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Explain the logic behind the following problem and show the solution to the answer (which is...

Explain the logic behind the following problem and show the solution to the answer (which is choice C): An enzyme exhibiting Michaelis-Menton kinetics has a velocity of 0.2 Vmax at a substrate concentration of 1.0 mM. What substrate concentration is required to triple the velocity? A. 4mM B. 2.4mM C. 6mM D. 3mM E. 0.6mM

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Answer #1

Answer:

Vmax =0.2

[S]=1.0mM

0.2Vmax = Vmax[1 mM] / KM + [1mM];

solving for KM = 4 mM

0.6Vmax = Vmax [S] / 4 mM + [S];

The substrate concentration for triple the velocity is [S]= 6 mM

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