How would we select for transformants in a Bacterial Transformation Lab. In your answer, be sure...
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How
would we select for transformants in a Bacterial Transformation
Lab. In your answer, be sure...
You are doing a bacterial transformation in Professor Carrico’s Genetics lab. As you are getting ready,...
You are doing a bacterial transformation in Professor Carrico’s Genetics lab. As you are getting ready, you realize that you are out of a critical solution, Calcium Chloride. You look at the bottle and find the Molecular Weight CaCl2 is listed as 110.98g/mol. You need 10 ml of a 2 molar solution. Briefly describe how you would proceed to make this solution.
Experiment 1 - Bacterial Transformation Questions 1. Pill out the following table with your predictions for...
Experiment 1 - Bacterial Transformation Questions 1. Pill out the following table with your predictions for the bacterial transformation Imake sure to keep a copy of your predictions on a separate paper for lab next Plate # | Plate Contents week). Experiment Growth +orPresent Color lf Growth Added Plasmid E coli + Plasmid E. coli - Plasmid E. coli + Plasmid E. coli 3a LB, Amp, X-gal .Plasmid E. coli 3b LB, Amp, X-gal Plasmid E. coli 1b LB 2a...
Please help me answer the following questions Lab 20. Selective and Differential Media culture, how would...
Please help me answer the following questions
Lab 20. Selective and Differential Media
culture, how would you determine which a mixed Gram negative rm anism was which? Be specific. how 4. If you were given Define selective vs. differential media. 5.
A. Your lab To see if you understand what you did in our lab, answer the...
A. Your lab To see if you understand what you did in our lab, answer the following based in the procedures for the restriction digest and the gel electrophoresis. 1. Using the PGLO map on p7 of the gel electrophoresis procedure, predict the size of the fragments generated by each enzyme EcoRI Hindill, and Pst! (the sizes you would expect to see on the gel.) (6 pts) Hindill -8 fragments were produced by the restriction enzyme. 2. Answer the calculation...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment...
Suppose that you carried out a Bacterial transformation of E. coli HA101 with pGLO plasmid experiment in the lab. During the experiment plates with bacteria were inoculated from +GLO and -GLO microfuge tubes (LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate). 14) Explain what kinds of bacterial growth you will find on each of 4 plates after incubation ((LB (-) plate, LB/amp (-) plate, LB/amp (+) plate, and LB/amp/ara (+) plate): under normal light and...
..Il Verizon LTE 10:36 PM 7 40% O X Virtual_Lab_Bacterial_Growth.d... ... Virtual Lab: The Bacterial Growth...
..Il Verizon LTE 10:36 PM 7 40% O X Virtual_Lab_Bacterial_Growth.d... ... Virtual Lab: The Bacterial Growth Curve BIOL221 - Spring 2020 Go to the following website to access the virtual lab: http://vlab, amrita edu/?sub=3&brch=73&sim=1105&cnt=1 You may need to create an account, go ahead and do so, its free, we will be using this site a lot over the next few weeks. Part 1: Theory and Background Make sure you are on the "Theory" Tab, read the entire page, then come...
Post lab: Show all calculation to receive credit for an answer. Be sure to indicate your...
Post lab: Show all calculation to receive credit for an answer. Be sure to indicate your final answer where appropriate 1 An amine complex of cobalt(III) is yellow-orange and has a larof 475 nm The standard curve below is for a set of standards measured at in a 1.8 cm cell. Use it to answer the questions that follow 08 0.7 05 0.5 Absorbance 04 03 02 09 0 0 0.05 0.1 0.25 0.9 035 15 0.2 Concentrata a Determine...
Select reagents from the table to show how you would carry out this transformation. If more...
Select reagents from the table to show how you would carry out
this transformation. If more than one route exists, use the one
that requires the fewest steps; in no case will more than 3 steps
be required.
Enter your selection as a series of letters for the reagents in the
order that you wish to use them, i.e. fca.
? CH2 COOH H₃C H3C a. Reagents HBr b. Mg, ether C. CO2, ether then H307 d. BH3, THF then...
Post lab: Show all calculation to receive credit for an answer. Be sure to indicate your...
