Question

Sort the protein(s) that complete each of the steps in E, coli foe the mismatch repair pathway.

Answer 1

Recognition - MutS

Excision - MutH

DNA synthesis -DNA poly III

Ligation - DNA ligase

Note- the MutS is use to detect or recognize the mismatch bases on daughter strand And bind the mutated DNA. MutH bind to the hemimethylated site on daughter DNA , the mutS DNA complex act as mediator between MUt H and mutS2 thus the whole mutSHL complex then slide over the DNA in direction of mismatch , liberating the strand, excised as it goes.

DNA poly IIIIs used in synthesis of new strand of DNA along with ATP and Mg+, this enzyme help to add nucletide in 3' end of chain so that DNA strand formation occur in 5' to 3' direction.

The okazaki fragment are joined together by DNA ligase the strand of these fragment is lagging strand.