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Draw a process of integration of an F plasmid to the E. coli genome shown below....
cells bacteria) do not contain the F plasmid. Position the In E. coli, F cells (male...
cells bacteria) do not contain the F plasmid. Position the In E. coli, F cells (male bacteria) contain the F plasmid, whereas F labels below with the correct images of conjugation in E. coli. After conjugation, both cells are F and can conjugate with F" cells. One strand of the plasmid passes through the pilus and into the female cell An F pilus produced by the male cell contacts and binds to the cell wall of a female cell. The...
The plasmid pFR55 is a useful vehicle for the cloning of any DBA sequence in E....
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
Knowing the genome size of E. coli and the fact that it can divide ca. every...
Knowing the genome size of E. coli and the fact that it can divide ca. every 20 minutes (under optimal growth conditions), calculate the rate of DNA replication in bp/sec (assume only one origin of replication and bidirectional replication). use 4.385 x 10^-9 as the weight in micrograms for the genome size
Answer both a and b. The genome of the bacteria E. coli is 5.4 X 106...
Answer both a and b. The genome of the bacteria E. coli is 5.4 X 106 nucleotides long. Replication of the DNA here is 500 nucleotides/second. Replication is bi-directional from the single origin, so each fork copies ~ half of the DNA and the two forks probably get done at about the same time. a. How long should it take (in minutes) for replication to be completed in E. coli? The haploid human genome is 3 X 109 nucleotides long,...
2. (Multiple answers) A student is doing an experiment to insert the plasmid seen below into...
2. (Multiple answers) A student is doing an experiment to insert the plasmid seen below into an E. coli bacteria. They cut their plasmid and gene of interest with the HIND III restriction enzyme to insert their gene of interest. lacz HindIII BamHI -EcoRI amp PUC19 After the experiment is complete they observe many blue colonies on their plate media that contained nutrients, X-gal and ampicillin. Please choose all that are TRUE about this experiment. ampicillin is the selective ingredient...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and – designates a deletion of that gene or site; lacO+ means all operators (O1, O2 and O3) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of lac operon in E.coli. b. Role of CAP and cAMP the activator binding site c. Role of Lac Repressor and Operator and Inducer d. Role of Looping e. Effect of mutations f. Recessive v dominant mutations g. Complementing v noncomplementing mutations. Describe the regulation of the trp operon in E. coli. Describe at least three kinds of molecular switches involved in gene regulation. What...
As a student project, you have isolated six new mutant strains of E. coli with altered...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
cells bacteria) do not contain the F plasmid. Position the In E. coli, F cells (male bacteria) contain the F plasmid, whereas F labels below with the correct images of conjugation in E. coli. After conjugation, both cells are F and can conjugate with F" cells. One strand of the plasmid passes through the pilus and into the female cell An F pilus produced by the male cell contacts and binds to the cell wall of a female cell. The...
The plasmid pFR55 is a useful vehicle for the cloning
of any DBA sequence in E. coli. A restriction map of pFR55 showing
the restriction enzyme cutting sites is shown below. The plasmid
can replicate I'm E. coli and carries the tetracycline resistance
Tc and ampicillin resistance (Ap) genes for use as selectable
markers in E. coli.
a. you wish to insert a gene into the SalI site of
pFR55. How would you select for E. Coli cells that have...
2. (Multiple answers) A student is doing an experiment to insert the plasmid seen below into an E. coli bacteria. They cut their plasmid and gene of interest with the HIND III restriction enzyme to insert their gene of interest. lacz HindIII BamHI -EcoRI amp PUC19 After the experiment is complete they observe many blue colonies on their plate media that contained nutrients, X-gal and ampicillin. Please choose all that are TRUE about this experiment. ampicillin is the selective ingredient...
Shown below are relevant genes and sites from various E. coli strains. Note: + designates the wild-type gene or site that is fully functional, and - designates a deletion of that gene or site; lacot means all operators (01, O2 and 03) are functional; assume all other genes and sites not listed are wild-type and functional (including lacA). In some cases, a plasmid containing a wild-type functional gene or site was transformed into the E. coli strain. The strains were...
Chromosome Figure 6 Fiqure 7 R plasmid F plasmid integrated into host chromosome Sex pilus chromosome of recipient Figure 8 Double- Cel stranded Cytoplasmic ll ONA Integrated DNA Treat with electric current Recipient cell Fragments of donor DNA Transformed cell Phage Figure 9 Figure 10 Phage DNA enters the cell Bacterial host #1 앗 O E. coli F plasmid Gov Integration of F plasmid Bacterial DNA -Bacterial host #2 85 Replaced host DNA Bacterial DNA I Which diagram(s) depict conjugation?...
As a student project, you have isolated six new mutant strains
of E. coli with altered behavior of the lactose operon. The strains
are listed in the table below, together with their phenotypes (with
regard to significant ?-galactosidase synthesis) in three specific
situations.
Columns 1 and 2 present the phenotypes of each mutant haploid
strain. In column 1, the mutant is in an otherwise wild-type
genome. In column 2, the genome also carries a nonsense-suppressor
mutation (that is not present...
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