Post lab: Show all calculation to receive credit for an answer. Be sure to indicate your final answer where appropriate. 1. An amine complex of cobalt(III) is yellow-orange and has a box of 475 nm. The standard curve below is for a set of standards measured at me in a 1.8 cm cell. Use it to answer the questions that follow. 0.8 0.7 0.6 0.5 Ab sorbance 0.4 0.3 0.2 0.1 0 0 0.05 0.1 02 0.25 0.3 0.35 0.15...
How would you carry out the synthesis below? Show the reagents needed for your transformation, but...
How would you carry out the synthesis below? Show the reagents
needed for your transformation, but do NOT draw the mechanism for
each step.
SHORT ANSWER #29: How would you carry out the synthesis below? Show the reagents needed for each step of your transformation, but do NOT draw the mechanisms for each step. (10 points) NH2 N
Experiment 1 - Bacterial Transformation Questions 1. Pill out the following table with your predictions for the bacterial transformation Imake sure to keep a copy of your predictions on a separate paper for lab next Plate # | Plate Contents week). Experiment Growth +orPresent Color lf Growth Added Plasmid E coli + Plasmid E. coli - Plasmid E. coli + Plasmid E. coli 3a LB, Amp, X-gal .Plasmid E. coli 3b LB, Amp, X-gal Plasmid E. coli 1b LB 2a...
Please help me answer the following questions
Lab 20. Selective and Differential Media
culture, how would you determine which a mixed Gram negative rm anism was which? Be specific. how 4. If you were given Define selective vs. differential media. 5.
A. Your lab To see if you understand what you did in our lab, answer the following based in the procedures for the restriction digest and the gel electrophoresis. 1. Using the PGLO map on p7 of the gel electrophoresis procedure, predict the size of the fragments generated by each enzyme EcoRI Hindill, and Pst! (the sizes you would expect to see on the gel.) (6 pts) Hindill -8 fragments were produced by the restriction enzyme. 2. Answer the calculation...
..Il Verizon LTE 10:36 PM 7 40% O X Virtual_Lab_Bacterial_Growth.d... ... Virtual Lab: The Bacterial Growth Curve BIOL221 - Spring 2020 Go to the following website to access the virtual lab: http://vlab, amrita edu/?sub=3&brch=73&sim=1105&cnt=1 You may need to create an account, go ahead and do so, its free, we will be using this site a lot over the next few weeks. Part 1: Theory and Background Make sure you are on the "Theory" Tab, read the entire page, then come...
Post lab: Show all calculation to receive credit for an answer. Be sure to indicate your final answer where appropriate 1 An amine complex of cobalt(III) is yellow-orange and has a larof 475 nm The standard curve below is for a set of standards measured at in a 1.8 cm cell. Use it to answer the questions that follow 08 0.7 05 0.5 Absorbance 04 03 02 09 0 0 0.05 0.1 0.25 0.9 035 15 0.2 Concentrata a Determine...
Select reagents from the table to show how you would carry out
this transformation. If more than one route exists, use the one
that requires the fewest steps; in no case will more than 3 steps
be required.
Enter your selection as a series of letters for the reagents in the
order that you wish to use them, i.e. fca.
? CH2 COOH H₃C H3C a. Reagents HBr b. Mg, ether C. CO2, ether then H307 d. BH3, THF then...
Post lab: Show all calculation to receive credit for an answer. Be sure to indicate your final answer where appropriate. 1. An amine complex of cobalt(III) is yellow-orange and has a box of 475 nm. The standard curve below is for a set of standards measured at me in a 1.8 cm cell. Use it to answer the questions that follow. 0.8 0.7 0.6 0.5 Ab sorbance 0.4 0.3 0.2 0.1 0 0 0.05 0.1 02 0.25 0.3 0.35 0.15...
How would you carry out the synthesis below? Show the reagents
needed for your transformation, but do NOT draw the mechanism for
each step.
SHORT ANSWER #29: How would you carry out the synthesis below? Show the reagents needed for each step of your transformation, but do NOT draw the mechanisms for each step. (10 points) NH2 N
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