3. Why would it be a good idea to remove rRNA from your total RNA sample...
3. Why would it be a good idea to remove rRNA from your total RNA sample when evaluating the transcriptome using bioinformatics (gene sequencing)?
Homework Answers
Next Generation Sequencing technologies have revolutionized gene expression studies and functional geneomics analysis.However further improvment of RNA sequencing protocols is till desirable inorder to reduce cost and increse its accuracy. In bacteria a major problem in RNA sequencing is the abundance of ribosomal RNA (rRNA) which accouts to 95-98% of total RNA and therefore hinder sufficent covarage of mRNA , the main focus of transcriptome studies.Thus efficient removel of rRNA is necessary to achive optimal covarage good detection sensitivity and reliable results.
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3. Why would it be a good idea to remove rRNA from your total
RNA sample...
To find RPKM in RNA seq you first add up your total reads in a gene...
To find RPKM in RNA seq you first add up your total reads in a gene and divide by your scaling factor, a number decided by the scientist based on intended sequencing depth. The next step is to divide then divide by the length of the gene in kilobases. If the scaling factor was 1,000,000 this gives you RPKM or reads per kilobase million. Is this correct? What does RPKM represent/ what kind of practical information does it give us?...
Why is it a good idea to extend your bare hand forward when you are getting...
Why is it a good idea to extend your bare hand forward when you are getting ready to catch a fast-moving baseball? Answer:So you can pull your hand back, increasing the time to slow the ball thus deceasing the force.
PART 3: Designing a Guide RNA You will now design your own guide RNA to inactivate...
PART 3: Designing a Guide RNA You will now design your own guide RNA to inactivate a butterfly gene like Robert Reed did. Your goal is to knock out the optix gene in a species of butterfly called the painted lady (Vanessa cardu). Activity Stander Biointeractive Using CRISP other Your guide RNA must match a target DNA sequence in the gene that you want to knock out. A partial sequence of an exon from the butterfly's aptix gene is shown...
5.) Why would the mitochondrion go to all of the trouble of hanging on to genes...
5.) Why would the mitochondrion go to all of the trouble of hanging on to genes that code for tRNA when it would easier to import the tRNA molecules from its host cell? Like mad stupid or what? 6.) In Introductory Biology (and Chemistry), you are typically taught 'RNA language', since there is no thymine, A pairs with U and C pairs with G. Name one area of RNA that contains thymine. 7.) What is the function of wobble? EC!...
Once a bacterial 16s rRNA gene has been amplified, it is possible to have the nucleotide...
Once a bacterial 16s rRNA gene has been amplified, it is possible to have the nucleotide sequence of the DNA determined. There are many different techniques for this. In lab, you isolated and amplified DNA from a known organism (Escherichia coli), but what if you had to identify an unknown organism? You would send the sample away for sequencing and then once you got the results you would “BLAST” the sequence. Using the Standard Nucleotide BLAST on NCBI and the...
1. Why is it a good idea to scale your position, and have larger positions when...
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3. Which of the following is not a good example of a housekeeping protein in eukaryotic...
3. Which of the following is not a good example of a housekeeping protein in eukaryotic cells? a)DNA polymerase b)histones c)RNA polymerase 4)hemoglobin Explanation: 4. Which of the following statements about histones is true? a. Histones activate transcription when bound to the promoter, b. Although RNA polymerase can access DNA packed around histones, the general transcription factors and transcriptional regulators cannot. c. Histone acetyltransferases affect transcription by adding acetyl groups to histones and altering chromatin structure to allow accessibility to...
Question 2a If the DNA template 5′- ATGGATGC -3′ is transcribed to RNA, the RNA would...
Question 2a If the DNA template 5′- ATGGATGC -3′ is transcribed to RNA, the RNA would be best described as... a. 3′- TACCTACG -5′. b. 5′- ATGGATGC -3′. c. 5′- AUGGAUGC -3′. d. 5′- UACCUACG -5′. e. 3′- UACCUACG -5′. Question 2b Which answer best summarizes how eukaryotic and bacterial RNA polymerases are different? a. Eukaryotes have several types of multimeric RNA polymerases, whereas bacteria only have one monomeric RNA polymerase. b. Eukaryotes have several types of RNA polymerases, one...
Why would there be duplicate invoice numbers? CaseWare IDEA Duplicate2 Sample-Detailed Sales Duplicate x INV NO...
Why
would there be duplicate invoice numbers?
CaseWare IDEA Duplicate2 Sample-Detailed Sales Duplicate x INV NO INV DATE SALESREP NO CUSTNO PROD CODE UNIT PRICE QTY SALES BEF TAX SALES TAX SALES 1 1000097 3/6/2015 3 1000109 3/27/2015 7 1000140 2/7/2015 si 30228 05 12 1000350 7/6/2015
molecular biology Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory r...
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
PART 3: Designing a Guide RNA You will now design your own guide RNA to inactivate a butterfly gene like Robert Reed did. Your goal is to knock out the optix gene in a species of butterfly called the painted lady (Vanessa cardu). Activity Stander Biointeractive Using CRISP other Your guide RNA must match a target DNA sequence in the gene that you want to knock out. A partial sequence of an exon from the butterfly's aptix gene is shown...
5.) Why would the mitochondrion go to all of the trouble of hanging on to genes that code for tRNA when it would easier to import the tRNA molecules from its host cell? Like mad stupid or what? 6.) In Introductory Biology (and Chemistry), you are typically taught 'RNA language', since there is no thymine, A pairs with U and C pairs with G. Name one area of RNA that contains thymine. 7.) What is the function of wobble? EC!...
3. Which of the following is not a good example of a housekeeping protein in eukaryotic cells? a)DNA polymerase b)histones c)RNA polymerase 4)hemoglobin Explanation: 4. Which of the following statements about histones is true? a. Histones activate transcription when bound to the promoter, b. Although RNA polymerase can access DNA packed around histones, the general transcription factors and transcriptional regulators cannot. c. Histone acetyltransferases affect transcription by adding acetyl groups to histones and altering chromatin structure to allow accessibility to...
Why
would there be duplicate invoice numbers?
CaseWare IDEA Duplicate2 Sample-Detailed Sales Duplicate x INV NO INV DATE SALESREP NO CUSTNO PROD CODE UNIT PRICE QTY SALES BEF TAX SALES TAX SALES 1 1000097 3/6/2015 3 1000109 3/27/2015 7 1000140 2/7/2015 si 30228 05 12 1000350 7/6/2015
molecular biology
Section C (40 marks) Answer ALL questions from this Section 5. You have isolated total RNA from muscle cells and constructeda muscle cDNA library. You wish to study the regulatory region of a muscle-specific cDNA gene (gene M) that you have previously identified. 6 (a) For your study, you need to isolate a genomic clone of gene M. Why isa cDNA clone of gene M not appropriate for your study? (2 marks) (b) Outline the steps you would...
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