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7. The following DNA sequence is from a virus that is dangerous and scientists want to...
RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To...
RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To begin the recombinant DNA process, scientists must first ide at codes for the production of the protein they want to manufacture. One is to go backwards from the amino acid sequence of the desired protein to ide sequence of the gene. After scientists have identified the gene, they m it. Restriction enzymes or endonucleases from bacterial cells are key in th ia produce restriction...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
The following is a small part of a vector DNA sequence showing the NdeI and SacII...
The following is a small part of a vector DNA sequence showing
the NdeI and SacII cut sites. The
bases recognizedby the enzymes are in bold. They both are Class II
restriction enzymes. These enzymes recognize a specific sequence of
palindromic bases, i.e., reads the same on both strands
1. When you cut this DNA, shown in the figure above, by
NdeI, it will generate sticky ends. Write the two pieces
of DNA that will result after NdeI digestion and...
i need from a to i please step by step Question 2 (50 points) The template...
i need from a to i please step by step
Question 2 (50 points) The template strand of a buman DNA and a plasmid are given below. The strands are read from left to right. Both DNAs are to be digested by the four base pair cutter restriction enzymes shown in the table Bacteria DNA: 3-ACAGACCATGGAGGCTCCCATGGTTAGCCTGAAGCTTCGA- 3'--CTTIGTGTOCCACCATGGCAGGTACCGAACCTCAACTTTCCTC.-5, Human DNA: Restriction Enzyme Name Ai Bi Ci Di Ei Sequence and type of eut GATC- 3 CTAG 5 T CGA-3 5- G...
Identify two restriction endonucleases that could be used to make sticky ends near the 5’ end of this DNA sequence (uppe...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
Write true or false ______ 1. The DNA sequence of one human being is on average...
Write true or false ______ 1. The DNA sequence of one human being is on average 99.9% identical to another random human being. ______ 2. As of 2009, all living human beings have had their entire genome sequenced. ______ 3. The nucleotide bases present in a DNA sequence are A, U, G, C. ______ 4. Techniques that enabled scientists to clone genes were developed in the 1970s. ______ 5. A restriction enzyme is useful because it is a generic enzyme...
Please solve Q4: Q1) This DNA sequence v is for an actual plasmid named pSB1A2-113521. It...
Please solve Q4:
Q1) This DNA sequence v is for an actual plasmid named pSB1A2-113521. It contains the 5' to 3' DNA sequence for constitutive production of a red fluorescent protein in bacteria, along with a ColE1 origin of replication and a resistance gene for the antibiotic ampicillin. The DNA is circular, so if one ran along the DNA and hit the end of the sequence, one would find themselves back at the beginning. The sequence of the complementary strand...
AIDS (acquired immunodeficiency syndrome), caused by the HIV virus is very common in Zaire. The virus...
AIDS (acquired immunodeficiency syndrome), caused by the HIV virus is very common in Zaire. The virus infects T cells of individuals by binding to a protein on the surface of T cells, named CCR5. Scientists observed that some people in Zaire never developed AIDS, even when they were exposed to the virus. They thought this could be due to the fact that CCR5 gene has two alleles in the Zairean population (CCR5-S (S); sensitive to AIDS; and CCR-R (R): resistant...
TRUE OR FALSE: 1. Base-pairing rules apply from one DNA strand to its partner, but not...
TRUE OR FALSE: 1. Base-pairing rules apply from one DNA strand to its partner, but not along the sugar-phosphate "handrails" of a DNA strand. 2. DNA polymerase cannot copy point mutations, so they are not passed on from parent cells to daughter cells in cell division. 3. It is not possible for a human gene to work in any other organism. 4. Mutations in regulatory DNA sequences may be more important to evolution than mutations in genes. 5. The enzyme...
Part B. Please CHOOSE FOUR of the following FIVE questions, indicating clearly which you want to...
