Which of the following best describes how the migration of DNA in an agarose gel will...
Which of the following best describes how the migration of DNA in an agarose gel will be changed following incubation of the DNA with a topoisomerase?
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Gel migration is changed, because nucleotides are isomerized from the D to L configuration |
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Gel migration is changed, because compactness is changed |
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Gel migration is changed, because the total charge of the phosphate backbone is reduced |
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Gel migration is unaffected, because the number of base pairs is increased |
Homework Answers
The migration of DNA in agarose gel will change due to change in compactness of the DNA molecule, since the mobility is also affected by the compactness and conformation of the DNA molecule. The incubation with topoisomerase results in production of nicks, which changes the compactness of the molecule.
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Here is the only other info I have. Thanks!
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Please give clear answers along
with detailed explanations. Thank you so much!
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restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
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need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
Please correct all questions because i did them wrong
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