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QUESTION 5 Arrange the following objective lenses in the increasing order of their magnification power. High...
4. What is the total magnification you achieve using the following lenses? Total magnification Lens 10x...
4. What is the total magnification you achieve using the following lenses? Total magnification Lens 10x ocular lens 4X scanning objective lens 10X low-power objective lens 40X high-power objective lens 100X oil-immersion objective lens
2. How is tutai -aagnification calculated? What is the total magnification of the low-power objective? 3....
2. How is tutai -aagnification calculated? What is the total magnification of the low-power objective? 3. Define working distance 4. What happens to working distance as total magnification increases? Activity 3: Determining Field Diameter and Estimating the Size of Objects 1. Perform the following conversions. Am c.350 pm Based on the calculations that you made for your microscope, what is the field diameter for the a. scanning objective? b. low-power objective? c. high-power objective? d. oil-immersion objective? 2. um 3....
Lab Manual Total magnification is calculated by multiplying the magnification of the ocular lens (eyepiece) by...
Lab Manual Total magnification is calculated by multiplying the magnification of the ocular lens (eyepiece) by the magnification of the objective lens. The magnitication of a lens is imprinted on the lens casing. Total Magnification Observation: Total Magnification Calculate total magnification figures for your microscope, and record your findings in Table 2.3. Total Magnification Table 2.3 Total Magnification Objective Lens Ocular Lens Objective Scanning power (if present) Low power High power Oil immersion (if presents A microscope's field of view...
Biology ercise 1: Using a Light Microscope: 1. Fill out this table to calculate total magnification...
Biology
ercise 1: Using a Light Microscope: 1. Fill out this table to calculate total magnification using different objectives: Total Magnification Name of Objective Scanning Low Power High Power Oil Immersion Objective Magnification 4x 10x 40x 100x 1. Use these data to answer the questions: Solution B Absorbance Value 0.235 0.749 0.182 0.541 a. Which solution has the highest concentration? b. Which solution has the lowest concentration?
3. In a bright-field compound microscope, starting from the illuminator, follow the pathway of light: a....
3. In a bright-field compound microscope, starting from the illuminator, follow the pathway of light: a. The first lens that provides magnification of the image is the c lor lens. b. The lens that further enlarges the image ten more times is the low power nel lens. 4. Open the iris diaphragm all the way while using the low power objective. What happens to the field of view and the quality of the image? Now close the iris diaphragm all...
C. Answer the following questions as they relate to the microscope. What is the difference between...
C. Answer the following questions as they relate to the microscope. What is the difference between magnification and resolution? Why are both necessary for microscopy? What is the function of the oil used with the oil-immersion lens? What is the effect of omitting it? Why is it imperative to promptly remove any oil that has accidentally spilled on the other non-immersion lenses? D. Examination of Living Bacteria From your observation of the hay infusion, draw and describe what you have...
EXERCISE 1: USE AND CARE OF THE MICROSCOPE 13 0 Move the high-dry lens out of...
EXERCISE 1: USE AND CARE OF THE MICROSCOPE 13 0 Move the high-dry lens out of position, and place a drop of immersion oil on the area of the slide you are observing. Carefully click the oil immersion lens into position. It should now be immersed in the oil (FIGURE 1.5). Careful use of the fine-adjustment knob should bring the object into focus. Note the shape and size of the cells. Did the color of the cells change with the...
Lab activities and worksheets Read through this section before you get to lab so you are...
Lab activities and worksheets Read through this section before you get to lab so you are aware of what you will be doing for this lab. Microscope labeling It is important to know the structure of something in order to know how it works and how to use it. Use the following list of parts of a compound microscope to label the diagram. Be sure to understand the functions of each part. Ocular: this is the lens at the top...
QUESTION 3 Which of the following light microscopes require staining to view details of a fixed...
