In sequencing, it is key that all primer molecules hybridize to the exact same 3’ base...
Homework Answers
The primer molecules are the short oligonucleotides RNA sequences that provides an existing nucleotide sequence for the DNA polymerase to initiate polymerization. The replication always occur in the direction of 5'-3' direction. So, the primer molecule binds to the 5'-end of the template, with the complimentary 3'-end of the primer.
Therefore, the direction of the template is 5'-3' and the direction of primer is 3'-5'.
When the primer molecules hybridize to the template, the enzyme DNA polymerase begins the polymerization.
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In
sequencing, it is key that all primer molecules hybridize to the
exact same 3’ base...
The following primer sequence was used in a Sanger sequencing reaction along with the following template,...
The following primer sequence was used in a Sanger sequencing reaction along with the following template, how many different products would be expected if the only dideoxynucleotide being used was ddTTP? Primer: 5’ TGGCGCGC 3’ Template: 5’ TGGCGCGCATGGTTTAGCGCGCCA 3’ a. 6 b. 5 c. 4 d. 3 e. 2
THANK YOU!! Target Region Heated to 94°C DNA is denatured GGGCAACGTG CATCACTTTG Primers hybridize with template...
THANK YOU!!
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The following strand of DNA is to be used for sequencing: 3'CGACTGACTCGACGGCCTAGCTATAG. The primer is CTGACTG....
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Sanger sequencing allows the identification of the bases and their order in a target piece of...
Sanger sequencing allows the identification of the bases and their order in a target piece of DNA. This technique exploits the way DNA is transcribed by using a primer and allowing new DNA to be created from its 3' end. In this technique, DNA is manufactured in vitro, which means that enzymes and molecules are combined in a test tube to allow the reactions to occur. In the original technique, four tubes were prepared, each containing the same reactants, but...
Using the key provided (in the first photo on the right hand side) interpret the nucleotide...
Using the key provided (in the first photo on the right hand
side) interpret the nucleotide base sequences in the gour
electropherograms below. Write each base below its corresponding
peak.
Also, are the sequences above idential to the DNA template? Why or
why not?
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its corresponding peak. - 2pts KEY...
Short and clear answers please! Thank you Question 2: Advances in DNA sequencing, 5 points A....
Short and clear answers please! Thank you
Question 2: Advances in DNA sequencing, 5 points A. 3 points. Imagine you are explaining the revolution in DNA sequencing to a relative. You're trying to explain to them why the price dropped one-million fold. The major key advance that lead to this drop in price is: B. 1 point. In Next Generation Sequencing, dsDNA linkers are added to each of the fragmented pieces of DNA. This linker enables the fragments to stick...
THANK YOU!!
Target Region Heated to 94°C DNA is denatured GGGCAACGTG CATCACTTTG Primers hybridize with template Temperature is lowered After one round of PCR the number of double-stranded DNA molecules is doubled. TGG TCACTTTGGCAAIAGAATT Heat-stable DNA polymerase adds nucleotides to the 3' end of the primer Primer 1 Primer 2 Heated to 72°C 5 TGCCTGGCCCCATCACTTTGGCAA AGAATTC 5
The following strand of DNA is to be used for sequencing: 3'CGACTGACTCGACGGCCTAGCTATAG. The primer is CTGACTG. The number of DNA fragments resulting from a tube containing all the normal deoxyribonucleotides along with dideoxyCTP added is expected to be: 06 05 08
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its KEY corresponding peak. - 2pts And memes 1. Wallet 2 banan sam hesalesanan Amalan 3. 4. 12 V Α' Α' Aav po e te AL А. ab x *² ADA Style 5. Are the sequences above identical to the DNA Template? Why or why not? -1pt
3. (2 pts.) You are sequencing the end of a genomic DNA fragment that was cut with the restriction enzyme BamH1 and ligated into a plasmid that was also cut with BamH1. You have denatured the DNA and annealed a labeled primer to plasmid sequences adjacent t<o the insertion site as shown below: 3' BamHi 5' GATCTAGCTAGCTAGCTAGCTAGCTAGCTAGCCATCGATGCTAGGAATCTTTGCTGATGCTAGTCGATGCCGTAGC ACTACGATCAGCTACGGC 3' 18 base primer 5' Next you add DNA polymerase, buffer, an excess of all four dNTPs, and a small amount of...
Sanger sequencing allows the identification of the bases and their order in a target piece of DNA. This technique exploits the way DNA is transcribed by using a primer and allowing new DNA to be created from its 3' end. In this technique, DNA is manufactured in vitro, which means that enzymes and molecules are combined in a test tube to allow the reactions to occur. In the original technique, four tubes were prepared, each containing the same reactants, but...
Using the key provided (in the first photo on the right hand
side) interpret the nucleotide base sequences in the gour
electropherograms below. Write each base below its corresponding
peak.
Also, are the sequences above idential to the DNA template? Why or
why not?
POST-LAB #9 - DNA Barcoding: Animal Phylogeny Building by Sequencing (20pts) Directions: Using the key provided, interpret the nucleotide base sequences in the four electropherograms below. Write each base below its corresponding peak. - 2pts KEY...
Short and clear answers please! Thank you
Question 2: Advances in DNA sequencing, 5 points A. 3 points. Imagine you are explaining the revolution in DNA sequencing to a relative. You're trying to explain to them why the price dropped one-million fold. The major key advance that lead to this drop in price is: B. 1 point. In Next Generation Sequencing, dsDNA linkers are added to each of the fragmented pieces of DNA. This linker enables the fragments to stick...
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