Homework Answers
Add Answer to:
2. Draw where you would predict to see the bands on a protein gel for the...
A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein,...
A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but...
Help please 2) The following image shows an SDS-PAGE gel: 1 2 3 4 - 5...
Help please
2) The following image shows an SDS-PAGE gel: 1 2 3 4 - 5 6 7 974 kDa 66.2 kDa 45.0 kDa 31.0 kDa 21.5 kDa 14.4 kDa Please mark the only statement that could be accurately made using this SDS-PAGE gel. A. The protein in highest concentration in lane 4 is basic. B. The protein in highest concentration in lanes 5, 6 and 7 has a molecular weight of less than 66.2 kilodaltons. C. The protein with...
Dear anon Yes it is, does that mean I can't ask questions?? Problem 4.1.6 (tags: #gel...
Dear anon Yes it is, does that mean I can't ask
questions??
Problem 4.1.6 (tags: #gel electrophoresis, #drug treatments, #protein glycosylation) In vitro protein synthesizing systems, containing free amino acids, mRNA, and cytosolic extracts, can be used to study protein synthesis. The cytosolic extracts provide ribosomes and all cellular proteins and factors necessary for protein synthesis. The gel on the right is a protein gel showing the results of experiments conducted using this in vitro system, the mRNA of a...
For reference- Example Ponceau S Stained nitrocellulose membrane (NCF) Samples in each lane 1: SeeBlue 2...
For reference-
Example Ponceau S Stained nitrocellulose membrane (NCF)
Samples in each lane
1: SeeBlue 2 Prestrained Protein Markers
2: Purified IgY from egg yolk
3: Bacterial cell extract (E. coli cell extract)
4: Mammalian cell extract (Ox heart cell extract)
How many proteins bands should have been visible the Igy sample lane after Ponceau S staining? (1 mark) Answer: What sizes did you see, or would expect to see for the Igy sample? Note: based on your answer to...
SDS Page Gel: The provided standard protein sample for electrophoresis consists of 9 polypeptides with molecular...
SDS Page Gel:
The provided standard protein sample for electrophoresis
consists of 9 polypeptides with molecular weights ranging from 250
to 15 KDa.
Sample 1: Protein A in a sample buffer with
B-Mercaptoethanol
Sample 2: Protein A in a sample buffer without
B-Mercaptoethanol
Sample 3: Protein B in a sample buffer with
B-Mercaptoethanol
Sample 4: Protein C in a sample buffer without
B-Mercaptoethanol
Use the picture below & the information about the proteins
above to answer the following questions.
1a....
Confused on how to answer questions E, F, and G. The answer for E is Cysteine,...
Confused on how to answer
questions E, F, and G.
The answer for E is Cysteine,
F (i) is 2
F (ii) is 2
F (iii) is 180
Question 5. Protein X, a protein that binds tyrosine tightly, can be purified in three chromatography steps: 1) affinity chromatography using a tyrosine resin, 2) gel filtration chromatography using a resin with a separation range of 30-200 kDa, and 3) anion exchange chromatography run at pH 7.6. The elution profiles for Steps...
Work out 9C and 9D using info given please. No additional info is needed. Workout all parts of number 9. Explain in...
Work out 9C and 9D using info given please. No additional info
is needed.
Workout all parts of number 9. Explain in clear details on how to draw a gel, why and how to determine where the bands will be formed. 9. The following proteins were identified in a mixture. protein MW (kDa) 38 4.8 [MgSO.) resured to precious 45% 80% 65% 20% 30% 45% 6.8 9.50 5.3 115 a) What is meant by "salting out a protein using MgSO:?...
Carl has just finished purifying a protein and analysis by gel filtration indicated that the molecular...
Carl has just finished purifying a protein and analysis by gel filtration indicated that the molecular weight of the native (undenatured) protein was 130,000 dalton. His advisor wanted him to determine whether this protein had quaternary structure using gel electrophoresis. He wants to be able to describe the molecular weight of each of the subunits and the forces involved in linking these subunits together (disulfide linkages and/or electrostatic, hydrogen bonding and hydrophobic interactions). Carl first analyzed his pure protein sample...
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or...
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or a variant of X that contains the amino acids KDEL at its C terminus, both of them under control of an inducible promoter, KDEL motifs are normally found on proteins located in the lumen of the ER. At 30 minutes after induction, cycloheximide, an inhibitor of protein synthesis, is added to the culture medium. At 30, 45, and 60 minutes after induction (that is,...
9. The following proteins were identified in a mixture. protein te precipitate MW (kDa) 4.8 22...
9. The following proteins were identified in a mixture. protein te precipitate MW (kDa) 4.8 22 IMRSO) 45% 80% 65% 20% 30% 45% 5.3 6.8 9.50 un 115 5.3 th(cont.) a) What is meant by "salting out" a protein using MgSO.? What happens on a molecular level? b) Draw a gel indicating proteins 1-6 order of elution in SDS-PAGE. Label where sample would start so I am clear on the validity of your answer. c) Draw their order of elution...
A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but...