QUESTION 3 Which of the following light microscopes require staining to view details of a fixed specimen on a light background? Inverted Microscope All of these Phase Contrast Microscope Confocal Microscope Darkfield Microscope Brightfield Microscope QUESTION 4 Which of the following would be the proper use of a microscope? Select all that apply. Move the microscope by carrying it by the arm and the base. After use turn off the light and unplug the microscope After using the oil immersion...
1. Name at least 3 ways you can control the amount of light that reaches the...
1. Name at least 3 ways you can control the amount of light that reaches the ocular lens. 2. Define resolution. What is the limit of resolution for the light microscope? 3. What effect does the wavelength of light used have on resolution? 4. If you view a bacterial smear under the oil immersion lens, but forget to add oil, will the image still be just about as clear as if you had added the oil. 5. Define parfocal. 6....
4. What is the total magnification you achieve using the following lenses? Total magnification Lens 10x ocular lens 4X scanning objective lens 10X low-power objective lens 40X high-power objective lens 100X oil-immersion objective lens
2. How is tutai -aagnification calculated? What is the total magnification of the low-power objective? 3. Define working distance 4. What happens to working distance as total magnification increases? Activity 3: Determining Field Diameter and Estimating the Size of Objects 1. Perform the following conversions. Am c.350 pm Based on the calculations that you made for your microscope, what is the field diameter for the a. scanning objective? b. low-power objective? c. high-power objective? d. oil-immersion objective? 2. um 3....
Lab Manual Total magnification is calculated by multiplying the magnification of the ocular lens (eyepiece) by the magnification of the objective lens. The magnitication of a lens is imprinted on the lens casing. Total Magnification Observation: Total Magnification Calculate total magnification figures for your microscope, and record your findings in Table 2.3. Total Magnification Table 2.3 Total Magnification Objective Lens Ocular Lens Objective Scanning power (if present) Low power High power Oil immersion (if presents A microscope's field of view...
Biology
ercise 1: Using a Light Microscope: 1. Fill out this table to calculate total magnification using different objectives: Total Magnification Name of Objective Scanning Low Power High Power Oil Immersion Objective Magnification 4x 10x 40x 100x 1. Use these data to answer the questions: Solution B Absorbance Value 0.235 0.749 0.182 0.541 a. Which solution has the highest concentration? b. Which solution has the lowest concentration?
3. In a bright-field compound microscope, starting from the illuminator, follow the pathway of light: a. The first lens that provides magnification of the image is the c lor lens. b. The lens that further enlarges the image ten more times is the low power nel lens. 4. Open the iris diaphragm all the way while using the low power objective. What happens to the field of view and the quality of the image? Now close the iris diaphragm all...
C. Answer the following questions as they relate to the microscope. What is the difference between magnification and resolution? Why are both necessary for microscopy? What is the function of the oil used with the oil-immersion lens? What is the effect of omitting it? Why is it imperative to promptly remove any oil that has accidentally spilled on the other non-immersion lenses? D. Examination of Living Bacteria From your observation of the hay infusion, draw and describe what you have...
EXERCISE 1: USE AND CARE OF THE MICROSCOPE 13 0 Move the high-dry lens out of position, and place a drop of immersion oil on the area of the slide you are observing. Carefully click the oil immersion lens into position. It should now be immersed in the oil (FIGURE 1.5). Careful use of the fine-adjustment knob should bring the object into focus. Note the shape and size of the cells. Did the color of the cells change with the...
Lab activities and worksheets Read through this section before you get to lab so you are aware of what you will be doing for this lab. Microscope labeling It is important to know the structure of something in order to know how it works and how to use it. Use the following list of parts of a compound microscope to label the diagram. Be sure to understand the functions of each part. Ocular: this is the lens at the top...
QUESTION 3 Which of the following light microscopes require staining to view details of a fixed specimen on a light background? Inverted Microscope All of these Phase Contrast Microscope Confocal Microscope Darkfield Microscope Brightfield Microscope QUESTION 4 Which of the following would be the proper use of a microscope? Select all that apply. Move the microscope by carrying it by the arm and the base. After use turn off the light and unplug the microscope After using the oil immersion...
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