The polypeptide chain of the chaperone protein GroEL from Escherichia colihas a molecular mass of 57200...
The polypeptide chain of the chaperone protein GroEL from Escherichia colihas a molecular mass of 57200 Da and does not contain tryptophan or cystines. The A280of a 1 mg/mL solution of the purified protein in a 1 cm pathlength cuvette is 0.160. calculate the number of Tyr in the polypeptide chain.
Homework Answers
Protein Concentration in mg/ml = A280*Dilution Factor*molecular weight/Extinction coefficient
1= 0.160*57200/Extinction coefficient
Extinction coefficient= 9152
for one residue of tyrosine the Extinction coefficient is 1280 so if the value is 9152 so the number of residue will be
= 9152/1280= 7.15 residues
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