Homework Answers
Add Answer to:
College students performed the following set of restriction digests on their plasmid pBLA 230. The following...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
A restriction map lists the locations of DNA sequences that are cut by a particular restriction...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme...
Suppose you are going to do a restriction digest with a plasmid, using the restriction enzyme Eco R1. A map of the plasmid is shown here. The entire plasmid is 6000 bp, and there are Eco R1 restriction sites at 1500 bp, 2000 bp, and 4000 bp. You’re going to run the entire volume of the digest on a gel, and you want to cut just enough DNA to have 50 ng in the smallest band on your gel. Starting...
question number 7 purifying plasmid from your cells, you will set up a teaction to cut...
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
You have determined that a bacterial strain you are working with contains a single type of...
You have determined that a bacterial strain you are working with
contains a single type of plasmid. You isolate the plasmid DNA and
digest separate portions of it with each of two different
restriction enzymes, BamHI and HpaI, and also perform a double
digest using both enzymes. You then fractionate the enzyme digests
on an agarose gel and stain the gel with ethidium bromideto
visualize the restriction fragment patterns. Your results are shown
below. Size markers (in nucleotide base pairs)...
Genetics Restriction Mapping A 7.0kb plasmid is cut at a Pstl site and a 4.0kb piece...
Genetics Restriction Mapping
A 7.0kb plasmid is cut at a Pstl site and a 4.0kb piece of DNA is inserted forming a recombinant plasmid. A restriction digest is conducted and then visualized with agarose gel electrophoresis. Construct a plasmid map of the recombinant fragment using the foliowing size information (in kb about the cuts produced by the digest. Map this plasmid. The way to present your answer is very specific, see example below. 7.0 Pstl 4.0 6.0 ECORI 5.0 8.9...
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel be...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
You have discovered a new plasmid in a species of foul-smelling, sewer-dwelling bacteria. As a first...
You have discovered a new plasmid in a species of foul-smelling, sewer-dwelling bacteria. As a first step towards understanding the composition of this plasmid, you digest the plasmid with a series of restriction enzymes, either alone or in combination. Using agarose gel electrophoresis, you determined the size of each fragment produced in these reactions and listed the result in the table below: A. How large is the plasmid in bp? B. Using the circle (plasmid) below, draw a restriction map...
Unanswered Question 6 0/1 pts A restriction digest is performed on a plasmid. The table below...
Unanswered Question 6 0/1 pts A restriction digest is performed on a plasmid. The table below shows the sizes of the resulting DNA fragments. 3 enzyme(s) present in the DNA size fragments digest resulting Pstl 10,000 base pairs (bp) Sspl 7,000 bp, 3,000 bp double digest, Estland 4,000 bp, 3,000 bp Sspl Explain the double digest results, what happened to create pieces of these sizes? Your Answer: 4/4 pts Question 7 List the 5 components needed to perform a PCR...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
A restriction map lists the
locations of DNA sequences that are cut by a particular restriction
enzyme for a piece of DNA, such as a chromosome or a plasmid.
Restriction maps are important when generating a construct for
experimental use. Digesting the DNA sequence with the restriction
enzymes will result in fragmented DNA of predictable sizes, based
on the restriction map, that allow a researcher to analyze if his
or her construct was generated correctly when visualized using gel
electrophoresis....
question number 7
purifying plasmid from your cells, you will set up a teaction to cut the plasmid DNA with the restriction enzyme Bgl Il. See the map below for the positions of the Bgl Il restriction enzyme sites on the plasmid. How many DNA fragments would you expect if your restriction enzyme digest was complete and your plasmid contains insert? Question 7 Not yet graded/ 1 pts After you electrophoresed the plasmid digest in Question 6, your gel showed...
You have determined that a bacterial strain you are working with
contains a single type of plasmid. You isolate the plasmid DNA and
digest separate portions of it with each of two different
restriction enzymes, BamHI and HpaI, and also perform a double
digest using both enzymes. You then fractionate the enzyme digests
on an agarose gel and stain the gel with ethidium bromideto
visualize the restriction fragment patterns. Your results are shown
below. Size markers (in nucleotide base pairs)...
Genetics Restriction Mapping
A 7.0kb plasmid is cut at a Pstl site and a 4.0kb piece of DNA is inserted forming a recombinant plasmid. A restriction digest is conducted and then visualized with agarose gel electrophoresis. Construct a plasmid map of the recombinant fragment using the foliowing size information (in kb about the cuts produced by the digest. Map this plasmid. The way to present your answer is very specific, see example below. 7.0 Pstl 4.0 6.0 ECORI 5.0 8.9...
can
someone explain throughly on how to find a-c??? thanks!!!
The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
You have discovered a new plasmid in a species of foul-smelling, sewer-dwelling bacteria. As a first step towards understanding the composition of this plasmid, you digest the plasmid with a series of restriction enzymes, either alone or in combination. Using agarose gel electrophoresis, you determined the size of each fragment produced in these reactions and listed the result in the table below: A. How large is the plasmid in bp? B. Using the circle (plasmid) below, draw a restriction map...
Unanswered Question 6 0/1 pts A restriction digest is performed on a plasmid. The table below shows the sizes of the resulting DNA fragments. 3 enzyme(s) present in the DNA size fragments digest resulting Pstl 10,000 base pairs (bp) Sspl 7,000 bp, 3,000 bp double digest, Estland 4,000 bp, 3,000 bp Sspl Explain the double digest results, what happened to create pieces of these sizes? Your Answer: 4/4 pts Question 7 List the 5 components needed to perform a PCR...
B4. Answer ALL parts: The diagram below shows the restriction pattern of a plasmid cut with the enzymes BamHI, EcoRI and Ndel. The digests are carried out with each enzyme alone and then with different combinations of the three enzymes. Enzyme Fragment sizes kb BamHI 1.4 10.6 EcoRI 4.5 7.5 Ndel 2.5 9.5 BamHI + EcoRI 45 3.4 1.4 BamHI + Ndel 1.4 2.5 5.9 EcoRI + Ndel 0.5 2.0 2.5 7.0 (a) Draw an agarose gel with restriction fragments...
Most questions answered within 3 hours.
-
The differences between the two sets of dependent data are -1,
2,-,2, 2. Round to the...
asked 9 minutes ago -
A χ2-curve, looking at the relationship between age and hours
spent working at an office per...
asked 41 minutes ago -
The pH of a sample of water from a river is 5.0. A
sample of effluent from...
asked 1 hour ago -
At the beginning of the period, the Fabricating Department
budgeted direct labor of $136,500 and equipment...
asked 1 hour ago -
Please answer all
____ 28. Rent control is usually
justified on the grounds that it protects...
asked 1 hour ago -
PARTS A-D HAVE BEEN ANSWERED. WAS TOLD TO REPOST. ONLY ANSWER
PARTS E and F.
A...
asked 2 hours ago -
2) You are given the task of finding a representation for a
circle in a drawing...
asked 3 hours ago -
STUDY QUESTION: Does use of diet drug fen-phen
(fenfluramine-phentermine) cause valvular heart disease?
HINT: Valvular heart...
asked 3 hours ago -
1. An object weighing 40 N rests on a surface. The coefficient
of friction is 0.35....
asked 4 hours ago -
Investor company owns 35% of investee company voting stock and
accounts for the investment under the...
asked 5 hours ago -
The number of major faults on a randomly chosen 1 km stretch of
highway has a...
asked 5 hours ago -
Consider the competitive environment of Starbuck's, Progressive
Insurance, a manufacturing firm with low turnover, or a...
asked 6 hours ago