We did an assay for apyrase activity. What was the function of acid molybdate in that...
We did an assay for apyrase activity. What was the function of acid molybdate in that assay?
Homework Answers
Phosphate test is used to determine the concentration of phosphate present in a solution. In the given assay, Apyrase releases Pi from substrate (AMP). The released Pi is quantified using ammonium molybdate.
Apyrase
AMP ---------------> Adenine + Pi
(NH4)2MoO4 + Pi --------> H3PMo12O40
H3PMo12O40 + HMG+2 --------> MG+ (H2PMo12O40) + 2H+
HMG+2: Malachite green: Yellow in colour, Lambda max = 446 nm
MG+ (H2PMo12O40): Green in colour, Lambda max = 640 nm
Spectrometer readings for the absorbance values can be plotted against a standard curve to calculate the activity of the enzyme.
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