Prokaryotes | Eukaryotes | |
Smaller subunits | 30S | 40S |
Larger subunit | 50S | 60S |
Energy source | GTP | GTP |
Shine dalgarno | present | - |
Kozak sequence | - | present |
Cap | - | present |
Tail | - | present |
Proteins that bind ribosome | required | required |
Start codon | AUG | AUG |
Initiator tRNA | formylayed methionine | methionine |
Elongation factors | required | required |
eEF 1 | - | present |
eEF 2 | - | present |
EF Tu | present | - |
EF G | present | - |
Termination codon | UAG, UGA, UAA | UAG, UGA, IAA |
eRF | - | present |
RF 1 | present | - |
RF 2 | present | - |
RF 3 | present | - |
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102. Fill in the chart comparing translation in prokaryotes vs. eukaryotes. Prokaryotes Eukaryotes Small ribosomal subunit...
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a stop codon. A release factor binds and causes the release of the new polypeptide, along with the mRNA. The ribosome dissociates. v Acharged tRNA with a matching anticodon binds the mRNA codon in the A site. ✓ The ribosome moves exactly 3 nucleotides toward the 3* end of the mRNA. The small ribosomal subunit uses rRNA to bind to the Kozak sequence, which places...
QUESTION 1 QUESTION 5 QUESTION 11 Identify the components required for translation initiation in bacteria What is the enzymatic component of the ribosome? A Protein Identify the TRANS components of the transcription initiation complex in bacteria ATFIE Bir RNA C. TATA BOX D-10 and 35 sequences E Signa factor B. Carbohydrates C.RNA CATFIE B. 5methyl guanosine cap C. Shine-Dalgamo Sequence D. Sigma factor CETFIID (TBP and TAFS) FTFIIB G. Initiator RNA H.10 and 35 sequences EL Smal ribosomal subunit J....
there are multiple answers correct MS Which of the following events occur during prokaryotic translation termination? The small ribosomal subunit binds with a specific tRNA to the mRNA and scans for a start codon. A tRNA recognizes the stop codon and the ribosome dissociates from the mRNA A protein recognizes the stop codon and the ribosome dissociates from the mRNA. A tRNA binds a codon and the ribosome adds amino acids from each tRNA to the polypeptide chain. Codons in...
Tetracycline inhibits protein synthesis by which mechanism? It interacts with the small ribosomal subunit, blocking access of the aminoacyl-tRNA to the mRNA-ribosome complex. It binds to the 305 subunit and distorts its structure, interfering with the initiation of protein synthesis. It binds irreversibly to a site on the 50S subunit of the bacterial ribosome, thus inhibiting translocation. It inactivates the eukaryotic elongation factor, eEF-2, thus preventing translocation.
* 1. Which of the following statements describes translation initiation in Eukaryotes: Initiation factors, a tRNAmet, and the ribosome's small subunit bind to the poly-A tail of mRNA. ✓ The ribosome's large subunit, tRNA, and P-site scan the mRNA for codons. ✓ The ribosome's large subunit binds to AUG codon on the mRNA. A ribosome binds to the C-terminus of an mRNA. Initiation factors, a tRNAmet, and the ribosome's small subunit bind to the 5'cap of mRNA. Initiation factors provide...
22. What are the roles of Dicer and RISC in the function of miRNAs? Dicer RISC 23. Describe the concepts of primary, secondary, tertiary and quaternary protein structure 24. Here is a short sequence of codons. AUG CAU UGU UUU Write out the amino acids this sequence of codons encodes. Now add an insertion mutation of your choosing in the first codon and write out the new mutant sequence. What are the first four amino acids encoded by this mutant...
choose the correct answer 26. In eukaryotes, but not in prokaryotes, ribosomes find the start site of translation by? a. binding directly to a ribosome-binding site preceding the initiation codon b. scanning along the mRNA from the 5' end c. recognizing an AUG codon as the start of translation. d. binding an initiator tRNA 27. Which of the following statements about prokaryotic mRNA molecules is FALSE? a. A single prokaryotic mRNA molecule can be translated into several proteins b. Ribosomes...
Match the following choices to the questions below. UUU catalyzes translocation of the ribosome involved in transcription associated with the binding of mRNA in the ribosome binds to Shine-Dalgarno sequence involved in replication contains information in the form of anticodons catalyzes the formation of amino acid-AMP AUG UAG binds to pribnow box catalyzes formation of peptide bonds catalyzes disassembly of the translation complex associated with the binding of tRNA in the ribosome catalyzes disassembly of the transcription complex start codon...
Which of the following statements correctly describes a DNA transposon? Flanking direct repeats are generated when a transposable element inserts into DNA. Transposable elements are mobile DNA sequences. A new copy of a transposable element is introduced at a new site, and the old copy always remains at the original site during replicative transposition. Transposable elements are thought to have played an important role in the evolution of many organisms. All of the above statements correctly describe a DNA transposon....
5) Translation was originally sorted out using a variety of inhibitors of protein synthesis Inhibitors tend to interrupt the process at a particular step and a population of translating ribosomes will all stop in the same state. An allied technique is to identify temperature sensitive mutations in various accessory factors. When the population of ribosomes is shifted to the restrictive temperature, ribosomes will stall when they need the function of the mutant protein to continue By analyzing the structure of...