A blank is meant to be used as a control for the spectrophotometric experiments.
Water is used in most cases since water as the common solvent used in dissolving the chemical reagents or the biomolecules.in order to measure their concentration.
A complex mixture is used when the mixture itself is used as a solvent for dissolving component whose concentration has to be determined.
1. In today's experiment, tube 1 represented the blank. Why did we use a complex mixture...
2. The tube wrapped in aluminum foil served as a control in today's experiment. What type of control was it and why? (3 points)
1. A TLC experiment is performed to separate a mixture of the compounds shown below. Label each chemical with the appropriate letter based off the TLC data. (2 points) • B OCH 2. What would happen to the Rivalues for your compounds if 30:70 hexane:acetone were used as the developing solvent instead of 70:30 hexane:acetone? Briefly explain why. (2 points) 3. After first using 90:10 hexane:acetone as the solvent, we switched to 70:30 hexane:acetone. Why did we not use 70:30...
Organic Chemistry Lab question In the experiment we did acid-base extractions to find impurities. The impurity was either benzoic acid (acid) or 4-chloroaniline (base) So in this experiment we did Acid-Base extraction with an unknown compound. So we had an unknown of 0.150 grams and mixed it with 20 mL of diethyl ether. We placed this in a separatory funnel and added 2mL of 1.0 NaOH solution to the separatory funnel and placed the stopper over the top of the...
These questions are from an experiment where we had a mixture of Ferrocene, acetylferrocene and diacetyl ferrocene and we separated each by using column chromatography. Before doing the column chromatography we did TLC analysis to ensure that we did, in fact, have a mixture of these 3 compounds. During the column chromatography we first eluted the ferrocene using pure hexane. Then we eluted the acetylferrocene using a 1:1 mixture of hexane and methylene chloride. Then we eluted the diacetylferrocene using...
Reactants Color of the mixture after adding Biuret reagent Tube 1 (egg white + pepsinogen + NaOH) Blue Tube 2 (egg white+ pepsinogen + HCl) Purple Tube 3 (egg white + NaOH) Blue Tube 4 (egg white + HCl) Blue a. In which tube(s) did protein digestion occur? How do you know? b. In which tube(s) protein digestion did not occur? How do you know? c. What effect does pH have on protein digestion with pepsin? Why? 3. Reactants Color...
Microscale techniques: In today's experiment we will be using very small amounts of chemical reactants. This method is generally less expensive and more environmentally-friendly than traditional "test tube" chemistry. Some of the waste products produced in today's experiment, particularly those containing lead and barium, are harmful to the environment. By using such small amounts of chemical reactants we generate far less waste than by using larger scale methods. Procedure 1. Put on your safety goggles. Be sure to wear them...
greenhouse on Mars .. no dayes provied 1. Why oh Why did we use substandard shielding on our Martian greenhouse? Please explain the effects of the following mutations and justify your answer for each (50 pts total, 5 pts each) a. The water splitting complex of PSIl works at about half the rate it did previously b. The number of antenna molecules in the photosystems (chlorophyll, carotenoids) has been decreased by 80% but the special pair is intact. c. Cytb6/f...
In melting point experiment, (we use benzoic acid (solid),acetanilide (solid) and we put them in capillary tube and let them heat with thiele tube.) why Raoult's law is related to melting point? I think Raoult's law is only in liquid. please make me understand.. How solids are interact with each other as liquid?
For each experiment described below 1) provide the relevant equilibrium reaction or reactions, 2) use LeChatelier's Principle to explain why the reaction has shifted left (L) or right (R), and 3) answer any other questions. a) To a solution containing 1M Pb(NO3)2, 1 M HCl is slowly added until a white precipitate forms. The mixture is then heated and the precipitate disappears. Is the dissociation of the precipitate endothermic or exothermic? Does the equilibrium constant (Ksp) change when the solution...
1) Why do we use a mixture of ethyl acetate and hexanes as solvents in the synthesis of 5,6-endo-dicarboxylic anhydride? 2) What do the terms exo and endo refer to? 3) Why do we not do any TLC on the product in the synthesis of 5,6-endo-dicarboxylic anhydride?