Recognition - MutS
Excision - MutH
DNA synthesis -DNA poly III
Ligation - DNA ligase
Note- the MutS is use to detect or recognize the mismatch bases on daughter strand And bind the mutated DNA. MutH bind to the hemimethylated site on daughter DNA , the mutS DNA complex act as mediator between MUt H and mutS2 thus the whole mutSHL complex then slide over the DNA in direction of mismatch , liberating the strand, excised as it goes.
DNA poly IIIIs used in synthesis of new strand of DNA along with ATP and Mg+, this enzyme help to add nucletide in 3' end of chain so that DNA strand formation occur in 5' to 3' direction.
The okazaki fragment are joined together by DNA ligase the strand of these fragment is lagging strand.
Sort the protein(s) that complete each of the steps in E, coli foe the mismatch repair...
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10 per base pair per round of replication. Based on this, how many mutations per base pair would you expect to see in a population of 1010 E. coli cells (e.g. of a TSB culture of E. coli)
describe how the Methyl-directed mismatch repair system in E. coli which DNA strand is the correct strand and determines which DNA strand has the mutation. describe how DNA methylation is heritable during replication how epigenetic modifications are involved in genomic imprinting, X-inactivation, and regulation of tissue/cell specific gene expression (including the general roles of TrxG and PcG group proteins). ***Are these heritable during mitosis or meiosis? Are these reversible? Can you support your answer?
A new strand of E. coli has an overactive DNA-methylase enzyme. what repair pathway will be impacted by this overexpression of DNA demethylase and how is it impacted
The E. coli Fur protein is - a constitutive protein. - a regulatory protein. - an allosteric protein. - a repressor protein. A. One of the above. B. Two of the above. C. Three of the above. D. All of the above.
Methionine is an amino acid made by E coli. The met (methionine) operon in E. coli responds to a repressor protein which itself is: a. encoded by the operon b. turned off when bound to methionine c. turned on when bound to methionine d. unresponsive to any product of the methionine biosynthetic pathway e. turned on when bound to tryptophan
Expressing eukaryotic genes in E. coli: Describe important steps (include various enzymes and their functions) needed to clone and express proinsulin in E. coli (exclude purification of the protein)
Order steps 1-7, first step (1) and last step (7) Order the events in the repair of a GT base pair in E. coli. • The three-component complex (MutSLH) identifies the old strand by locating a methylation mark. The endonuclease then cleaves the unmethylated (new) strand. • The mismatch-binding protein complex (MutSL) scans the genome after DNA replication to find incorrect base pairs. • DNA polymerase extends the 3'OH of the unmethylated, new strand to synthesize new DNA. • Once...
In nucleotide excision repair in E. coli, what is the function of the UvrA/UvrB complex?
In a lacI- strain of E. coli (the repressor protein is not expressed in this mutant) the lac operon will be expressed...
Expression of a heterologous protein in CHO cells and in an E. coli system produced proteins with the same molecular weight on SDS-PAGE. Subsequent in vitro bioactivity studies demonstrated high-level activity with the CHO cell-derived recombinant protein, but no bioactivity with the E. coli derived product. Explain this discrepancy, and propose an experiment to test your hypothesis