Homework Answers
The above species is a spore former because there are two stains staining the endospre and the vegetative cell.Schaeffer-Fulton method can be used to distinguish spore and non spore forming bacteria which uses Malachite green which will stain the endospore green. As we know that endospore is hard and impervious to reagents therefore it is either given heat treatment or detergent treatment and then only it can enterthe spore. Then it is treated with alcohol which will remove the stain from the vegetative cells and then the vegetative cells are counterstained with saffranin and stained pink.
The spore formation can also be detected by carbofuschin reagent which uses similar mechanism here endospore are stained red and the vegetative cell are stained with methylene blue and stained blue.
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Here is a spore stain on an unknown (pure) culture. It this species a spore former...
After performing a Gram stain on a pure culture of bacteria, you observe a clumpy cellular...
After performing a Gram stain on a pure culture of bacteria, you observe a clumpy cellular arrangement that looks like irregular grape clusters. Which of the following statement(s) are correct? a.the bacteria are Gram positive b.the division planes are not at right angles to one another c. the bacteria are Staphylococci d. the bacteria only divide along the traverse plane e. the bacteria are Streptococci
A Gram stain of unknown bacterium A grown for 24 hours show purple cells. Is bacterium...
A Gram stain of unknown bacterium A grown for 24 hours show purple cells. Is bacterium A Gram positive or Gram negative? A gram stain of bacterium A grown for 72 hours shows both purple and pink stained cells. Additional analysis shows that bacterium A is NOT contaminated. Explain these results. You are given 2 nutrient broth tubes each containing a pure culture of one of two Gram positive bacteria. Unfortunately, the labels have washed off and you are asked...
WORKSHEET Hemolytic activity Gram Stain Cell Arrangement Blood agar plates: Bacterial Species S. pyogenes E. faecalis...
WORKSHEET Hemolytic activity Gram Stain Cell Arrangement Blood agar plates: Bacterial Species S. pyogenes E. faecalis S. pneumoniae + + + QUESTIONS 1. List the disease caused by beta-hemolytic streptococci. 2. List the areas of the body where streptococci are indigenous. 3. What types of hemolysis occurred for each of the species of Streptococcus you tested? 4. What types of hemolysis occurred on the blood agar plate containing your throat culture 5. Were you able to identify the type of...
Question 1 15 pts USING THE PROVIDED INFORMATION, DETERMINE THE IDENTITY OF THE UNKNOWN BACTERIA BASED...
Question 1 15 pts USING THE PROVIDED INFORMATION, DETERMINE THE IDENTITY OF THE UNKNOWN BACTERIA BASED UPON THE FIVE SPECIES UTILIZED IN THE LABORATORY THIS SESSION AND ANSWER THE PROVIDED QUESTIONS GRAM'S STAIN RESULT UNKNOWN GRAM'S STAIN ACID-FAST STAIN RESULT UNKNOWN VL ACID FAST STAIN ACID-FAST STAIN RESULT UNKNOWN #1 ACID-FAST STAIN REACTION CATALASE TEST RESULT UNKNOWN 21 CATALASE REACTION 1 What is the identity of your unknow sample based on the results seen in the above images? What is...
Laboratory Report 9 Pure Culture Techniques A. Results 1. Evaluation of Streak Plate Show within the...
Laboratory Report 9 Pure Culture Techniques A. Results 1. Evaluation of Streak Plate Show within the circle the distribution of the colonies on your streak plate. To identify the colonies, use red for Serratia marcescens and yellow for Micrococcus luteus. (Use purple for Chromebacterium violaceumi and yellow for E. coli if these species were included in your mixed culture.) If time permits, your instructor may inspect your plate and enter a grade where indicated. Grade 2 Evaluation of Pour Plates...
1. The graph below depicts the growth of a Gram-positive, spore-forming bacterium Bacillus thuringiensis (B. t.). This species normally lives in the soil as well as the alkaline gut of insects. a)...
1. The graph below depicts the growth of a
Gram-positive, spore-forming bacterium Bacillus thuringiensis (B.
t.). This species normally lives in the soil as well as the
alkaline gut of insects.
a) Label on the graph the phases of this growth cycle.
Below, describe the important features of each phase.
b) The graph shown was obtained by culturing B. t. in
media containing only minimal amounts of nutrients and salts. Draw
a growth curve on the same graph that would...
mixed unknown. the goal of this assignment is to identity each organism. so this attachement or...
mixed unknown. the goal of this assignment is to identity each
organism. so this attachement or picture has both organism
seperated by gram stanin and result of each on macConkeys Agar and
Columbia CNA. so in order to identify the genus and specise both
organsims, i have to order 2 additional test per. MY questions to
you is what kinds of test do have to requesd or do in oder to
identity the organism ?
N (No Title) Gram + Gram...
The salinity of seawater is mainly due to the presence of KCl. The normal freezing point of seawater is -2.2 oC. Diluted seawater is used to culture fertilized eggs of a specific species of fish. The osmotic pressure of the fish eggs against pure water i
The salinity of seawater is mainly due to the presence of KCl. The normal freezing point of seawater is -2.2 oC. Diluted seawater is used to culture fertilized eggs of a specific species of fish. The osmotic pressure of the fish eggs against pure water is 14 bars at 15 oC. Calculate the volume (in L) of seawater you need to make 2 liters of isotonic solution for the fish eggs (the culturing condition is 15 oC, 1 bar).
