Question

Q1 The immediate template for PCR amplification is double stranded DNA. (True or False)

Q2 A primer is a DNA oligonucleotide. (True or False)

Q1 Denaturation of DNA double helix takes place at 96 C. (True or False)

Q2 A primer is a single stranded DNA oligonucleotide. (True or False)

Q1 16 doubled stranded DNA fragments are obtained from one DNA double stranded template after 4 cycles of PCR. (True or False)

Q2 Denaturation of DNA double helix takes place at 72 C. (True or False)

Answer 1

I believe the correct answer to be:;

Q1 The immediate template for PCR amplification is double stranded DNA. (True or False)

FALSE, It is single stranded DNA molecule.

Q2 A primer is a DNA oligonucleotide. (True or False)

TRUE

Q1 Denaturation of DNA double helix takes place at 96 C. (True or False)

TRUE

Q2 A primer is a single stranded DNA oligonucleotide. (True or False)

TRUE

Q1 16 doubled stranded DNA fragments are obtained from one DNA double stranded template after 4 cycles of PCR. (True or False)

TRUE, As the number of DNA molecule doubles after each cycle.

Q2 Denaturation of DNA double helix takes place at 72 C. (True or False)

FALSE, as it occurs at 95 degree celcius.

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