Question

1.) In which direction is RNA transcribed?   

2.) Which of the two strands (A or B) serves as the TEMPLATE strand for the transcription of a mRNA that contains both a start and a stop codon?   

3.) Which number (1, 2, 3, 4, or 5) best approximates the location of the -10 consensus sequence?   

4.) How many amino acids long is the protein encoded by the mRNA from this DNA sequence?   

5.) What is the second amino acid encoded by the mRNA from this DNA sequence?   

Use the following genomic DNA sequence to answer the questions. 5 A 1 2 3 5- -CTTGGTATTCAATAGCTTAAGTTCATACGTGC- 3--------GAACCATAAGTTATCGAATTCAAGTATGCACG- -3 --5 B 1.) In which direction is RNA transcribed? 5' --> 3 2.) Which of the two strands (A or B) serves as the TEMPLATE strand for the transcription of a mRNA that contains both a start and a stop codon? B 3.) Which number (1,2,3,4, or 5) best approximates the location of the - 10 consensus sequence? 3 4.) How many amino acids long is the protein encoded by the mRNA from this DNA sequence? 3 5.) What is the second amino acid encoded by the mRNA from this DNA sequence? Leucine 6.) What is the tRNA anticodon sequence that binds to the third codon of the transcribed mRNA? VAC Question 4 2 pts What are the two remaining stop codons that were NOT used in the previous question? Pro

6.) What is the tRNA anticodon sequence that binds to the third codon of the transcribed mRNA?

Answer 1

1. All nucleic acids are synthesized in 5'-3' direction only.
RNA is also transcribed in 5'-3' direction.

2. The top strand (A) serves as the template strand for the transcription.
The template strand is complementary to the mRNA

3. The -10 consensus sequence is located upstream to the transcription start site = 5

4. The template DNA sequence: 5'-ATGAACTTAAGCTATTGA-3'
The mRNA sequence: 5'-AUGAACUUAAGCUAUUGA-3'
The amino acid sequence: Met-Asn-Leu-Ser-Tyr
Number of amino acids = 5

5. The second amino acid = Aspargine

6. Third codon = 5'-UUA-3'
Anticodon = 5'-UAA-3'

Q4. Termination codon in the given sequence = UGA
Remaining termination codons = UAA and UAG