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ich of the following solutions using...
Petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml...
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
Question 6 Using stock solutions to make up a solution: from final concentration to volume You...
Question 6 Using stock solutions to make up a solution: from final concentration to volume You need to digest a sample of DNA with a restriction enzyme. You will do this reaction later in the semester. The buffer for this reaction has a final concentration of 40 mM Tris, pH 7.5, 10 mM MgCl2 and 50 mM KCl. You have the following stock solutions: 0.5 M Tris, pH 7.5, 1 M MgCl2 and 2 M KCl. How would you make...
please help solve this and show all work clearly, thanks 6. The recipe for cell culture media is: 10 % serum, 1x ant...
please help solve this and show all work clearly, thanks
6. The recipe for cell culture media is: 10 % serum, 1x antibiotics and 2 mM Glutamine in basal media. You are given the following stock solutions: • Basal media • Serum 100% • Antibiotics 100x solution Glutamine 200 mm How will you prepare the 500 mls of media? (2 point) (Note: A 100X solution is 100 times more concentrated than the concentration required in the final solution. For example...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
i need all POST-LAB QUESTIONS (Type in your answers to the questions below) 1. In Activity...
i need all
POST-LAB QUESTIONS (Type in your answers to the questions below) 1. In Activity 1 how would the amount of NaCl produced be affected (more, less, remain the same) if instead of adding 2 mL of 1 M NA,CO, you added 4 ml. Explain. 2. In Activity 6 how would the amount of NaCl produced be affected (more, less, remain the same) if instead of adding 2 mL of 1 M HCl you added 4 ml. Explain. 3....
need help on these Suppose you obtained the following data from two standard solutions of known...
need help on these
Suppose you obtained the following data from two standard solutions of known concentrations using a UV-Vis spectroscope: Analysis # Conc. (mg/L) 8.00 20.00 Absorbance (at 255 nm) 0.160 0.390 What is the concentration (in mg/L) of an unknown solution with an absorbance of 0.200 at 255 nm 10 points)? The unknown solution contains the same analyte as the standard solution does (Assume a path length of 1cm). 10. You prepared a stock solution by dissolving a...
I need help with the following questions 1,2,3 the molarity for Sodium Carbonate is .50 and for Calcium Nitrite is .25 t...
I need help with the following questions 1,2,3 the molarity for
Sodium Carbonate is .50 and for Calcium Nitrite is .25 the volume
for both is 100mL
Lab 7 Post-Lab Upload Write out the molecular, ionic, and net ionic equations for the precipitation reaction (you should already have these in your notebooks from lab. Make sure the molecular equation is properly balanced, we will use it for the below calculations. Molecular: Naz CO3(aq) + Ca(NO3)2(aq) - 2 NaNO3)(aq) + CaCO3...
(3) (i) How many mLs of a 0.5 M KH2PO4 stock solution are needed to make...
(3) (i) How many mLs of a 0.5 M KH2PO4 stock solution are needed to make 250 mLs of a 2.5 mM K solution (1 mark)? (ii) How many g of Ca(NO3)2 are needed to make 300 mLs of a 1 M Ca(NO3)2 stock solution (1 mark)? (iii) What is the concentration of Nations in the -Mg nutrient treatment in mM and ppm as given in Table 1 (1 mark)? (iv) What is the concentration of Cl- ions in the...
Consider the following TPN medication order (Table 1) and the Stock Solutions (Table 2); then determine...
Consider the following TPN medication order (Table 1) and the Stock Solutions (Table 2); then determine the exact volume of each component that should be used to compound the preparation. Round to the nearest whole mL. To receive all credit, show all work. TABLE 1 Adult Total Parenteral Nutrition Central Line Total TPN Volume : _____________ (please supply) (1pt) Qs with H20:________________ (please supply) (1pt) To make 2400mL + 100mL overfill. Rate: _________________mL/hr (please supply rate for a 24 hour...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
9,10,12
petermine the amounts/volumes of the chemicals needed to prepare the following solutions s 100 ml of O.1 N HC from concentrated (12.0 M) HO tyou will be asked to make this solution as practicel & 100 ml of L.0 M Tris pt-76 MW of Tris base is 121 g/mole 7. pH 7.6 is close to a neutral pH. When making 1.0 M Tris base, would you expect to or NaOH to adjust the pH to 7.67 to need HC...
please help solve this and show all work clearly, thanks
6. The recipe for cell culture media is: 10 % serum, 1x antibiotics and 2 mM Glutamine in basal media. You are given the following stock solutions: • Basal media • Serum 100% • Antibiotics 100x solution Glutamine 200 mm How will you prepare the 500 mls of media? (2 point) (Note: A 100X solution is 100 times more concentrated than the concentration required in the final solution. For example...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
i need all
POST-LAB QUESTIONS (Type in your answers to the questions below) 1. In Activity 1 how would the amount of NaCl produced be affected (more, less, remain the same) if instead of adding 2 mL of 1 M NA,CO, you added 4 ml. Explain. 2. In Activity 6 how would the amount of NaCl produced be affected (more, less, remain the same) if instead of adding 2 mL of 1 M HCl you added 4 ml. Explain. 3....
need help on these
Suppose you obtained the following data from two standard solutions of known concentrations using a UV-Vis spectroscope: Analysis # Conc. (mg/L) 8.00 20.00 Absorbance (at 255 nm) 0.160 0.390 What is the concentration (in mg/L) of an unknown solution with an absorbance of 0.200 at 255 nm 10 points)? The unknown solution contains the same analyte as the standard solution does (Assume a path length of 1cm). 10. You prepared a stock solution by dissolving a...
I need help with the following questions 1,2,3 the molarity for
Sodium Carbonate is .50 and for Calcium Nitrite is .25 the volume
for both is 100mL
Lab 7 Post-Lab Upload Write out the molecular, ionic, and net ionic equations for the precipitation reaction (you should already have these in your notebooks from lab. Make sure the molecular equation is properly balanced, we will use it for the below calculations. Molecular: Naz CO3(aq) + Ca(NO3)2(aq) - 2 NaNO3)(aq) + CaCO3...
(3) (i) How many mLs of a 0.5 M KH2PO4 stock solution are needed to make 250 mLs of a 2.5 mM K solution (1 mark)? (ii) How many g of Ca(NO3)2 are needed to make 300 mLs of a 1 M Ca(NO3)2 stock solution (1 mark)? (iii) What is the concentration of Nations in the -Mg nutrient treatment in mM and ppm as given in Table 1 (1 mark)? (iv) What is the concentration of Cl- ions in the...
Search .l LTE 2:48 AM C 77% x Bio 212 - Lab 5 - Solutions Il Repeat Proble... ... Lab 5: Additional Dilution Problems You have a stock solution that contains 24 mg/ml of protein, You want to make a set of standards with the following concentrations: 12 mg/ml, 6 mg/ml, 3 mg/ml and 1.5 mg/ml. You need a minimum of 500 ul of each standard after all dilutions are completed. Using the technique of serial dilution, explain how you...
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