Question

The following is the DNA sequence of the wild type allele of genr Z that you want to amplify using the polymerase chain reaction (PCR). Circle the set(s) of primers from the options below, which you would use for PCR reaction. Circle all that apply.

10. The following is the DNA sequence of the wild type allele of Gene Z that you want to amplify using the polymerase chain reaction (PCR). 5' CTCGAGGTGAATATGAAAG---- 3'GAGCTCCACTTATACTTTC---- Gene Z ---CATTTGGCGCGTAATCGATA3 ---GTAAACCGCGCATTAGCTATS! Circle the set(s) of primers from the options below, which you would use for PCR reaction. Circle all that apply. Set 1: 5'TACACTTATACTTTC3' and 3'GTAAACCGCGCATTAGS' Set 2: 5'CTCGAGGTGAATAT3' and 3'CCGCGCATTAGCTATS Set 3: 5'GAGTTACACTTATAC3 and 3'TGGCGAGTAATCGATAS

Answer 1

Set 2

There are two primers, forward and reverse.

Forwars primer binds to 3' end of lower strand.

Reverse primer binds to 3' end of upper strand.

A primer always binds to 3' end.

A primer is always complementary to DNA region where it binds.

Forward primer -

It will bind at 3' end of lower strand and will be complementary to it. It is also similar to 5' end of upper strand.

Reverse primer -

It will bind to 3' end of upper strand and will be complementary to it.

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