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CAN SOMEONE VERIFY QUESTION 1 AND EXPLAIN QUESTION 2. WHAT ARE THE CARBOXY AND AMINO (N)...

CAN SOMEONE VERIFY QUESTION 1 AND EXPLAIN QUESTION 2. WHAT ARE THE CARBOXY AND AMINO (N) ENDS AND HOW DO I RECOGNIZE THEM? WILL LEAVE RATE AND COMMENT!

The following is an mRNA transcribed from a prokaryotic gene.

5’-UCAUAAGGAUGCCACCAACCGGCGGAUCAGGCCCGUGACACCUUGG-3’

1. Write the corresponding double-stranded DNA sequence the mRNA is transcribed from. Indicate the 5’ and 3’ ends of both strands and indicate the coding and template strands.   

3’-AGTATTCCTACGGTGGTTGGCCGCCTAGTCCGGGCACTGTGGAACC-5’

2. Write the amino acid sequence of the encoded polypeptide. Indicate the amino (N) and carboxy (C) ends of the polypeptide.

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Answer #1

Q1. Ans :

This is the DNA double stranded sequence, from the mRNA given in question transcribed from : -

5'-TCATAAGGATGCCACCAACCGGCGGATCAGGCCCGTGACACCTTGG-3' - Coding Strand

3'-AGTATTCCTACGGTGGTTGGCCGCCTAGTCCGGGCACTGTGGAACC-5’ - Templete Strand

Q2. Ans:

5’-UCAUAAGGAUGCCACCAACCGGCGGAUCAGGCCCGUGACACCUUGG-3’
The Amino Acid sequence of the encoded polypeptide are followings :-

N-terminal (Amino) end Met-Pro-Pro-Thr-Gly-Gly-Ser-Gly-Pro C-terminal (Carboxy) end.

AUG : Start Codon

UGA : Stop Codon

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