Part B. Please CHOOSE FOUR of the following FIVE questions, indicating clearly which you want to be graded. Do not answer all of them!! (15 points each.) B1. Please consider the restriction enzymes Apal and PspOMI (OMG, who THINKS of these names???). Anyway, these enzymes have the following recognition/cut sequences, with the * indicating the cut site: Apali 5'G GGCC*C 3 3'C *C C G G G 51 PspOMI: 5' G *GG CCC 31 3' C C C G G#...
RECOMBINANT DNA: PLASMID VECTOR engineering is the direct manipulation of an organism's DNA using nology. To begin the recombinant DNA process, scientists must first ide at codes for the production of the protein they want to manufacture. One is to go backwards from the amino acid sequence of the desired protein to ide sequence of the gene. After scientists have identified the gene, they m it. Restriction enzymes or endonucleases from bacterial cells are key in th ia produce restriction...
Luestion 3 1 pts Review: You have the DNA that is radioactively labeled at the S'ends of DNA as shown below. But you want DNA that is labeled only at one end of the DNA, not both ends. One of the other undergraduate students in the lab suggests that you use a restriction enzyme to cut the DNA, then electrophorese the DNA in an agarose gel, then cut out the region of the gel with radioactive DNA fragment you want,...
The following is a small part of a vector DNA sequence showing
the NdeI and SacII cut sites. The
bases recognizedby the enzymes are in bold. They both are Class II
restriction enzymes. These enzymes recognize a specific sequence of
palindromic bases, i.e., reads the same on both strands
1. When you cut this DNA, shown in the figure above, by
NdeI, it will generate sticky ends. Write the two pieces
of DNA that will result after NdeI digestion and...
i need from a to i please step by step
Question 2 (50 points) The template strand of a buman DNA and a plasmid are given below. The strands are read from left to right. Both DNAs are to be digested by the four base pair cutter restriction enzymes shown in the table Bacteria DNA: 3-ACAGACCATGGAGGCTCCCATGGTTAGCCTGAAGCTTCGA- 3'--CTTIGTGTOCCACCATGGCAGGTACCGAACCTCAACTTTCCTC.-5, Human DNA: Restriction Enzyme Name Ai Bi Ci Di Ei Sequence and type of eut GATC- 3 CTAG 5 T CGA-3 5- G...
Identify two restriction
endonucleases that could be used to make sticky ends near the
5’ end of this DNA sequence (upper strand) so that
it could be incorporated into a new plasmid. You have a short list
of them in Table 9-2, and the specific, short sequences of bases
that other enzymes cut at are easily obtained from web resources.
You must cut as near to the 5' end as possible. Indicate the
specific sequences of bases for each endonuclease...
Please solve Q4:
Q1) This DNA sequence v is for an actual plasmid named pSB1A2-113521. It contains the 5' to 3' DNA sequence for constitutive production of a red fluorescent protein in bacteria, along with a ColE1 origin of replication and a resistance gene for the antibiotic ampicillin. The DNA is circular, so if one ran along the DNA and hit the end of the sequence, one would find themselves back at the beginning. The sequence of the complementary strand...
AIDS (acquired immunodeficiency syndrome), caused by the HIV virus is very common in Zaire. The virus infects T cells of individuals by binding to a protein on the surface of T cells, named CCR5. Scientists observed that some people in Zaire never developed AIDS, even when they were exposed to the virus. They thought this could be due to the fact that CCR5 gene has two alleles in the Zairean population (CCR5-S (S); sensitive to AIDS; and CCR-R (R): resistant...
Part B. Please CHOOSE FOUR of the following FIVE questions, indicating clearly which you want to be graded. Do not answer all of them!! (15 points each.) B1. Please consider the restriction enzymes Apal and PspOMI (OMG, who THINKS of these names???). Anyway, these enzymes have the following recognition/cut sequences, with the * indicating the cut site: Apali 5'G GGCC*C 3 3'C *C C G G G 51 PspOMI: 5' G *GG CCC 31 3' C C C G G#...
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