Question

Below is a sequence of double-stranded DNA that will be used as a template in the polymerase chain reaction (PCR). The goal is to use this as a template to make a a PCR product that includes the shaded area. The sequence of one of the PCR primers is given below.

Template DNA:

5' - CTTAGCGCTGTTGGGGGCCAACTATCACACACACCACACACAGGTATAAATGGCATTTGATACAGATTG - 3'

3' - GAATCTGTATCAAATGCCATTTATACCTGTGTGTGGTGTGTGTGATAGTTGGCCCCCAACAGCGCTAAG - 5'

If the sequence of one of the primers is 5' TTGTGGGGCC 3' which of the following primers would work as the other primer in PCR to produce a product that included the shaded region?

Select one:

a. 5’-ACACACACAC-3’

b. 5’-TTTTGATACAG-3’

c. 5’-TCAAAATGCC-3’

(will comment and upvote if correct answer)

Answer 1

Answer:

Primer which would work as the other primer in PCR to produce a product that included the shaded region are:

5'-TCAAAATGCC-3'

Because it is only primer that contains both start and stop codon sequence.

Hence,option(C) is correct.