Homework Answers
Ans- The given image is of ELISA, sandwich ELISA.
Explanation- This is so because the antigen is seen sandwiched between primary and secondary antibody. The enzyme attached to secondary antibody.
Labelling-
1- primary antibody
2-antigen
3-secondary antibody
4- enzyme substrate
5- colored product
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ELISA or sandwhich or a different one?
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You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a...
You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice? A. Shelf life of reagents B. Speed of detection C. Wavelengths detected by microtiter plate reader D. Sensitivity Which assay format is the best choice for measuring small molecules, such as drugs and small peptide hormones? A....
The purpose of using two different protein-specific monoclonal antibodies in ELISA is to Choose one: O...
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What type of Fourier analysis technique can be used to analyze the signal in Figure 2?...
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4. This ELISA method coats a 96 well plate with the antigen cocaine. If the patient's...
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The purpose of using two different protein-specific monoclonal antibodies in ELISA is to Choose one: O A. provide multiple binding spots for the enzyme-linked signaling antibody O B. improve detection by having two different detection antibodies. O C. improve specificity by forming a "sandwich" with the antigen protein in the middle. OD. Improve detection by having two different immobilized capture antibodies
ELISA 1. This ELISA method coats a 96 well plate with the antigen opium. If the patient's serum contains antibodies for opium, their primary antibody will bind to the antigen. Then a secondary conjugated antibody is used to bind to the primary antibody. What specific testing method, utilizes two types of antibodies? 2. Which ELISA method coats a 96 well plate with the antibodies that target THC? If the patient's serum contains the antigen THC, then they will test positive...
What type of Fourier analysis technique can be used to analyze the signal in Figure 2? Justify your answer. 0 -5 -10 -15 Figure 2 X-20 -25 -30 -35 -40 0 0.5 1.5 х
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
4. This ELISA method coats a 96 well plate with the antigen cocaine. If the patient's serum contains antibodies for cocaine, their primary antibody will bind to the antigen, therefore, detected the drug. What specific testing method, utilizes only one type of antibody in this describe procedure?
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