The correct option is 1st option i.e., 5'-TTTTTTTTTTTTTTTTTTTTT-3'
Explanation -
RNA is a single stranded polynucleotide chain. The formation of cDNA ( complementary DNA ) from the help of mRNA as a template strand is known as Reverse Transcription. The mRNA consists of poly(A) tail at the 3' end and this advantage is being utilised in Reverse Transcription. During the initial step of Reverse Transcription oligo (dT) primer is added
(5'-TTTTTTTTTTTTTTTTTTTTT-3'). This primer binds to the poly (A) tail and the further complementary base pairing starts.
please explain briefly When making cDNA from mRNA, what is the appropriate primer to use? Choose...
Explain the steps involved in making cDNA from mRNA, and how you would create a cDNA library. What type of vector would you use, and what steps would you need to take to prepare the cDNA for ligation with the vector?
Please explain the following question and answer all
29. The central dogma of molecular genetics is that DNA encodes an mRNA, and mRNA allows proteins to be made. In the lecture on making cDNA libraries there was a statement that jokingly) said "central dogma be damned". What is it about making a cDNA library that goes against the central dogma? A. although a primer is required to make a cDNA, the primer is simply a long run of "T's", B....
QUESTION 1 RNA poll initiates synthesis of the mRNA transcript without a primer True O False QUESTION 2 En The +1 position identifies the location for translation to begin when bound the mRNA is bound by the ribosomal subunit. True False QUESTION 3 The small ribosomal subunit binds the mRNA transcript at a sequence that is complementary to the gene promoter in order to initiate translation. O True False QUESTION 4 The 5' cap is necessary for protection from exonuclease...
1- Briefly describe how to choose an appropriate SmartArt layout. 2- Briefly discuss the importance of addressing your audience during a presentation that includes information graphics. 3- Discuss the importance of obtaining information for your graphics from credible sources and giving credit to those sources. 4-State five (5) general guidelines to consider when you have to include Information Graphics in a presentation. 5-List the steps involved in adding adding an Image to a Table.
This is an experimental design question. Please choose
one of the answers and briefly explain why.
In linear regression dignostics, how do we tell if the residuals follow a Normal Distribution or not? O By conducting an ANOVA analysis on the residuals. O By inspecting the normal probability plot to see if the points are randomly scattered. O By plotting the residuals and see if it is close to a bell curve. O By inspecting the normal probability plot to...
Question 3 2 pts Which should be part of your decision-making process when deciding which primer set to use? (none, one, or both are correct) No answer text provided Whether the primers were designed for member(s) of the same taxonomic group that you are studying Whether the locus is present in your target taxa
With the appropriate use of diagrams and clear descriptive text: •Explain briefly what is meant by tail-drop FIFO queuing. •Explain what is meant by BufferBloat, and what are the consequences for a VoIP flow's latency and throughput when a VoIP flow and bulk TCP flow share and congest a FIFO bottleneck queue. •Explain the role and operation of the active queue management (AQM) schemes known as PIE and FQ-CoDel. For both PIE and FQ-CoDel separately, explain how they impact on...
Can someone please explain this, I know its 5 -->3 then mRNA
is 3' -->5' but how and where does the tRNA come into play? I
thought the aminos/polypeptides where next?
A particular triplet of bases in the template strand of a coding sequence of DNA is 3-AAA-5. What is the anticodon on the tRNA that binds the corresponding mRNA codon? 5-UUU-3 O 5-TTT-3 5-UUA-3 5-AAA-3
dar Use the following DNA sequence to transcribe into the mRNA: 5'AAATGC3' (So what is the mRNA from this): 3.....5 12pt Paragraph O words </> P
Short Answer: Respond below using complete sentences where appropriate #9 Please briefly explain what a - AG tells you about the free energies associated with the reactants and 7pts the products of a reaction