If Dam methylase is overproduced in E. coli would you expect it to have any effect...
Homework Answers
Overproduction of Dam methylase in E. Coli leads to an increased mutation rate because newly synthesized DNA becomes methylated before it can be correted and thus it leads to mutation in chromosome.
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If
Dam methylase is overproduced in E. coli would you expect it to
have any effect...
1. On which plate(s) would you expect to find bacteria most like the E. coli on...
1. On which plate(s) would you expect to find bacteria most like the E. coli on the source plate? Explain. 2. On which plate(s) would you find only genetically transformed bacterial cells? Why? 3. What is the purpose of the control plates? Explain the difference between the controls and why each one is necessary. 4. Why would one compare the -DNA/+Amp and DNA/+Amp plates?
In order to maximize production of a human enzyme by E. coli during cloning, would you...
In order to maximize production of a human enzyme by E. coli during cloning, would you use an E.coli promoter or human promoter to synthesize the enzyme mRNA in E. coli cells? Briefly, why?
QUESTION 2 What qualitative effect would you expect each of the following to have on the...
QUESTION 2 What qualitative effect would you expect each of the following to have on the Pso of hemoglobin? Explain. a. Increase in pH from 7.2 to 7.4 b. Increase in Pco, from 20 to 40 mm Hg c. Dissociation into monomer polypeptide chains d. Decrease in 2,3-BPG concentration from 7 mM to 5 mM in red cells.
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and...
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and its growth conditions. Indicate whether you would expect the rate of β-galactosidase synthesis to increase, decrease, or remain the same after each of the following changes to the growth medium. Explain your answer. a. E. coli cells are cultured in minimal medium containing glycerol as the sole b. E. coli cells are cultured in minimal medium containing glucose as the sole c. E. coli...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts...
You have systematically mutagenized the lac operon in E. coli to produce a mutation that disrupts the function of each of the following elements: a. the promoter for LacI (P(I)) b. the LacI gene c. CRP binding site d. the promoter for the lac operon (P(lac)) e. the operator sequence f. a mutation in lacZ that disrupts the coding region but does not disrupt transcription g. a mutation in lacZ that blocks transcription For each of the above mutations, what...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
Would you expect an amorphous piece of solid polyethylene (PE) to be isotropic or anisotropic? Briefly...
Would you expect an amorphous piece of solid polyethylene (PE) to be isotropic or anisotropic? Briefly explain............(2 points) Briefly explain why, upon solidification, an alloy of eutectic composition forms a microstructure consisting of alternating layers of the two solid phases. What is this structure called? What kind of effect does this structure have on the melting point of the alloy and why? (5 points)
1) Describe the similarities and differences between the lac operon in E. coli and the GAL...
1) Describe the similarities and differences between the lac operon in E. coli and the GAL system in yeast. 2) What effect does mutation at nucleotides within a silencer element have on the expression of the gene associated with the silencer? Why does it have this effect?
In what brain structure would you expect cocaine to have the largest effect on dopamine transmission...
In what brain structure would you expect cocaine to have the largest effect on dopamine transmission and why?
4. You have prepared a batch of competent E coli cells and found its transformation efficiency...
4. You have prepared a batch of competent E coli cells and found its transformation efficiency is 106 per μg plasmid DNA. A professional scientists prepared competent E. coli cells having a transformation efficiency of about 108 cells/ g plasmid DNA Calculate % score of your E. coli transformation efficiency (Τ.Ε.).
1. On which plate(s) would you expect to find bacteria most like the E. coli on the source plate? Explain. 2. On which plate(s) would you find only genetically transformed bacterial cells? Why? 3. What is the purpose of the control plates? Explain the difference between the controls and why each one is necessary. 4. Why would one compare the -DNA/+Amp and DNA/+Amp plates?
QUESTION 2 What qualitative effect would you expect each of the following to have on the Pso of hemoglobin? Explain. a. Increase in pH from 7.2 to 7.4 b. Increase in Pco, from 20 to 40 mm Hg c. Dissociation into monomer polypeptide chains d. Decrease in 2,3-BPG concentration from 7 mM to 5 mM in red cells.
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and its growth conditions. Indicate whether you would expect the rate of β-galactosidase synthesis to increase, decrease, or remain the same after each of the following changes to the growth medium. Explain your answer. a. E. coli cells are cultured in minimal medium containing glycerol as the sole b. E. coli cells are cultured in minimal medium containing glucose as the sole c. E. coli...
2a. (4 pts) A potent mutagen was added to an E. coli culture growing in rich broth. Prior experiments established that this F cell line, designated WWU113, could not synthesize leucine. You are interested in measuring the rate of induced reverse mutation. You plan to grow a liquid culture of the leu strain and then plate various dilutions of the culture onto selective media. What media should you use to grow up the liquid culture? This will be Media A...
Would you expect an amorphous piece of solid polyethylene (PE) to be isotropic or anisotropic? Briefly explain............(2 points) Briefly explain why, upon solidification, an alloy of eutectic composition forms a microstructure consisting of alternating layers of the two solid phases. What is this structure called? What kind of effect does this structure have on the melting point of the alloy and why? (5 points)
4. You have prepared a batch of competent E coli cells and found its transformation efficiency is 106 per μg plasmid DNA. A professional scientists prepared competent E. coli cells having a transformation efficiency of about 108 cells/ g plasmid DNA Calculate % score of your E. coli transformation efficiency (Τ.Ε.).
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