Homework Answers
1. This is an example of indirect ELISA. In indirect ELISA antigen is coated on the wells and the antibody present in the sample binds to it and then a secondary antibody is added which is specific to the primary antibody. In this, the secondary antibody is specific to the primary antibody. This is called indirect ELISA.
2.
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ELISA 1. This ELISA method coats a 96 well plate with the antigen opium. If the...
4. This ELISA method coats a 96 well plate with the antigen cocaine. If the patient's...
4. This ELISA method coats a 96 well plate with the antigen cocaine. If the patient's serum contains antibodies for cocaine, their primary antibody will bind to the antigen, therefore, detected the drug. What specific testing method, utilizes only one type of antibody in this describe procedure?
pls help, ty ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
Question 12 parts a,b,c Q12. Using the information provided below on ELISA, answer the following questions...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
QUESTION 1 What is a heterophile antigen? a. an antigen homospecific to only one antibody. b....
QUESTION 1 What is a heterophile antigen? a. an antigen homospecific to only one antibody. b. an antigen that has been modified to bind to more than one antibody. c. an antigen found only on one etiological agent. d. an antigen common to more than one species and whose distribution is unrelated to its phylogenetic distribution. 1 points QUESTION 2 What is an antigen? a protein synthesized and secreted by T lymphocytes (T cells). All bacterial cells are antigens....
For questions 51 - 55 use the descriptions from the list below to label the diagnostic...
For questions 51 - 55 use the descriptions from the list below to label the diagnostic test diagram: Indirect ELISA 55. 53. 54.4 - A51: - A52: -A53: - 54: - A55: A. added enzyme substrate is converted to a product which can be measured via spectrophotometry B. pathogen antigen is attached to well in plate C. enzyme-labeled anti-antibodies are added and bind to bound antibodies D. patient serum is added; if present, complementary antibody binds to antigen E. after...
please help thanks! 1. Antibodies that are produced in an individual in response to antigen X...
please help thanks!
1. Antibodies that are produced in an individual in response to antigen X from species Y but are able to bind to an unrelated antigen A from species B are referred to as: a. cold agglutinins b. heterophile agglutinins c. auto agglutinins d. febrile agglutinins 2. The Rheumatoid Factor is primarily an IgM immunoglobulin against the patient's own: a. Fc mu region b. Fc gamma region c. Fab gamma region d. Fab mu region 3. Clumping and...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
please help solve In a direct ELISA test, the method is determining if by attempting to...
please help solve
In a direct ELISA test, the method is determining if by attempting to bind a labeled antibody to it. is/are present in the patient's sample Select one: O a. antibodies against a pathogen found in a patient's blood. ob,an antigen from a particular pathogen. O ca known sequence of DNA from a pathogen. d. a bacteriophage that can infect a particular bacterial strain Protozoan motility structures include: Select one: O a. cilia, flagella, and pseudopods. O b....
! Body Text ListParagraph No Spacing Table Paragr. Heading 1 Mary B., a 30-year-old woman with...
! Body Text ListParagraph No Spacing Table Paragr. Heading 1 Mary B., a 30-year-old woman with a history of intravenous (IV) drug use, was seen at a health clinic with complaints of swollen glands, fatigue, fever, weight loss, fre- quent vaginal yeast infections, and a persistent skin rash that had not responded to treatment. The physician ordered the laboratory tests shown in Tables 4...5 to 4.7. is Table 4-5 COMPLETE BLOOD COUNT Mary B. WBC RBC Hb Het MCV MCH...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing...
Can you please explain in simple terms the methods of this experiment? I'm having difficulty visualizing and understanding what is being done. I have copied and pasted some of the text from the article. Thank you! Abstract : Obesity, high-fat diets, and subsequent type 2 diabetes (T2DM) are associated with cognitive impairment. Moreover, T2DM increases the risk of Alzheimer's disease (AD) and leads to abnormal elevation of brain beta-amyloid levels, one of the hallmarks of AD. The psychoactive alkaloid caffeine...
4. This ELISA method coats a 96 well plate with the antigen cocaine. If the patient's serum contains antibodies for cocaine, their primary antibody will bind to the antigen, therefore, detected the drug. What specific testing method, utilizes only one type of antibody in this describe procedure?
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
Question 12 parts a,b,c
Q12. Using the information provided below on ELISA, answer the following questions system to recognize an infectious and in Background While antibodies are used by the immune system to recognize scientists also use antibodies for a myriad of laboratory and the body, scientists also use antibodies for a agent in assays. If a scientist aims to identify a specific protein or mal y and clinical heterogeneous mixture such as a cell lysate or blood e such...
For questions 51 - 55 use the descriptions from the list below to label the diagnostic test diagram: Indirect ELISA 55. 53. 54.4 - A51: - A52: -A53: - 54: - A55: A. added enzyme substrate is converted to a product which can be measured via spectrophotometry B. pathogen antigen is attached to well in plate C. enzyme-labeled anti-antibodies are added and bind to bound antibodies D. patient serum is added; if present, complementary antibody binds to antigen E. after...
please help thanks!
1. Antibodies that are produced in an individual in response to antigen X from species Y but are able to bind to an unrelated antigen A from species B are referred to as: a. cold agglutinins b. heterophile agglutinins c. auto agglutinins d. febrile agglutinins 2. The Rheumatoid Factor is primarily an IgM immunoglobulin against the patient's own: a. Fc mu region b. Fc gamma region c. Fab gamma region d. Fab mu region 3. Clumping and...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
please help solve
In a direct ELISA test, the method is determining if by attempting to bind a labeled antibody to it. is/are present in the patient's sample Select one: O a. antibodies against a pathogen found in a patient's blood. ob,an antigen from a particular pathogen. O ca known sequence of DNA from a pathogen. d. a bacteriophage that can infect a particular bacterial strain Protozoan motility structures include: Select one: O a. cilia, flagella, and pseudopods. O b....
! Body Text ListParagraph No Spacing Table Paragr. Heading 1 Mary B., a 30-year-old woman with a history of intravenous (IV) drug use, was seen at a health clinic with complaints of swollen glands, fatigue, fever, weight loss, fre- quent vaginal yeast infections, and a persistent skin rash that had not responded to treatment. The physician ordered the laboratory tests shown in Tables 4...5 to 4.7. is Table 4-5 COMPLETE BLOOD COUNT Mary B. WBC RBC Hb Het MCV MCH...
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