Help please
2) The following image shows an SDS-PAGE gel: 1 2 3 4 - 5 6 7 974 kDa 66.2 kDa 45.0 kDa 31.0 kDa 21.5 kDa 14.4 kDa Please mark the only statement that could be accurately made using this SDS-PAGE gel. A. The protein in highest concentration in lane 4 is basic. B. The protein in highest concentration in lanes 5, 6 and 7 has a molecular weight of less than 66.2 kilodaltons. C. The protein with...
Dear anon Yes it is, does that mean I can't ask
questions??
Problem 4.1.6 (tags: #gel electrophoresis, #drug treatments, #protein glycosylation) In vitro protein synthesizing systems, containing free amino acids, mRNA, and cytosolic extracts, can be used to study protein synthesis. The cytosolic extracts provide ribosomes and all cellular proteins and factors necessary for protein synthesis. The gel on the right is a protein gel showing the results of experiments conducted using this in vitro system, the mRNA of a...
For reference-
Example Ponceau S Stained nitrocellulose membrane (NCF)
Samples in each lane
1: SeeBlue 2 Prestrained Protein Markers
2: Purified IgY from egg yolk
3: Bacterial cell extract (E. coli cell extract)
4: Mammalian cell extract (Ox heart cell extract)
How many proteins bands should have been visible the Igy sample lane after Ponceau S staining? (1 mark) Answer: What sizes did you see, or would expect to see for the Igy sample? Note: based on your answer to...
SDS Page Gel:
The provided standard protein sample for electrophoresis
consists of 9 polypeptides with molecular weights ranging from 250
to 15 KDa.
Sample 1: Protein A in a sample buffer with
B-Mercaptoethanol
Sample 2: Protein A in a sample buffer without
B-Mercaptoethanol
Sample 3: Protein B in a sample buffer with
B-Mercaptoethanol
Sample 4: Protein C in a sample buffer without
B-Mercaptoethanol
Use the picture below & the information about the proteins
above to answer the following questions.
1a....
Confused on how to answer
questions E, F, and G.
The answer for E is Cysteine,
F (i) is 2
F (ii) is 2
F (iii) is 180
Question 5. Protein X, a protein that binds tyrosine tightly, can be purified in three chromatography steps: 1) affinity chromatography using a tyrosine resin, 2) gel filtration chromatography using a resin with a separation range of 30-200 kDa, and 3) anion exchange chromatography run at pH 7.6. The elution profiles for Steps...
Work out 9C and 9D using info given please. No additional info
is needed.
Workout all parts of number 9. Explain in clear details on how to draw a gel, why and how to determine where the bands will be formed. 9. The following proteins were identified in a mixture. protein MW (kDa) 38 4.8 [MgSO.) resured to precious 45% 80% 65% 20% 30% 45% 6.8 9.50 5.3 115 a) What is meant by "salting out a protein using MgSO:?...
4) A cell line is transfected with a plasmid encoding either a secreted glycoprotein, X, or a variant of X that contains the amino acids KDEL at its C terminus, both of them under control of an inducible promoter, KDEL motifs are normally found on proteins located in the lumen of the ER. At 30 minutes after induction, cycloheximide, an inhibitor of protein synthesis, is added to the culture medium. At 30, 45, and 60 minutes after induction (that is,...
9. The following proteins were identified in a mixture. protein te precipitate MW (kDa) 4.8 22 IMRSO) 45% 80% 65% 20% 30% 45% 5.3 6.8 9.50 un 115 5.3 th(cont.) a) What is meant by "salting out" a protein using MgSO.? What happens on a molecular level? b) Draw a gel indicating proteins 1-6 order of elution in SDS-PAGE. Label where sample would start so I am clear on the validity of your answer. c) Draw their order of elution...
Most questions answered within 3 hours.
-
Can someone please help me add appropriate descriptive
comments to each line of code in the...
asked 2 minutes ago -
Romeo wishes to throw a bouquet of flowers to Juliet, who is on
a second-story balcony,...
asked 1 hour ago -
Why is QE a controversial monetary policy tool.
A. It may lead to excessive inflation.B. By...
asked 1 hour ago -
Principles of Programming midterm study guide help!
1.)
______ Which of the following would reference the...
asked 46 minutes ago -
A finite potential well has depth U0 = 2.78 eV . What is the
penetration distance...
asked 1 hour ago -
1. The bus bars of a power station are in two sections A and B
separated...
asked 1 hour ago -
Fiscal policy is the deliberate manipulation of taxes and
government spending to alter GDP, employment, inflation...
asked 2 hours ago -
evaluating an expression using only one digit and + and - as
operators ....3+5-1+7-5+8
-----------------------
stack...
asked 2 hours ago -
Two concentric current loops lie in the same plane. The smaller
loop has a radius of...
asked 3 hours ago -
1)Which of the following is an
important difference between qualified and nonqualified retirement
plans?
a. Qualified...
asked 3 hours ago -
What's the streaming business's problem on the
horizon?
asked 4 hours ago -
I need help with writing the conclusion for this online lab
report
Abstract
By testing the...
asked 4 hours ago