You have a highly concentrated stock solution and to make the working solution you must dilute the stock by a total 1: 10,000. To make this dilution you need to make step dilutions. What two dilutions can you make?
Steps:
1. Initially, combine 1 unit volume stock solution with 99 unit vol. diluting agent i.e. 1:100 dilution.
2. In the next step, combine 1 unit volume of 1:100 dilution with 99 unit volume diluting agent i.e 1:100*100= 1: 10,000
Thus converting the stock in 1:1000 dilution working stock in two steps.
You have a highly concentrated stock solution and to make the working solution you must dilute...
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