+ Mock Practical 1-1. Read-Only Drew Design Transitions Animations Slide Show ANA des Section BTYS -...
+ Mock Practical 1-1. Read-Only Drew Design Transitions Animations Slide Show ANA des Section BTYS - A L -A- View Rev E Help . Tutenda Dube TD ser x - comments File Shape Arrange Our Shape Outline - - s pect Paragno Sale Q: 3-5 3&4 What type of microorganism is the one under the microscope How can you tell A Note ofte og 105 AM mere to search 2A Helo Share Comments ayout - AAEEEEE. A-2A-FEE e Find 2...
Spectroscopy Unknown. The spectra and data provided were obtained from a pure organic molecule. F...
Spectroscopy Unknown
Spectroscopy Unknown. The spectra and data provided were obtained from a pure organic molecule. For iH NMR Spectra, the integral is given in number of hydrogens (th) or as a relative ratio. Important coupling constants (J-values) are listed next to the peaks for some examples. For some spectra, an inset (grey box) is also given showing a "zoom-in" on an important part of the spectrum. Submit your answer for Quiz 8 at: http://go.osu.edu/CH2550QUIZ Mass Spectrum (not shown): [M-223...
A Gram stain of unknown bacterium A grown for 24 hours show purple cells. Is bacterium A Gram positive or Gram negative? A gram stain of bacterium A grown for 72 hours shows both purple and pink stained cells. Additional analysis shows that bacterium A is NOT contaminated. Explain these results. You are given 2 nutrient broth tubes each containing a pure culture of one of two Gram positive bacteria. Unfortunately, the labels have washed off and you are asked...
WORKSHEET Hemolytic activity Gram Stain Cell Arrangement Blood agar plates: Bacterial Species S. pyogenes E. faecalis S. pneumoniae + + + QUESTIONS 1. List the disease caused by beta-hemolytic streptococci. 2. List the areas of the body where streptococci are indigenous. 3. What types of hemolysis occurred for each of the species of Streptococcus you tested? 4. What types of hemolysis occurred on the blood agar plate containing your throat culture 5. Were you able to identify the type of...
Question 1 15 pts USING THE PROVIDED INFORMATION, DETERMINE THE IDENTITY OF THE UNKNOWN BACTERIA BASED UPON THE FIVE SPECIES UTILIZED IN THE LABORATORY THIS SESSION AND ANSWER THE PROVIDED QUESTIONS GRAM'S STAIN RESULT UNKNOWN GRAM'S STAIN ACID-FAST STAIN RESULT UNKNOWN VL ACID FAST STAIN ACID-FAST STAIN RESULT UNKNOWN #1 ACID-FAST STAIN REACTION CATALASE TEST RESULT UNKNOWN 21 CATALASE REACTION 1 What is the identity of your unknow sample based on the results seen in the above images? What is...
Laboratory Report 9 Pure Culture Techniques A. Results 1. Evaluation of Streak Plate Show within the circle the distribution of the colonies on your streak plate. To identify the colonies, use red for Serratia marcescens and yellow for Micrococcus luteus. (Use purple for Chromebacterium violaceumi and yellow for E. coli if these species were included in your mixed culture.) If time permits, your instructor may inspect your plate and enter a grade where indicated. Grade 2 Evaluation of Pour Plates...
1. The graph below depicts the growth of a
Gram-positive, spore-forming bacterium Bacillus thuringiensis (B.
t.). This species normally lives in the soil as well as the
alkaline gut of insects.
a) Label on the graph the phases of this growth cycle.
Below, describe the important features of each phase.
b) The graph shown was obtained by culturing B. t. in
media containing only minimal amounts of nutrients and salts. Draw
a growth curve on the same graph that would...
mixed unknown. the goal of this assignment is to identity each
organism. so this attachement or picture has both organism
seperated by gram stanin and result of each on macConkeys Agar and
Columbia CNA. so in order to identify the genus and specise both
organsims, i have to order 2 additional test per. MY questions to
you is what kinds of test do have to requesd or do in oder to
identity the organism ?
N (No Title) Gram + Gram...
+ Mock Practical 1-1. Read-Only Drew Design Transitions Animations Slide Show ANA des Section BTYS - A L -A- View Rev E Help . Tutenda Dube TD ser x - comments File Shape Arrange Our Shape Outline - - s pect Paragno Sale Q: 3-5 3&4 What type of microorganism is the one under the microscope How can you tell A Note ofte og 105 AM mere to search 2A Helo Share Comments ayout - AAEEEEE. A-2A-FEE e Find 2...
Spectroscopy Unknown
Spectroscopy Unknown. The spectra and data provided were obtained from a pure organic molecule. For iH NMR Spectra, the integral is given in number of hydrogens (th) or as a relative ratio. Important coupling constants (J-values) are listed next to the peaks for some examples. For some spectra, an inset (grey box) is also given showing a "zoom-in" on an important part of the spectrum. Submit your answer for Quiz 8 at: http://go.osu.edu/CH2550QUIZ Mass Spectrum (not shown): [M-223...
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