A new strand of E. coli has an overactive DNA-methylase enzyme. what repair pathway will be...
A new strand of E. coli has an overactive DNA-methylase enzyme. what repair pathway will be impacted by this overexpression of DNA demethylase and how is it impacted
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Answer :)
DNA methylation is essential for gene regulation and cell differentiation but in a regulatory method. DNA methylation protects DNA to bind with replication or transcription proteins but demethylation is also important to cause differentiation of cells. Methylation and demethylation both are important for the survival of cells.
DNA methylation is important in mismatch repair of the new unmethylated strand. However, the overexpression of DNA methylase causes premature methylation. This situation brings mutations in newly synthesized DNA because the premature methylated strand cannot be repaired.
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A new strand of E. coli has an overactive DNA-methylase enzyme.
what repair pathway will be...
describe how the Methyl-directed mismatch repair system in E. coli which DNA strand is the correct...
describe how the Methyl-directed mismatch repair system in E. coli which DNA strand is the correct strand and determines which DNA strand has the mutation. describe how DNA methylation is heritable during replication how epigenetic modifications are involved in genomic imprinting, X-inactivation, and regulation of tissue/cell specific gene expression (including the general roles of TrxG and PcG group proteins). ***Are these heritable during mitosis or meiosis? Are these reversible? Can you support your answer?
What are two major factors that contribute to DNA double-strand break repair pathway choice(HDR, NHEJ, or...
What are two major factors that contribute to DNA double-strand break repair pathway choice(HDR, NHEJ, or Alt.NHEJ)? Please explain
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’
One strand of a section of DNA isolated from E. coli reads: 5’GTAGCCTACCCATAGG-3’ (a) Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? (b) What is the amino acid sequence of the proteins. (c) Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not. (d) If the T underlined in the above sequence was to...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? What is the amino acid sequence of the proteins? Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not? If the T underlined in the above sequence was to go...
10. During repairs the mismatch repair machinery has to distinguish the old DNA strand from the...
10. During repairs the mismatch repair machinery has to distinguish the old DNA strand from the new strand, how does it do this?
To place the E. coli replication system on the human scale, imagine that the DNA strand...
To place the E. coli replication system on the human scale, imagine that the DNA strand type B has a diameter of 20 angstroms and that it expands to 1m in diameter. If this expansion happened for the holoenzyme DNA polymerase III, it would be about the size of almost half a truck. In this expanded system. (a) How fast each replisome would move. (b) How far would each replisome travel? (c) What size would an Okasaki fragment be? (d)...
Identify the various types of DNA repair mechanisms known to counteract the effects of UV radiation....
Identify the various types of DNA repair mechanisms known to counteract the effects of UV radiation. Recombinational repair Excision repair Photoactivation repair SOS repair 1. is dependent on a photon-activated enzyme that cleaves thymine dimers. 2. is the process by which an endonuclease clips out UV- induced dimers, DNA polymerase III fills in the gap, and DNA ligase rejoins the phosphodiester backbone. 3. uses the corresponding region on the undamaged parental strand of the same polarity. 4. is a process...
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10...
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10 per base pair per round of replication. Based on this, how many mutations per base pair would you expect to see in a population of 1010 E. coli cells (e.g. of a TSB culture of E. coli)
1) When DNA is damaged, a cell has a variety of repair pathways available to correct...
1) When DNA is damaged, a cell has a variety of repair pathways available to correct the problem. Discuss their timing, anything they are good at detecting, and explain their specific mechanisms. A) 3pts - Mismatch repair: B) 3pts - Base excision [BER] repair: C) 3pts - Strand breakage repair – compare HRR [homologous recombination repair] and NREJ [non-recombination end joining] 2) 5pts - Discuss how an E. coli origin of replication is recognized [sequences?], opened [by dnaA], and...
4. Given the following information: One strand of a section of DNA isolated from E. coli...
4. Given the following information: One strand of a section of DNA isolated from E. coli reads 5’-GTAGCCTACCCATAGG-3’ 3’ CATCGGATGGGTACC’5 Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be (please include orientation)? How many different polypeptides chains could potentially be made from this sequence of RNA? Would the same polypeptide chains be made if the other strand of the DNA...
One strand of a section of DNA isolated from E. coli reads: Suppose that an mRNA is transcribed from this DNA using the complementary strand as a template. What will the sequence of the mRNA in this region be? What is the amino acid sequence of the proteins? Would the same proteins be made if the other strand of DNA served as template for transcription? Why or why not? If the T underlined in the above sequence was to go...
Identify the various types of DNA repair mechanisms known to counteract the effects of UV radiation. Recombinational repair Excision repair Photoactivation repair SOS repair 1. is dependent on a photon-activated enzyme that cleaves thymine dimers. 2. is the process by which an endonuclease clips out UV- induced dimers, DNA polymerase III fills in the gap, and DNA ligase rejoins the phosphodiester backbone. 3. uses the corresponding region on the undamaged parental strand of the same polarity. 4. is a process...
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