Question

need help with 3-5

3) What is the basis for the difference in electrophoretic mobility between normal and sickle cell beta globin proteins? 4) How many individual peptide strands is hemoglobin composed off 5) What is the change in the total charge between the HbA and HbS?

Answer 1

3. What is the basis basis for for the in electrophoretce difference mobi the obility between normal eta globin Proteins? and sickle e a cell and sickle cell o move not suver. When a normal cell and a sig hemoglobin is run in the gel electrophores both the moleentes migrate to towards positive pole fased on their shape But the sickle cell hemoglobina SLOWER as compared to normal hemogloben. is because of its SICKLE SHAPE - HB HBS will migrate slower than H&A because it lacks the negative charge gluta This can only be done once Has is being banslated 4. How many individual peptide shands in is hemoglobin composed of ? Answer FOUR The hemoglobin molecule is made up of 4 polypeptide chains two alpha chain of 141 amino acid residues each and two pela chain on 146 a residue each The d and B chain have different sepnences of aã, but fold up to form similar 3D structures.

5. What is the change in the total charge between the HbA and HbS? - Ans. - In both cases, sickle cell hemoglobin PH65) is shown at pH 86 with a met charge which is three cenits more positive than the norma hemogloben HEA - In one case, however, this than #in the result of Three more positive charses on HES than on HOA
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