It is common to use 25 ng of DNA for a sequencing reaction. You have a...
- It is common to use 25 ng of DNA for a sequencing reaction. You have a sample that has an absorbance reading of 0.025 after being diluted 1:200. What volume of the original DNA should you use? (Show math for partial credit.)
Homework Answers
Concentration =
Absorbanve/Pathlength×Conversion factor×Dilution factor
= 0.025/1 *50 ng/ul* 200 = 250 ng/ul
Original DNA sample has 250 ng/ul and we need only 25ng hence we can dilute 1:10 ratio and use 1 ul of diluted sample that contains 25ng/ul for sequencing
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