10. During repairs the mismatch repair machinery has to distinguish the old DNA strand from the new strand, how does it do this?
DNA is methylated, before DNA replication both strands of DNA are methylated but after replication, the newly formed strand is not methylated so DNA is hemimethylated so mismatch repair machinery distinguishes the new strand from old strand based on the methylation pattern, the strand which is not methylated is the new strand.
10. During repairs the mismatch repair machinery has to distinguish the old DNA strand from the...
DNA mismatch repair enzymes preferentially repair bases on the newly synthesized DNA strand, using the old DNA strand as the template. If mismatches were simply repaired without regard for which strand served as template, would this reduce replication errors? Explain your answer (5 pts) Describe DNA replication (remember to mention all the enzymes involved) (10 pts)
describe how the Methyl-directed mismatch repair system in E. coli which DNA strand is the correct strand and determines which DNA strand has the mutation. describe how DNA methylation is heritable during replication how epigenetic modifications are involved in genomic imprinting, X-inactivation, and regulation of tissue/cell specific gene expression (including the general roles of TrxG and PcG group proteins). ***Are these heritable during mitosis or meiosis? Are these reversible? Can you support your answer?
10. (1 pr all or none) Concering methyl-directed mismatch repair circle all FALSE statements: a. In methyl-directed mismatch repair the strand that is repaired is the methylated strand. b. DNA Poll is required to fill in the repair gap. c. The Muth protein is directly responsible for recognizing mismatched base pairs.
Mismatch repair requires all of the following EXCEPT: Requires information from a nearby homologous chromosome Uses information on the parental strand to identify the error. Is tightly associated with new replicated DNA Makes use of ligase. Requires the use of a repair DNA polymerase. Question 12 Which of the following is NOT necessary for homologous recombination to occur? O nucleases 5' DNA strand overhangs 3' DNA strand overhangs ligase a long stretch of sequence similarity Which of the following apply...
Question 11 1 pts Mismatch repair requires all of the following EXCEPT: Requires the use of a repair DNA polymerase. O is tightly associated with new replicated DNA O Requires information from a nearby homologous chromosome O Makes use of ligase. Uses information on the parental strand to identify the error.
A new strand of E. coli has an overactive DNA-methylase enzyme. what repair pathway will be impacted by this overexpression of DNA demethylase and how is it impacted
1. If a template strand reads: 3’-TTG CAA TGC AAC-5’ what will the new strand read? 2. How are new nucleotide monomers attached to the growing strand? What is the reaction that takes place? How is dATP different from ATP? 3. Why does the lagging strand exist? 4. In the lagging strand, what is the enzyme that replaces the RNA primer with DNA? What enzyme then connects the two fragments together? 5. The replication machinery is very accurate but every...
5. About double strand DNA repair, it is correct to say that choose the most appropriate answer): (a) It requires one intact strand as a template for error correction. (b) Mismatches in the DNA are usually corrected via double strand DNA repair mechanisms. (c) Homologous recombination usually results in DNA repair with no loss of nucleotide at repair site. (d) Non-homologous end-joining usually results in DNA repair with no loss of nucleotide at repair site. 6. A eukaryote gene has two introns and three exons....
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10 per base pair per round of replication. Based on this, how many mutations per base pair would you expect to see in a population of 1010 E. coli cells (e.g. of a TSB culture of E. coli)
z Instructions Question 1 (Q039) In addition to the repair of DNA double-strand breaks, homologous recombination is a mechanism fe generating genetic diversity by swapping segments of parental chromosomes. During which process does swapping occur? DNA replication ODNA repair O meiosis transposition No new data to save. Last checked at 8:05am