10) Define bacterial transformation? How did Frederick Griffith demonstrate that bacterial strains could be genetically transformed?
10) Define bacterial transformation? How did Frederick Griffith demonstrate that bacterial strains could be genetically transformed?
Homework Answers
bacterial transformation is the process by which bacteria take up the DNA from the environment.
Griffith used Streptococcus pneumonia for his studies, one strain R strain is not virulent and S strain is virulent.
S strain when injected into the Mouse, mouse dies, when R strain is injected moused remains alive.
Heat killed S strain when injected into a mouse, mouse remains alive.
when both heat-killed S strain and live R strain injected into mouse, the mouse dies, this shows the R strain is transformed by the S strain so that the R strain become virulent.
R strain has taken DNA from the S strain and become virulent.
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10) Define bacterial transformation? How did Frederick Griffith
demonstrate that bacterial strains could be genetically
transformed?
1. Who proposed the original central dogma of molecular biology? Draw the original central dogma that...
1. Who proposed the original central dogma of molecular biology? Draw the original central dogma that depicts the flow of information transfer, name each step and indicate the enzyme involved in each step. In the modified central dogma what two steps were added and which enzymes perform these steps? 2. How did Griffith demonstrate that bacterial strains could be genetically transformed?
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D 18) How did Avery, MacLeod, and McCarty demonstrate that Griffith's transforming principle was DNA? A)...
D 18) How did Avery, MacLeod, and McCarty demonstrate that Griffith's transforming principle was DNA? A) Injecting live S-strain bacteria alone could not kill a mouse. B) Injecting heat-killed S-strain bacterial alone could not kill a mouse. C) Heat-killed S-strain bacterial transformed live R-strain bacteria into live S-strain bacteria. D) DNase, an enzyme that destroys DNA, eliminated the biological activity of the transforming material. E) RNase, an enzyme that destroys RNA, had no effect on the transforming principle.
You have three bacterial strains: one is resistant to kanamycin (strain A), another is resistant to...
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Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
QUESTION 2 ow did Grifth perfrm his transformation experiments? O He mixed a heat-killed pathogenic strain...
QUESTION 2 ow did Grifth perfrm his transformation experiments? O He mixed a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain to convert some of the living cells into the pathogenic form. O He infected mice with a pathogenic strain of bacteria to test whether the mice can become resistant to the pathogen (i.e., they are transformed). O He mixed strains together to test which grew the fastest. O He mixed two pathogenic strains of bacteria to test...
1. Descri be the process of a bacterial transformation. Explain how scientists can make bacteria take...
1. Descri be the process of a bacterial transformation. Explain how scientists can make bacteria take up DNA in a laboratory setting. (15 pts.) 2. Genetic transformations are not limited to bacteria. There are real-world applications where scientists have taken genes from one organism and inserted them into another organism. Give an example of how a genetically modified organism (can be any organ- ism, you are not limited to bacteria) have been used to solve real-world issues. Describe the organisms...
9) What is the minimum number of genes needed to sustain life? How did researchers demonstrate...
9) What is the minimum number of genes needed to sustain life? How did researchers demonstrate that this minimum set of genes could sustain life?
According to the article How Microbes Defend and Define Us, bacterial populations on a person’s left...
According to the article How Microbes Defend and Define Us, bacterial populations on a person’s left hand differ significantly from those present on a person's right hand. Come up with a hypothesis for why this may be the case. Next, briefly describe a way that you could test your hypothesis through a controlled experiment.
Define latent heat of transformation. Using the data in the table. determine how many calories are...
Define latent heat of transformation. Using the data in the table. determine how many calories are needed to change of solid 10 degree C to at 210 degree C.
1. Who proposed the original central dogma of molecular biology? Draw the original central dogma that depicts the flow of information transfer, name each step and indicate the enzyme involved in each step. In the modified central dogma what two steps were added and which enzymes perform these steps? 2. How did Griffith demonstrate that bacterial strains could be genetically transformed?
D 18) How did Avery, MacLeod, and McCarty demonstrate that Griffith's transforming principle was DNA? A) Injecting live S-strain bacteria alone could not kill a mouse. B) Injecting heat-killed S-strain bacterial alone could not kill a mouse. C) Heat-killed S-strain bacterial transformed live R-strain bacteria into live S-strain bacteria. D) DNase, an enzyme that destroys DNA, eliminated the biological activity of the transforming material. E) RNase, an enzyme that destroys RNA, had no effect on the transforming principle.
QUESTION 2 ow did Grifth perfrm his transformation experiments? O He mixed a heat-killed pathogenic strain of bacteria with a living nonpathogenic strain to convert some of the living cells into the pathogenic form. O He infected mice with a pathogenic strain of bacteria to test whether the mice can become resistant to the pathogen (i.e., they are transformed). O He mixed strains together to test which grew the fastest. O He mixed two pathogenic strains of bacteria to test...
1. Descri be the process of a bacterial transformation. Explain how scientists can make bacteria take up DNA in a laboratory setting. (15 pts.) 2. Genetic transformations are not limited to bacteria. There are real-world applications where scientists have taken genes from one organism and inserted them into another organism. Give an example of how a genetically modified organism (can be any organ- ism, you are not limited to bacteria) have been used to solve real-world issues. Describe the organisms...
Define latent heat of transformation. Using the data in the table. determine how many calories are needed to change of solid 10 degree C to at 210 degree C.
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