Homework Answers
All of the above except 4.
In real time PCR and PCR , primers get hybridizes to DNA. In DNA sequencing a short stretch of oligonucleotide is also hybridized to DNA. In southern Blotting complementary DNA is hybridizes to the Target DNA and in Northern Blotting RNA probe is hybridizes to complementary RNA .
In restriction digestion DNA is cutted with restriction enzymes only.
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- Which technique requires "hybridization” ? 1. PCR 2. Southern or Northern blotting 3. DNA sequencing...
The general term Choose... A hybridization B Southern blotting C PCR D annealing refers to the...
The general term Choose... A hybridization B Southern blotting C PCR D annealing refers to the process in which single-stranded nucleic acids (DNA or RNA) are allowed to interact so that complexes are formed between molecules with complementary sequences.
Which of the following statements is FALSE about Southern blotting? a) smaller nucleic acid fragments move...
Which of the following statements is FALSE about Southern blotting? a) smaller nucleic acid fragments move faster than larger nucleic acid fragments in gels b) a hybridization probe for Southern blot can be single stranded DNA or RNA c) In most cases, DNA is cut with a restriction enzyme prior to gel electrophoresis d) smaller hybridization probes will always detect smaller DNA fragments than larger probes e) Southern blotting is used to analyze DNA
6) Many scientists doubted that shotgun sequencing would work, even with the smallest genomes, because: a....
6) Many scientists doubted that shotgun sequencing would work, even with the smallest genomes, because: a. There would not be overlaps between the different mini-sequences. b. Computers would be unable to handle the huge amount of data generated by a shotgun sequencing project. c. Small prokaryotic genomes contain large amounts of repetitive DNA. d. No method existed for breaking genomic DNA into random fragments. 7) In a PCR-RFLP assay, enzyme digestion revealed three bands (152+187+462bp) in AA animals, four bands...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in...
14. Restriction endonucleases are a. enzymes that restrict DNA synthesis b. enzymes that cut DNA in specific sequences c. nuclear proteins that are involved in transcription d. components of the ribosomes involved in protein synthesis 15. The first step in southern blotting is a. converting DNA into RNA b. cutting high molecular weight DNA into smaller pieces c. converting RNA into DNA d. radioactively labeling the DNA so it can be detected after the procedure is complete 16. The major...
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2....
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2. Which vehicles are often used for gene therapy to carry a healthy gene? Bacteria Viruses Plastic capsules Both A and B Both A and C 3. A common method in biochemistry to detect and analyze proteins is: Northern blot Southern blot Western blot Eastern blot All of the above 4. The technique used to identify a specific DNA sequence in bacterial colonies is In...
Question 2 Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence...
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
The map below illustrates three alleles in a genome segment. The alleles differ in the number...
The map below illustrates three alleles in a genome segment. The alleles differ in the number and location of restriction sequences. Restriction digestion and Southern blotting with the molecular probe whose hybridization location is indicated results in detection of a single DNA band for each allele. a. List the size of the DNA bands that would be detected for each of the following genotypes: D^2 D^2: D^2 D^3: D^3D^3: b. Restriction-digested DNA from two organisms is analyzed by Southern blotting....
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a)...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What...
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing...
Which of the following is true regarding DNA sequencing? (multiple answers possible) a. Next generation sequencing is limited to simultaneous sequencing of 23 different nucleotide chains at a time b. Next generation sequencing or “sequencing by synthesis”, requires ddNTPs c. DNA fragments generated by the Sanger sequencing reaction each contain a primer incorporated at the 5’ end of the nucleotide chain. d. Primers are labeled with different flurophores allowing all 4 chain termination reactions for Sanger sequencing to be combined...
6) Many scientists doubted that shotgun sequencing would work, even with the smallest genomes, because: a. There would not be overlaps between the different mini-sequences. b. Computers would be unable to handle the huge amount of data generated by a shotgun sequencing project. c. Small prokaryotic genomes contain large amounts of repetitive DNA. d. No method existed for breaking genomic DNA into random fragments. 7) In a PCR-RFLP assay, enzyme digestion revealed three bands (152+187+462bp) in AA animals, four bands...
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
The map below illustrates three alleles in a genome segment. The alleles differ in the number and location of restriction sequences. Restriction digestion and Southern blotting with the molecular probe whose hybridization location is indicated results in detection of a single DNA band for each allele. a. List the size of the DNA bands that would be detected for each of the following genotypes: D^2 D^2: D^2 D^3: D^3D^3: b. Restriction-digested DNA from two organisms is analyzed by Southern blotting....
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
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