whether or not there was Tryptophan present in the cell? Give a molecular explanation.
Ans: Trp operon is involved in anabolic process of producing tryptophan and tryptophan is acting as a repressor molecule for this operon that means if the cell have enough amount of tryptophan then this operon switches off and vice-versa. Now coming to the question if the bacterial DNA is mutated at the site of the promoter sequence and it causes RNA polymerase to not able to bind with the promoter sequence and this will stop the transcription initiation of the genes involved in the anabolic pathway of tryptophan production and so the production of tryptophan because if the genes are not able to express than the substrate will not be converted into tryptophan.
When the cell has enough amount of tryptophan then the trp bound to the repressor changing the repressor to its active site, this active repressor attaches to the operator, blocking RNA polymerase from binding to the promoter and preventing transcription of the operon. So the concentration of tryptophan is only linked with the activation of repressor and not with the promoter mutation so we will get same answer whether or not there was tryptophan present in the cell.
In the trp operon could Tryptophan get synthesized if the bacterial DNA was mutated at the...
In the ara operon could Arabinose be metabolized if the Transcription Factor was mutated so it could not bind to the Inducer? In other words, if the Inducer couldn’t bind to the ara Transcription Factor would Arabinose still get broken down? Would you get a different answer depending on whether or not there was Arabinose present in the cell? Give a molecular explanation.
8. In transcription, A) mRNA is synthesized from DNA B) the starting point of synthesis is the promoter site C) RNA polymerase binds to the promoter during initiation D) the ending point of synthesis is the terminator site E) All of the above The tryptophan (trp) operon is actively transcribed (i.e. gene expression occurs) A) when there is excess tryptophan in the cell. B) when there is a lack of tryptophan in the cell. C) constitutively because the trp operon...
Page < 2 > of 2 D. The trp operon would be expressed at high levels, comparable to its expression in the absence of trp 6. Based upon what you have learned about the E. coli trp operon, what could you say about expression of its structural genes in cells with large amounts of environmental trp, but expressing a mutant trp repressor that is unable to bind trp? A. The trp operon would be completely repressed due to the abundance...
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A genetically engineered plasmid Ficarries all of the elements of the lac operon, but replaces the structural genes with the reporter gene green fluorescent protein (GFP). The repressor allele cannot bind lactose, and the OC allele cannot be bound by the repressor. Given the following genotypes and growth media that does not contain glucose, indicate in the table below whether you expect to see b-galactosidase activity and glowing green colonies of E. coli by selecting Yes or No. growth media...
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