A ddNTP is often used in the Sanger sequencing process. Compared to the normal DNA precursors,...
A ddNTP is often used in the Sanger sequencing process. Compared to the normal DNA precursors, ddNTPs lack a(n)___ at the ___ carbon. Select one: OH, 3' H, 2' OH, 2' H, 3'
Homework Answers
ddNTP doesn't have 3'OH group,
So due to absence of the 3' OH group , it cannot detect by DNA polymerase, so when ddNTP come across to the amplification causes chain termination so it help for the sequencing of the DNA.
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A ddNTP is often used in the Sanger sequencing process. Compared
to the normal DNA precursors,...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing...
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing gel? A C GT a.5' - TTGGCCAAA - 3 6.5' - AAACCGGTT-3' OC. 5'- ATGGACTCA - 3 d. 5'-ACTCAGGTA - 3' e. 5' - TGAGTCCAT - 3'
Question 2 Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence...
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies...
24. Which three of the following six features are common to Illumina and Sanger sequencing technologies (you will get 1/3 for each correct answer and minus 1/3 for each incorrect answer): Select one or more: a. Both technologies use terminators b. Both methods require PCR amplification c. Both methods require a ligation step d. Both methods require a restriction endonuclease e. Both methods require primers f. Both methods were developed by scientists while under the influence of strong hallucinogenic drugs...
If you wish to sequence a long strand of DNA in one round of reactions, you...
If you wish to sequence a long strand of DNA in one round of reactions, you should: O A. Decrease the ddNTP/dNTP ratio O B. Increase the ddNTP/dNTP ratio O C. Use a shorter DNA primer O D. Add twice as much primer Based on this figure, the most likely error is: O A. The scientist forgot to add dNTPs to one of the reaction tubes. O O B. <label for="q7_4" id="lq7_4">The scientist did not denature the DNA strands</label> O...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are lacking a OH at the...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This...
Identification of unknown Bacteria by sequencing rDNA Having a little trouble understanding the PCR process. This is a lab we did and some homework questions regarding the sequencing rDNA to find unknown bacteria. I hope by answering these I can have better understanding of the process. The more descriptive the better. Thank you! 2. A. List the reagents used in preparing master mix for PCR and write one sentence about why each one was necessary. B. Now that you have...
The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
Next-generation sequencing involves: A. generating many short sequences from an intact, continuous DNA sequence. B. generating...
Next-generation sequencing involves: A. generating many short sequences from an intact, continuous DNA sequence. B. generating many short sequences from fragmented DNA. C. splicing together DNA fragments. D. adding multiple probes to fragmented DNA. What is the difference between a mutation and a polymorphism? A. A mutation can exist in a single person; a polymorphism must exist in at least 2% of the population. B. A mutation involves a single base pair; a polymorphism involves many base pairs. C. Mutations...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable for a few dozen nucleotides but then become increasingly difficult to road. It is determined that the chains are terminated to readily. What is the most likely cause? a. Too much of the dNTPs b. Insufficient fluorescent dye Too much fluorescent dye d. Incorrect primer e. Insufficient dNTPs Question of 20 Moving to another question will save this response Question 13 Which statement comparing...
Dideoxy NTPs (ddNTPs) are used in sequencing reaction to terminate DNA polymerization reaction at various chain lengths. Which OH group on the carbohydrate (ribose) molecule is deoxidized in ddNTP, thus causes DNA polymerization to stop? O OH group on 1' carbon is deoxidized to-H O OH group on 2' carbon is deoxidized to-H O OH group on 3' carbon is deoxidized to -H O OH group on 4' carbon is deoxidized to-H O OH group on 5' carbon is deoxidized...
What is the sequence of the template DNA used to make this Sanger (chain termination) sequencing gel? A C GT a.5' - TTGGCCAAA - 3 6.5' - AAACCGGTT-3' OC. 5'- ATGGACTCA - 3 d. 5'-ACTCAGGTA - 3' e. 5' - TGAGTCCAT - 3'
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
If you wish to sequence a long strand of DNA in one round of reactions, you should: O A. Decrease the ddNTP/dNTP ratio O B. Increase the ddNTP/dNTP ratio O C. Use a shorter DNA primer O D. Add twice as much primer Based on this figure, the most likely error is: O A. The scientist forgot to add dNTPs to one of the reaction tubes. O O B. <label for="q7_4" id="lq7_4">The scientist did not denature the DNA strands</label> O...
In DNA sequencing, ddNTPs differ from dNTPs in that ddNTPs are
lacking a OH at the
a.
2' carbon
b.
5' carbon
c.
3' carbon
please answer as many as you can!! I am low on questions and
could use the help!
Mother || Child II Male 1 Male 2 Results from a paternity test using DNA fingerprinting is shown. DNA was isolated from a mother, her child and 2 potential fathers. Primers designed to amplify different satellite DNA regions...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable for a few dozen nucleotides but then become increasingly difficult to road. It is determined that the chains are terminated to readily. What is the most likely cause? a. Too much of the dNTPs b. Insufficient fluorescent dye Too much fluorescent dye d. Incorrect primer e. Insufficient dNTPs Question of 20 Moving to another question will save this response Question 13 Which statement comparing...
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